Intracellular Synthesis of Gold Nanoparticles Using an Ectomycorrhizal Strain EM-1083 of Laccaria fraterna and Its Nanoanti-quorum Sensing Potential Against Pseudomonas aeruginosa

Samanta, Sreeparna; Singh, Braj Raj; Adholeya, Alok

doi:10.1007/s12088-017-0662-4

Cited by 38 publications

(24 citation statements)

References 51 publications

(60 reference statements)

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“…Ali et al [20] also reported similar findings where AgNPs inhibited QS-mediated virulence factors. Our SEM analysis results agreed with previous findings of Samantha et al [42]. They showed that AuNPs, synthesized using fungus Laccaria fraterna, also inhibited biofilm formation in the same manner.…”

Section: Discussionsupporting

confidence: 92%

Biogenic Gold Nanoparticles as Potent Antibacterial and Antibiofilm Nano-Antibiotics against Pseudomonas aeruginosa

et al. 2020

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Plant-based synthesis of eco-friendly nanoparticles has widespread applications in many fields, including medicine. Biofilm—a shield for pathogenic microorganisms—once formed, is difficult to destroy with antibiotics, making the pathogen resistant. Here, we synthesized gold nanoparticles (AuNPs) using the stem of an Ayurvedic medicinal plant, Tinospora cordifolia, and studied the action of AuNPs against Pseudomonas aeruginosa PAO1 biofilm. The synthesized AuNPs were characterized by techniques such as ultraviolet-visible spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, energy-dispersive X-ray diffraction, X-ray diffraction, scanning electron microscopy (SEM), and transmission electron microscopy. The AuNPs were spherically shaped with an average size of 16.1 nm. Further, the subminimum inhibitory concentrations (MICs) of AuNPs (50, 100, and 150 µg/mL) greatly affected the biofilm-forming ability of P. aeruginosa, as observed by crystal violet assay and SEM, which showed a decrease in the number of biofilm-forming cells with increasing AuNP concentration. This was further justified by confocal laser scanning microscopy (CLSM), which showed irregularities in the structure of the biofilm at the sub-MIC of AuNPs. Further, the interaction of AuNPs with PAO1 at the highest sub-MIC (150 µg/mL) showed the internalization of the nanoparticles, probably affecting the tendency of PAO1 to colonize on the surface of the nanoparticles. This study suggests that green-synthesized AuNPs can be used as effective nano-antibiotics against biofilm-related infections caused by P. aeruginosa.

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Section: Discussionsupporting

confidence: 92%

Biogenic Gold Nanoparticles as Potent Antibacterial and Antibiofilm Nano-Antibiotics against Pseudomonas aeruginosa

et al. 2020

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“…To the best of our knowledge, the inhibitory effects of F-AuNPs towards P. aeruginosa virulence factors at both the phenotypic and genetic levels have remained unknown. So far, only AuNPs synthesized from ectomycorrhizal fungi were found to completely inhibit pyocyanin production by P. aeruginosa [74]. Therefore, the finding of anti-virulence activity of F-AuNPs against bacteria, as obtained in the present study, has provided essential insights for the future application of F-AuNPs in controlling P. aeruginosa pathogenesis, as well as against biofilm-related infections.…”

Section: Discussionsupporting

confidence: 58%

Fucoidan-Stabilized Gold Nanoparticle-Mediated Biofilm Inhibition, Attenuation of Virulence and Motility Properties in Pseudomonas aeruginosa PAO1

et al. 2019

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The emergence of antibiotic resistance in Pseudomonas aeruginosa due to biofilm formation has transformed this opportunistic pathogen into a life-threatening one. Biosynthesized nanoparticles are increasingly being recognized as an effective anti-biofilm strategy to counter P. aeruginosa biofilms. In the present study, gold nanoparticles (AuNPs) were biologically synthesized and stabilized using fucoidan, which is an active compound sourced from brown seaweed. Biosynthesized fucoidan-stabilized AuNPs (F-AuNPs) were subjected to characterization using UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), field emission transmission electron microscopy (FE-TEM), dynamic light scattering (DLS), and energy dispersive X-ray diffraction (EDX). The biosynthesized F-AuNPs were then evaluated for their inhibitory effects on P. aeruginosa bacterial growth, biofilm formation, virulence factor production, and bacterial motility. Overall, the activities of F-AuNPs towards P. aeruginosa were varied depending on their concentration. At minimum inhibitory concentration (MIC) (512 µg/mL) and at concentrations above MIC, F-AuNPs exerted antibacterial activity. In contrast, the sub-inhibitory concentration (sub-MIC) levels of F-AuNPs inhibited biofilm formation without affecting bacterial growth, and eradicated matured biofilm. The minimum biofilm inhibition concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were identified as 128 µg/mL. Furthermore, sub-MICs of F-AuNPs also attenuated the production of several important virulence factors and impaired bacterial swarming, swimming, and twitching motilities. Findings from the present study provide important insights into the potential of F-AuNPs as an effective new drug for controlling P. aeruginosa-biofilm-related infections.

