Intracellular temperature measurements with fluorescent polymeric thermometers

Uchiyama, Seiichi; Gota, Chie; Tsuji, Toshikazu; Inada, Noriko

doi:10.1039/c7cc06203f

Cited by 112 publications

(91 citation statements)

References 165 publications

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“…Whereas body's entropy measures the amount of its atomic disorder, temperature measures the intensity of submicroscopic random movements of the body's constituents. The precise and accurate measurement of the temperature is vital across a broad spectrum of areas, such as automotive, aerospace and defense, metrology, climate, marine research, biomedicine and nanomedicine, electronics, heating and cooling devices, production plants, and food storage . Presently, temperature sensors account for ≈80% of the worldwide sensor market that is expected to reach to $6.86 billion by 2023, according to the Grand View Research, Inc .…”

Section: Introductionmentioning

confidence: 99%

Lanthanide‐Based Thermometers: At the Cutting‐Edge of Luminescence Thermometry

Brites

Balabhadra

Carlos

2018

Advanced Optical Materials

687

642

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Present technological demands in disparate areas, such as microfluidics and nanofluidics, microelectronics and nanoelectronics, photonics and biomedicine, among others, have reached to a development such that conventional contact thermal probes are not accomplished anymore to perform accurate measurements with submicrometric spatial resolution. The development of novel noncontact thermal probes is, then, mandatory, contributing to an expansionary epoch of luminescence thermometry. Luminescence thermometry based on trivalent lanthanide ions has become very popular since 2010 due to the unique versatility, stability, and narrow emission band profiles of the ions that cover the entire electromagnetic spectrum with relatively high emission quantum yields. Here, a perspective overview on the field is given from the beginnings in the 1950s until the most recent cutting‐edge examples. The current movement toward usage of the technique as a new tool for thermal imaging, early tumor detection, and as a tool for unveiling the properties of the thermometers themselves or of their local neighborhoods is also summarized.

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Section: Introductionmentioning

confidence: 99%

Lanthanide‐Based Thermometers: At the Cutting‐Edge of Luminescence Thermometry

Brites

Balabhadra

Carlos

2018

Advanced Optical Materials

687

642

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“…[4][5][6][7] Fluorescence imaging is a powerful method of intracellular thermometry owing to its high spatiotemporal resolution, and various types of luminescent nanothermometers have recently been developed for this purpose. [8][9][10][11] Examples include green fluorescence protein, [12] small organic molecules, [13] quantum dots, [14] polymers [15,16] and polymer dots [17] and lanthanidedoped nanoparticles. [18][19][20] Moreover, intracellular temperature mapping has revealed the existence of spatial variations in temperature within single cells.…”

Section: Introductionmentioning

confidence: 99%

Thermal Properties of Lipid Bilayers Determined Using Upconversion Nanothermometry

Bastos

Brites

Rojas‐Gutierrez

et al. 2019

Adv Funct Materials

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Luminescent nanomaterials have shown promise for thermal sensing in bio-applications, yet little is known of the role of organic coatings such as supported lipid bilayers on the thermal conductivity between the nanomaterial and its environment. Additionally, since the supported lipid bilayer mimics the cell membrane, its thermal properties are fundamentally important to understand the spatial variations of temperature and heat transfer across membranes. Herein we describe a new approach that enables direct measurement of these thermal properties using a LiYF4:Er 3+ /Yb 3+ upconverting nanoparticle encapsulated within a conformal supported lipid bilayer and dispersed in water as a temperature probe yielding the temperature gradient across the bilayer. The thermal conductivity of lipid bilayer was measured as function of the temperature, being 0.20±0.02 W·m -1 ·K -1 at 300 K. For the uncapped nanoparticles dispersed in water, the temperature dependence of the thermal conductivity was also measured in the 300-314 K range as [0.63-0.69]±0.11 W·m -1 ·K -1 . Using a lumped elements model, we calculate the directional heat transfer at each of the system interfaces, namely nanoparticle-bilayer and bilayer-nanofluid, opening a new avenue to understand the membrane biophysical properties as well as the thermal properties of organic and polymer coatings.

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“…These reports have prompted active discussions about the origin and the biological significance of the heterogeneity. However, the results have also sparked hot debates about the validity of the temperature measurements [19][20][21][22][23][24] . The issue is that the temperature rise calculated by theoretical models describing physics of cellular thermogenesis and intracellular space is several orders of magnitude smaller than the experimental values reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

In situ measurement of intracellular thermal conductivity using heater-thermometer hybrid diamond nanosensor

Sotoma

Zhong

Kah

et al. 2020

Preprint

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Understanding heat dissipation processes at nanoscale during cellular thermogenesis is essential to clarify the relationships between the heat and biological processes in cells and organisms. A key parameter determining the heat flux inside a cell is the local thermal conductivity, a factor poorly investigated both experimentally and theoretically. Here, using a nanoheater/nanothermometer hybrid based on a polydopamine shell encapsulating a fluorescent diamond nanocrystal, we measured the intracellular thermal conductivity of HeLa cell with a spatial resolution of about 200 nm. Its mean value of 0.11 Wm -1 K -1 determined for the first time is significantly smaller than that of water. Bayesian analysis of the data strongly supports the existence of variation of the intracellular thermal conductivity of about 40%. These results present a major milestone towards understanding the intracellular heat transfer phenomena at nanoscale.

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Intracellular temperature measurements with fluorescent polymeric thermometers

Cited by 112 publications

References 165 publications

Lanthanide‐Based Thermometers: At the Cutting‐Edge of Luminescence Thermometry

Lanthanide‐Based Thermometers: At the Cutting‐Edge of Luminescence Thermometry

Thermal Properties of Lipid Bilayers Determined Using Upconversion Nanothermometry

In situ measurement of intracellular thermal conductivity using heater-thermometer hybrid diamond nanosensor

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