Intracellular optical probing with gold nanostars

Spedalieri, Cecilia; Szekeres, Gergő Péter; Werner, Stephan; Kneipp, Janina

doi:10.1039/d0nr07031a

Cited by 21 publications

(46 citation statements)

References 73 publications

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“…Gold(III) chloride trihydrate and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were purchased from Sigma-Aldrich (Steinheim, Germany). Gold nanostars were synthesized using a published protocol [ 42 ] with modifications as described previously [ 23 ]. Briefly, HEPES buffer (pH 7.4) at a concentration of 0.375 M was prepared with Milli-Q water and filtered through a 0.22 µm-pore filter.…”

Section: Methodsmentioning

confidence: 99%

“…The combination of both types of information provides a broader picture of how the different cells process the nanostars. Differently from our previous work on fibroblast and epithelial carcinoma cells, where we have discussed the interaction of gold nanostars with the cells for long incubation times [ 23 ], here we will shed light on the influence of the composition of the biomolecular corona and the nanoparticle morphology, mainly spike length, for shorter incubation times that exclude late stages of endolysosomal maturation. As the data reported here demonstrate, nanostars taken up by 3T3 fibroblast cells, HCT-116 epithelial carcinoma cells, and J774 macrophages have a protein corona of different chemical and structural properties that are a consequence of the specific endolysosomal processing of each individual cell type.…”

Section: Introductionmentioning

confidence: 99%

“…The unique geometry of plasmonic nanostars enables versatile field enhancement [ 11 , 12 ], that allows for efficient metal-enhanced fluorescence [ 13 ] and a high electromagnetic contribution to surface-enhanced Raman scattering (SERS) [ 1 , 14 ]. The latter enables their application not only as SERS tags with reporters for imaging [ 15 , 16 ] and both extracellular [ 17 , 18 ] and intracellular [ 19 , 20 ] sensing, but also as SERS probes of biomolecules [ 21 ] and of the intrinsic biochemistry in living cells [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Specifically, a better understanding is being gained on the surface composition of gold nanostructures in the course of the endolysosomal pathway when they are delivered into the cells through the culture medium [ 32 , 33 , 34 , 35 , 36 ]. We have recently shown that the biomolecular surface composition of gold nanostars can be characterized in living cells, that it varies depending on the morphological properties of the nanostars, and that it leads to different intracellular processing of the nanostars [ 23 ]. Nevertheless, data in this work [ 23 ] were obtained from cell lines that are known to preferentially take up nanoparticles of this size range by clathrin-mediated or calveolar-mediated endocytosis [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

“…We have recently shown that the biomolecular surface composition of gold nanostars can be characterized in living cells, that it varies depending on the morphological properties of the nanostars, and that it leads to different intracellular processing of the nanostars [ 23 ]. Nevertheless, data in this work [ 23 ] were obtained from cell lines that are known to preferentially take up nanoparticles of this size range by clathrin-mediated or calveolar-mediated endocytosis [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

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Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars

et al. 2021

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Gold nanostars are a versatile plasmonic nanomaterial with many applications in bioanalysis. Their interactions with animal cells of three different cell lines are studied here at the molecular and ultrastructural level at an early stage of endolysosomal processing. Using the gold nanostars themselves as substrate for surface-enhanced Raman scattering, their protein corona and the molecules in the endolysosomal environment were characterized. Localization, morphology, and size of the nanostar aggregates in the endolysosomal compartment of the cells were probed by cryo soft-X-ray nanotomography. The processing of the nanostars by macrophages of cell line J774 differed greatly from that in the fibroblast cell line 3T3 and in the epithelial cell line HCT-116, and the structure and composition of the biomolecular corona was found to resemble that of spherical gold nanoparticles in the same cells. Data obtained with gold nanostars of varied morphology indicate that the biomolecular interactions at the surface in vivo are influenced by the spike length, with increased interaction with hydrophobic groups of proteins and lipids for longer spike lengths, and independent of the cell line. The results will support optimized nanostar synthesis and delivery for sensing, imaging, and theranostics.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars

et al. 2021

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Plasmonic Nanostars: Unique Properties That Distinguish Them from Spherical Nanoparticles from a Biosensing Perspective

Tukova,

Nguyen,

Garcia‐Bennett

et al. 2024

Advanced Optical Materials

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Over the past three decades, plasmonic nanostructures, particularly spherical ones, have seen remarkable advancements. Recently, attention has shifted toward anisotropic nanoparticles, especially star‐shaped/branched structures such as plasmonic nanostars (PNS), due to their distinct properties. PNS offers superior electromagnetic enhancement effects, larger surface areas, and as well as non‐linear and unusual photothermal properties, setting them apart from spherical counterparts. Despite significant progress in synthetic methods and characterization of the particles, challenges remain in transitioning PNS technology into practical use. In this perspective article, the distinctive attributes of PNS in biosensing applications are discussed, beginning with an exploration of synthesis methodologies. Their optoelectronic properties are examined and discussed how these properties influence their interaction with different molecules from a biosensing perspective. With a focus on PNS, detailed insights are offered into their unique properties, current applications, and future potential. By fostering discussion and understanding of PNS development, this article aims to facilitate the translation of PNS technology into practical applications, encouraging targeted improvements and advancements.

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In situ formation of alginic acid‐gold nanohybrids for application in cancer photothermal therapy

Figueiredo,

Rodrigues,

Fernandes

et al. 2023

Biotechnology Journal

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Gold‐based nanoparticles present excellent optical properties that propelled their widespread application in biomedicine, from bioimaging to photothermal applications. Nevertheless, commonly employed manufacturing methods for gold‐based nanoparticles require long periods and laborious protocols that reduce cost‐effectiveness and scalability. Herein, a novel methodology was used for producing gold‐alginic acid nanohybrids (Au‐Alg‐NH) with photothermal capabilities. This was accomplished by promoting the in situ reduction and nucleation of gold ions throughout a matrix of alginic acid by using ascorbic acid. The results obtained reveal that the Au‐Alg‐NHs present a uniform size distribution and a spike‐like shape. Moreover, the nanomaterials were capable to mediate a temperature increase of ≈11°C in response to the irradiation with a near‐infrared region (NIR) laser (808 nm,1.7 W cm−2). The in vitro assays showed that Au‐Alg‐NHs were able to perform a NIR light‐triggered ablation of cancer cells (MCF‐7), being observed a reduction in the cell viability to ≈27%. Therefore, the results demonstrate that this novel methodology holds the potential for producing Au‐Alg‐NH with photothermal capacity and higher translatability to the clinical practice, namely for cancer therapy.This article is protected by copyright. All rights reserved

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Intracellular optical probing with gold nanostars

Abstract: The morphology of non-functionalized gold nanostars determines the surface protein structure and their interaction with the endolysosomal compartment in cells.

Cited by 21 publications

References 73 publications

Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars

Probing the Intracellular Bio-Nano Interface in Different Cell Lines with Gold Nanostars

Plasmonic Nanostars: Unique Properties That Distinguish Them from Spherical Nanoparticles from a Biosensing Perspective

In situ formation of alginic acid‐gold nanohybrids for application in cancer photothermal therapy

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