Intracellular polyhydroxyalkanoates recovery by cleaner halogen-free methods towards zero emission in the palm oil mill

Mohammadi, Mitra; Hassan, Mohd Ali; Phang, Lai Yee; Shirai, Yoshihito; Man, Hasfalina Che; Ariffin, Hidayah

doi:10.1016/j.jclepro.2012.07.038

Cited by 27 publications

(13 citation statements)

References 36 publications

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“…In contrast, distilled water disruption treatment with wet biomass obtained recovery (80%) and purity (58%) percentages lower than lyophilized biomass. Mohammadi et al [ 174 ], described a higher purity and recovery yield using distilled water disruption with recombinant bacteria instead of wild bacteria. Recombinant bacterial cell wall is thinner than in wild type bacteria, which facilitates the cell wall breaking by osmotic pressure.…”

Section: Pha Recoverymentioning

confidence: 99%

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

et al. 2017

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Sustainable biofuels, biomaterials, and fine chemicals production is a critical matter that research teams around the globe are focusing on nowadays. Polyhydroxyalkanoates represent one of the biomaterials of the future due to their physicochemical properties, biodegradability, and biocompatibility. Designing efficient and economic bioprocesses, combined with the respective social and environmental benefits, has brought together scientists from different backgrounds highlighting the multidisciplinary character of such a venture. In the current review, challenges and opportunities regarding polyhydroxyalkanoate production are presented and discussed, covering key steps of their overall production process by applying pure and mixed culture biotechnology, from raw bioprocess development to downstream processing.

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Section: Pha Recoverymentioning

confidence: 99%

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

et al. 2017

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“…As in the case of halogenated solvents, also for alcohols, the temperature affects the PHA recovery yield and purity ( Mohammadi et al, 2012a ): a combined synergic effect of ethanol and water has been reported by increasing the temperature from 4 to 30°C, since an increasing interaction between water and ethanol improves the cell wall breakage. Water seems to cause the release of cellular proteins and exert an osmotic pressure on the cells that result easily breakable ( Mohammadi et al, 2012b ). Despite alcohols seem to have a narrow range of applicability in the PHA extraction scenario, their use in scaled-up processes would be strongly encouraged: alcohols, in fact, are generally recognized as “recommended” solvents, benign in terms of acute environmental toxicity and the bio-accumulation ( Henderson et al, 2011 ; Prat et al, 2014 ; Prat et al, 2016 ), as well in terms of health issue (butanol, in particular, is granted GRAS by FEMA and synthesizable from renewable resources thus potentially bio-based).…”

Section: Extraction Of Pha From Single Microbial Strainsmentioning

confidence: 99%

Recovery of Polyhydroxyalkanoates From Single and Mixed Microbial Cultures: A Review

Pagliano

Galletti

Samorì

et al. 2021

Front. Bioeng. Biotechnol.

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An overview of the main polyhydroxyalkanoates (PHA) recovery methods is here reported, by considering the kind of PHA-producing bacteria (single bacterial strains or mixed microbial cultures) and the chemico-physical characteristics of the extracted polymer (molecular weight and polydispersity index). Several recovery approaches are presented and categorized in two main strategies: PHA recovery with solvents (halogenated solvents, alkanes, alcohols, esters, carbonates and ketones) and PHA recovery by cellular lysis (with oxidants, acid and alkaline compounds, surfactants and enzymes). Comparative evaluations based on the recovery, purity and molecular weight of the recovered polymers as well as on the potential sustainability of the different approaches are here presented.

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“…Lastly, the type of microorganism and culture conditions can also influence the DSP. Usually PHA-accumulating wild type bacteria have stronger cell walls than recombinant strains [138]. Mixed microbial cultures (MMC) are claimed to be more resistant to cell hydrolysis than pure cultures [44].…”

Section: Factors Affecting the Recovery Of Phasmentioning

confidence: 99%

A sustainable approach for the downstream processing of bacterial polyhydroxyalkanoates: State-of-the-art and latest developments

Pérez-Rivero

López‐Gómez

Roy

2019

Biochemical Engineering Journal

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Bioplastics have emerged as a platform to reduce our dependence on fossil fuels. Polyhydroxyalkanoates (PHAs) are a family of biodegradable polyesters with large potential in consumer goods and medical applications. These polymers accumulate in prokaryotic microbes and their recovery is a challenging, often under explored, operation. In the past, oil-derived solvents and chemicals have been widely used as extracting agents, compromising the "environmentally-friendly" claim of bioplastics. Furthermore, the large amount of chemicals and solvents required at the industrial level would negatively impact the economics of the process. The present review presents the latest advances in the field of downstream operations for PHA recovery emphasizing those green technologies with scaling-up feasibility. As for the upstream and fermentation stages, the extraction process needs to be carefully optimized to accomplish a competitive production of PHAs.

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Intracellular polyhydroxyalkanoates recovery by cleaner halogen-free methods towards zero emission in the palm oil mill

Cited by 27 publications

References 36 publications

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production

Recovery of Polyhydroxyalkanoates From Single and Mixed Microbial Cultures: A Review

A sustainable approach for the downstream processing of bacterial polyhydroxyalkanoates: State-of-the-art and latest developments

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