Intracellular microRNA quantification in intact cells: a novel strategy based on reduced graphene oxide-based fluorescence quenching

Paulmurugan, Ramasamy; Ajayan, Pulickel M.; Liepmann, Dorian; Renugopalakrishnan, V.

doi:10.1557/mrc.2018.120

Cited by 3 publications

(4 citation statements)

References 46 publications

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“…Additionally, we verified structural differences in single-stranded and double-stranded RNA–DNA, RNA–RNA, RNA–DNA, and DNA–DNA hybrids by analyzing the sugar–phosphate backbone at 785 cm –1 . The overall findings of the study support the applicability of spontaneous RS for the identification of single-stranded and hybridized miRNA oligomers . Our future efforts will be directed toward evaluating the potential of this technique on clinical samples and designing a multiplex graphene-based biosensor for simultaneous detection of multiple miRNAs.…”

Section: Discussionsupporting

confidence: 61%

Spectrochemical Probing of MicroRNA Duplex Using Spontaneous Raman Spectroscopy for Biosensing Applications

et al. 2020

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MicroRNAs are emerging as both diagnostic and therapeutic targets in different human pathologies. An accurate understanding of the structural dependency of microRNAs for their biological functions is essential for designing synthetic oligos with various base and linkage modifications that can transform into highly sensitive diagnostic devices and therapeutic molecules. In this proof-of-principle study, we have utilized label-free spontaneous Raman spectroscopy to understand the structural differences in sense and antisense microRNA-21 by hybridizing them with complementary RNA and DNA oligos. Overall, the results suggest that the changes in the Raman band at 785 cm–1 originating from the phosphodiester bond of the nucleic acid backbone, linking 5′ phosphate of the nucleic acid with 3′ OH of the other nucleotide, can serve as a marker to identify these structural variations. Our results support the application of Raman spectroscopy in discerning intramolecular (ssRNA and ssDNA) and intermolecular (RNA–RNA, RNA–DNA, and DNA–DNA hybrids) interactions of nucleic acids. This is potentially useful for developing biosensors to quantify microRNAs in clinical samples and to design therapeutic microRNAs with robust functionality.

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Section: Discussionsupporting

confidence: 61%

Spectrochemical Probing of MicroRNA Duplex Using Spontaneous Raman Spectroscopy for Biosensing Applications

et al. 2020

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“…29 The obtained results confirmed already published data, where GOantisense miRNA-21 complex targeting miRNA-21 was found to effectively reduce the expression of miRNA-21 in cancer cells and inhibit their proliferation and migration. 30,31 Moreover, our studies showed that GO-antisense miRNA-21 could modify the inflammatory response by affecting the cytokine expression level. IL-6, as an inflammatory cytokine, regulates the inflammatory reaction and increased cell proliferation and angiogenesis in cancer cells.…”

mentioning

confidence: 78%

“…28 GO has been shown to regulate cytokine protein expression 29 in glioblastoma by delivering therapeutic agents that target specific cytokines or cytokine pathways. 30 GO in glioma cells also regulated the protein expression level of IL-6, IL-8, and MCP-1 by the activation of the NF-κB signaling pathway. 31 The downregulation of IL-6 and Il-8 was also correlated with decreased levels of angiogenesis in glioma in an in ovo cancer model.…”

Section: Dovepressmentioning

confidence: 92%

Influence of GO-Antisense miRNA-21 on the Expression of Selected Cytokines at Glioblastoma Cell Lines

Kutwin,

Sosnowska,

Ostrowska

et al. 2023

IJN

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Introduction: Graphene oxide (GO) is a single layer of carbon atoms with unique properties, which are beneficial due to its surface functionalisation by miRNA. miRNAs are a non-coding small form of RNA that suppress the expression of protein-coding genes by translational repression or degradation of messenger RNA. Antisense miRNA-21 is very promising for future investigation in cancer therapy. This study aimed to detect cytokine expression levels after the administration of GO-antisense miRNA-21 into U87, U118, U251 and T98 glioma cell lines. Methods: U87, U118, U251 and T98 glioma cell line were investigated in term of viability, human cytokine expression level at protein and genes after treatment with GO, GO-antisense miRNA-21 and antisense miRNA-21. The delivery of antisense miRNA-21 into the glioma cell at in vitro investigation were conducted by GO based transfection and electroporation. Results:The results of the protein microarray and gene expression profile showed that complexes of GO-antisense miRNA-21 modified the metallopeptidase inhibitor 2 (TIMP-2), interleukin-6 (IL-6), interleukin 8 (IL-8), intercellular adhesion molecule 1 (ICAM-1), and monocyte chemoattractant protein-1 (MCP-1) expression level compared to transfection by electroporation of antisense miRNA-21 at investigated glioblastoma cell lines. The TIMP-2 protein and gene expression level was upregulated after antisense miRNA-21 delivery by GO complex into U87, U251 and T98 glioblastoma cell lines comparing to the non-treated control group. The downregulation at protein expression level of ICAM -1 was observed at U87, U118, U251 and T98 glioma cell lines. Moreover, the IL-8 expression level at mRNA for genes and protein was decreased significantly after delivery the antisense-miRNA-21 by GO compared to electroporation as a transfection method. Discussion: This work demonstrated that the graphene oxide complexes with antisense miRNA-21 can effectively modulate the cytokine mRNA and protein expression level at U87, U118, U251 and T98 glioma cell lines.

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“…[45][46][47][48]77 Body-on-a-chip devices coupled with pharmacokinetics models aim to mimic the physiological complexity of inter-organ interactions that might be used to observe continuous or linked pharmacokinetic processes such as ADME (absorption, distribution, excretion, metabolism) of various drug administration routes, and the data obtained may be applied to construct mathematical models for prediction of drug efficacy. [78][79][80] Although the "multi-organ-on-a-chip" concept remains in its infancy, major breakthroughs have been made which includes the design of two-organs 81,82 , three-organs 83,84 , four-organs 46,85 , and tenorgans on the chip. 86…”

Section: Body-on-a-chipmentioning

confidence: 99%

“Organs on a Chip”: Revolutionization in personalized treatment

Sahoo¹,

Patel²,

Goswami³

2021

J. Drug Delivery Ther.

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“Organs-on-a-chip” (OOAC), involves microfluidics based biomaterial sciences, bio-engineering and cell biology majorly cell isolation and cell culturing aspects. This technology claims to develop 3-dimensional tissues structurally and physiologically in simulation to in vivo providing relevant results in terms of physiological and genetic aspects with virtue of its origin from human systems. In recent times, experts from diversified disciplines have developed and established many OOAC systems with an assertion of being perfect for drug research replacing convectional cell cultures and animal testing due to the technical limitations in the applicability of the same in vivo for systemic complexities and genetic variances. “Organs-on-a-chip” has attracted substantial interest for its wide range of applications in fields of drug research, regenerative medicine and personalized medicine. Successful development and establishments of different OOACs will contribute towards newer avenues in the path of precised personalized medicine. Keywords: Organs on a chip, personalized medicine, OOAC, 3D cell culture

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Intracellular microRNA quantification in intact cells: a novel strategy based on reduced graphene oxide-based fluorescence quenching

Cited by 3 publications

References 46 publications

Spectrochemical Probing of MicroRNA Duplex Using Spontaneous Raman Spectroscopy for Biosensing Applications

Spectrochemical Probing of MicroRNA Duplex Using Spontaneous Raman Spectroscopy for Biosensing Applications

Influence of GO-Antisense miRNA-21 on the Expression of Selected Cytokines at Glioblastoma Cell Lines

“Organs on a Chip”: Revolutionization in personalized treatment

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