Intracellular oscillations and release of cyclic AMP from Dictyostelium cells

Gerisch, Günther; Wick, U.

doi:10.1016/s0006-291x(75)80102-1

Cited by 293 publications

(75 citation statements)

References 5 publications

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“…Half-maximal spore induction is obtained with 3 X 10-6 M cAMP (near the physiological range; refs. [18][19][20], whereas 10-3 M ATP, AMP, or adenosine is completely ineffective. Neither ions such as Ca2+ nor any other component of the medium is essential for spore formation.…”

Section: Resultsmentioning

confidence: 99%

cAMP and spore differentiation in Dictyostelium discoideum.

Kay¹

1982

Proc. Natl. Acad. Sci. U.S.A.

114

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The individual amoebae composing a Dictyostelium aggregate can differentiate into either stalk or spore cells according, it is believed, to the extracellular signals they receive. By inducing the differentiation ofisolated cells we hope to identify these signals. It is shown here that wild-type cells can differentiate into prespore cells in a solution of cAMP plus salts supplemented by conditioned medium. Cell-to-cell contact is not required. More important, isolated cells of various sporogenous mutant strains form spores in similar conditions without needing conditioned medium at all. For these strains at least, cAMP is the sole inducer necessary for spore formation. Earlier work has shown that stalk cells are induced by a combination of cAMP and a low molecular weight factor, differentiation inducing factor (DIF). DIF now appears to be the only pathway-specific inducer involved in the differentiation of sporogenous amoebae and DIF levels in the aggregate may therefore determine whether an amoeba becomes a stalk or a spore cell. In suitable conditions some sporogenous mutants form migrating slugs having an anterior/posterior pattern ofprestalk and prespore cells. This pattern could be generated by the localized activity of DIF. (5) where it serves to induce a later group of gene products (6-9). Differentiation in monolayers of cells that are prevented from undergoing normal morphogenesis by, for instance, a restrictive overlay of cellophane has been reported (10)(11)(12). At high cell density cAMP induces the differentiation of stalk and prespore cells but not of mature spores. However, mutants-termed sporogenous-have been selected in which spores also form and these strains have proved very useful in the current work.It has been repeatedly observed that amoebae can only differentiate into spore or prespore cells if they have been in contact with other cells (9)(10)(11)(13)(14)(15). Recently the resulting belief in a cell contact-dependent inductive process has been challenged by the finding that isolated sporogenous amoebae can form spores-in improved incubation conditions-in the presence of cAMP (16). Nonetheless, spore formation still needed unknown soluble factors released by the cells.After further improvement of the incubation conditions, I show here that wild-type prespore cells can also differentiate without cell-to-cell contact and that cAMP alone is a sufficient inducer of spore formation in various sporogenous strains.MATERIALS AND METHODS Cells. Sporogenous mutants HM18 (tsg-900, acr-900, cob-900, sci-907), HM28 (tsg-901 or tsg-903 or both, cyc-900, whi-900, sci-909), and HM29 (tsg-901 or tsg-903 or both, cyc-900, whi-900, sci-910) derive from the wild-type strain V12M2.

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Section: Resultsmentioning

confidence: 99%

cAMP and spore differentiation in Dictyostelium discoideum.

Kay¹

1982

Proc. Natl. Acad. Sci. U.S.A.

114

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“…Previously we have shown that cAMP mediated cGMP accumulation also occurs at physiological concentrations for chemotaxis, that the K a for this response is in the same range as the dissociation constant of the cell-surface bound cAMP receptor and that cGMP elevation precedes pseudopod formation [10]. Contrary to cAMP [23,24], cGMP function and hydrolysis seems to be mainly intracellular. Both cAMP and cGMP phosphodiesterase are present intracellularly.…”

Section: Discussionmentioning

confidence: 95%

Cyclic AMP and folic acid mediated cyclic GMP accumulation in Dictyostelium discoideum

et al. 1977

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“…Real-time cAMP measurements have also revealed complex temporal patterns, including oscillations. cAMP oscillations were first described as an important property of developing Dictyostelium slime moulds (Gerisch and Wick, 1975). These oscillations involve the excretion of cAMP with binding to extracellular cell surface receptors (Maeda et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Role of phosphodiesterases in the shaping of sub-plasma membrane cAMP oscillations and pulsatile insulin secretion

Tian

Sågetorp

et al. 2012

Journal of Cell Science

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SummarySpecificity and versatility in cyclic AMP (cAMP) signalling are governed by the spatial localisation and temporal dynamics of the signal. Phosphodiesterases (PDEs) are important for shaping cAMP signals by hydrolyzing the nucleotide. In pancreatic b-cells, glucose triggers sub-plasma-membrane cAMP oscillations, which are important for insulin secretion, but the mechanisms underlying the oscillations are poorly understood. Here, we investigated the role of different PDEs in the generation of cAMP oscillations by monitoring the concentration of cAMP in the sub-plasma-membrane space ([cAMP]

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Intracellular oscillations and release of cyclic AMP from Dictyostelium cells

Cited by 293 publications

References 5 publications

cAMP and spore differentiation in Dictyostelium discoideum.

cAMP and spore differentiation in Dictyostelium discoideum.

Cyclic AMP and folic acid mediated cyclic GMP accumulation in Dictyostelium discoideum

Role of phosphodiesterases in the shaping of sub-plasma membrane cAMP oscillations and pulsatile insulin secretion

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