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“…The scientific communities have shown their interest in the use of AgNPs due to their broad range of antimicrobial activities (Kim et al, 2007;Lara et al, 2011;Brandt et al, 2012) and the convenience during application for their physico-chemical properties and surface area to volume ratio (Kim et al, 2007; Figure 3). Various other types of NPs, like AuNPs, TiO 2 , SiO 2 , and ZnO, from microbial sources possess the efficacy of inhibiting the QS cascade and thus inhibit the formation of the biofilm (Shah et al, 2008;Samanta et al, 2017;Al-Shabib et al, 2018).…”

Section: Mechanism Of Inhibition Of Quorum Sensing By Micro Biogenic Npsmentioning

confidence: 99%

“…The enzyme also helps in degrading the moiety associated with acyl homoserine lactone and brings about the conformational changes of the signaling molecule, thus preventing the binding with LuxR transcriptional regulator resulting in the inhibition of QS (Kaufmann et al, 2005;Bai et al, 2008). They were also able to inhibit the metabolic activities and production of EPS, preventing the formation of biofilm and changing the hydrophobicity of the bacterial cells (Samanta et al, 2017). The Au NP produced from the mycelium of Laccaria fraternal was responsible for stabilizing the NPs which in turn plays an important role in the reduction of pyocyanin production from P. aeruginosa (Samanta et al, 2017).…”

Section: Inhibition Of Quorum Sensing Using Micro Biogenic Gold Nanopmentioning

confidence: 99%

“…They were also able to inhibit the metabolic activities and production of EPS, preventing the formation of biofilm and changing the hydrophobicity of the bacterial cells (Samanta et al, 2017). The Au NP produced from the mycelium of Laccaria fraternal was responsible for stabilizing the NPs which in turn plays an important role in the reduction of pyocyanin production from P. aeruginosa (Samanta et al, 2017).…”

Section: Inhibition Of Quorum Sensing Using Micro Biogenic Gold Nanopmentioning

confidence: 99%

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Microbiologically-Synthesized Nanoparticles and Their Role in Silencing the Biofilm Signaling Cascade

et al. 2021

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The emergence of bacterial resistance to antibiotics has led to the search for alternate antimicrobial treatment strategies. Engineered nanoparticles (NPs) for efficient penetration into a living system have become more common in the world of health and hygiene. The use of microbial enzymes/proteins as a potential reducing agent for synthesizing NPs has increased rapidly in comparison to physical and chemical methods. It is a fast, environmentally safe, and cost-effective approach. Among the biogenic sources, fungi and bacteria are preferred not only for their ability to produce a higher titer of reductase enzyme to convert the ionic forms into their nano forms, but also for their convenience in cultivating and regulating the size and morphology of the synthesized NPs, which can effectively reduce the cost for large-scale manufacturing. Effective penetration through exopolysaccharides of a biofilm matrix enables the NPs to inhibit the bacterial growth. Biofilm is the consortia of sessile groups of microbial cells that are able to adhere to biotic and abiotic surfaces with the help extracellular polymeric substances and glycocalyx. These biofilms cause various chronic diseases and lead to biofouling on medical devices and implants. The NPs penetrate the biofilm and affect the quorum-sensing gene cascades and thereby hamper the cell-to-cell communication mechanism, which inhibits biofilm synthesis. This review focuses on the microbial nano-techniques that were used to produce various metallic and non-metallic nanoparticles and their “signal jamming effects” to inhibit biofilm formation. Detailed analysis and discussion is given to their interactions with various types of signal molecules and the genes responsible for the development of biofilm.

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Intracellular Synthesis of Gold Nanoparticles Using an Ectomycorrhizal Strain EM-1083 of Laccaria fraterna and Its Nanoanti-quorum Sensing Potential Against Pseudomonas aeruginosa

Cited by 38 publications

References 51 publications

Biogenic Gold Nanoparticles as Potent Antibacterial and Antibiofilm Nano-Antibiotics against Pseudomonas aeruginosa

Biogenic Gold Nanoparticles as Potent Antibacterial and Antibiofilm Nano-Antibiotics against Pseudomonas aeruginosa

Fucoidan-Stabilized Gold Nanoparticle-Mediated Biofilm Inhibition, Attenuation of Virulence and Motility Properties in Pseudomonas aeruginosa PAO1

Microbiologically-Synthesized Nanoparticles and Their Role in Silencing the Biofilm Signaling Cascade

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