Intracellular Pathogenesis of Listeriosis

Southwick, Frederick S.; Purich, Daniel L.

doi:10.1056/nejm199603213341206

Cited by 245 publications

(150 citation statements)

References 43 publications

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“…Healthy humans usually clear L. monocytogenes infection with little or no clinical symptoms. However, in patients with predisposing conditions, such as diabetes mellitus, liver failure, HIV infection, immune suppression, splenectomy, older age (> 75 years), and pregnancy, or undergoing anti-TNF-α therapy, listeria can cause a potentially life-threatening disease with clinical symptoms ranging from local inflammatory responses to meningoencephalitis, sepsis with suppurative granulomas in multiple organs, or devastating maternal/fetal infection in pregnant women (13,14). In these patient populations, the incidence is as high as 210 cases per 100,000 (as compared with 0.7 per 100,000 cases in healthy individuals), and mortality can reach 30% (13).…”

Section: Introductionmentioning

confidence: 99%

Indoleamine 2,3-dioxygenase–expressing dendritic cells form suppurative granulomas following Listeria monocytogenes infection

Попов¹,

Abdullah²,

et al. 2006

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Control of pathogens by formation of abscesses and granulomas is a major strategy of the innate immune system, especially when effector mechanisms of adaptive immunity are insufficient. We show in human listeriosis that DCs expressing indoleamine 2,3-dioxygenase (IDO), together with macrophages, are major cellular components of suppurative granulomas in vivo. Induction of IDO by DCs is a cell-autonomous response to Listeria monocytogenes infection and was also observed in other granulomatous infections with intracellular bacteria, such as Bartonella henselae. Reporting on our use of the clinically applied anti-TNF-α antibody infliximab, we further demonstrate in vitro that IDO induction is TNF-α dependent. Repression of IDO therefore might result in exacerbation of granulomatous diseases observed during anti-TNF-α therapy. These findings place IDO + DCs not only at the intersection of innate and adaptive immunity but also at the forefront of bacterial containment in granulomatous infections.

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Section: Introductionmentioning

confidence: 99%

Indoleamine 2,3-dioxygenase–expressing dendritic cells form suppurative granulomas following Listeria monocytogenes infection

Попов¹,

Abdullah²,

et al. 2006

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“…Listeria monocytogenes is an intracellular pathogen that primarily infects APCs and has adapted for life in the cytoplasm of these cells (12,13). Host cells, such as macrophages, actively phagocytose L. monocytogenes, and the majority of the bacteria are degraded in the phagolysosome (14).…”

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confidence: 99%

Fusion to Listeriolysin O and Delivery by Listeria monocytogenes Enhances the Immunogenicity of HER-2/neu and Reveals Subdominant Epitopes in the FVB/N Mouse

Singh

Dominiecki

Jaffee³

et al. 2005

The Journal of Immunology

103

134

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Five overlapping fragments of rat HER-2/neu have been expressed in recombinant Listeria monocytogenes. Each fragment of HER-2/neu is secreted as a fusion protein with a truncated, nonhemolytic form of listeriolysin O (LLO). Lm-LLO-EC1, Lm-LLO-EC2, and Lm-LLO-EC3 overlap the extracellular domain of HER-2/neu, whereas Lm-LLO-IC1 and Lm-LLO-IC2 span the intracellular domain. All five strains controlled the growth of established NT-2 tumors, a rat HER-2/neu-expressing tumor line derived from a spontaneously arising mammary tumor in a FVB/N HER-2/neu-transgenic mouse. The antitumor effect of each of these vaccine constructs was abrogated by the in vivo depletion of CD8+ T cells, although only one known epitope has been defined previously and is present in Lm-LLO-EC2. Anti-HER-2/neu CTL responses were generated by each of the rLm vaccine constructs. With the use of a panel of 3T3 cell lines expressing overlapping fragments of HER-2/neu, regions of HER-2/neu with potential CD8+ T cell epitopes have been defined. DNA vaccines expressing either a fragment or full-length HER-2/neu were constructed in LLO-fused and non-LLO-fused forms. CTL analysis of the DNA vaccines revealed a broadening in the regions of HER-2/neu recognizable as targets when the target Ag is fused to LLO. These studies show the efficacy of L. monocytogenes-based HER-2/neu vaccines in a murine model of breast cancer and also that the immunogenicity of self-Ags can be increased by fusion to LLO and delivery by L. monocytogenes revealing subdominant epitopes.

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“…12 A homozygous recessive mutation causing G M1 gangliosidosis in Shiba dogs has been identified as the deletion of a cytosine residue at nucleotide position 1647 of the putative coding region for canine ␤-galactosidase, 10 which can be detected by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method using purified genomic DNA as a template. 10,11 Hereafter, extensive genotyping studies of specimens from the large, widely distributed population of Shiba dogs are needed to control this canine disease and decrease the incidence rate. For this purpose, it is important that the genotyping test, including collection and delivery of the specimens, should become more rapid and easy.…”

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Eye Infections due toListeria Monocytogenesin Three Cows and One Horse

et al. 2004

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A retrospective study was conducted to determine case histories, microbiological characteristics, and molecular subtypes associated with Listeria monocytogenes infections of the eye in large animals. For selected cases, environmental L. monocytogenes contamination patterns on case farms were also evaluated to probe for potential sources and spread of listerial eye infections. Records of 170 L. monocytogenes isolates from animal infections were reviewed to determine the fraction of isolates associated with eye infections (conjunctivitis, keratitis, and uveitis) of animals and to gather information on the clinical history of these cases. Overall, 4 of 170 Listeria monocytogenes isolates were associated with eye infections; 3 of these had occurred in cows and 1 in a horse. Molecular subtyping (by EcoRI ribotying) showed that 4 different L. monocytogenes subtypes were isolated from these 4 cases; the same ribotypes had previously been found among invasive animal listeriosis infections. Although a variety of L. monocytogenes subtypes were isolated from environmental sources, on 1 farm, the same ribotype associated with the eye infection was also isolated from a fecal sample of a healthy animal and from a soil sample. The data reported in this study further suggest that L. monocytogenes can be a cause of eye infections in several animal species. Listerial eye infections do not seem to require specific pathogen-related virulence characteristics but rather seem to be a function of environmental or host factors, such as direct exposure of the eyes of susceptible animals to high numbers of the pathogen. Although listerial eye infections are rarely diagnosed because of its ubiquitous nature, L. monocytogenes may have to be considered more commonly as a causative agent of eye infections in ruminants and horses.

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Intracellular Pathogenesis of Listeriosis

Cited by 245 publications

References 43 publications

Indoleamine 2,3-dioxygenase–expressing dendritic cells form suppurative granulomas following Listeria monocytogenes infection

Indoleamine 2,3-dioxygenase–expressing dendritic cells form suppurative granulomas following Listeria monocytogenes infection

Fusion to Listeriolysin O and Delivery by Listeria monocytogenes Enhances the Immunogenicity of HER-2/neu and Reveals Subdominant Epitopes in the FVB/N Mouse

Eye Infections due toListeria Monocytogenesin Three Cows and One Horse

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