Intracellular mechanisms coupled to NPY Y2 and Y5 receptor activation and lipid accumulation in murine adipocytes

Dose-dependent effects of neuropeptide Y on the regulation of preadipocyte proliferation and adipocyte lipid synthesis <i>via</i> the PPAR&gamma; pathways

“…Although the stimulatory role of NPY on preadipocyte proliferation has already been reported [8,20,21], the relative effects of different concentrations of NPY is unclear. Relatively high concentrations of NPY had been observed to promote preadipocyte proliferation [21], and NPY stimulated proliferation has been observed in 3T3-L1 cells and rat primary preadipocytes in a concentration-dependent manner: NPY at concentration lower than 10 -9 M had no stimulatory effect, proliferation stimulation was observed at 10 -9 M and reached a maximum at 2×10 -8 M [8]. However, the stimulatory effect was not always observed at these NPY concentrations when treated in co-culture of endothelial cells and preadipocytes with neuroblastoma cells [20].…”

“…NPY has a stimulatory effect on the proliferation of non-adipose cells [14][15][16][17][18][19]; however, studies of NPY on preadipocyte proliferation have provided conflicting results as different concentrations of NPY appear to act through different receptors [8,20,21]. The promotion of adipocyte differentiation by NPY is currently debated in the literature [20,21], some reports have found no significant role for NPY in lipid accumulation [8]. Given that there were several discrepancies in previous study, we focus on the effect of various concentration of NPY on adipogenesis of 3T3-L1 cells in present study.…”

“…NPY-induced lipid accumulation involves increased PPARγ expression and the inhibition of lipolysis, through the Mitogen-Activated Protein Kinase (MAPK), Protein Kinase A (PKA), Protein Kinase C (PKC) and PI3K pathways, by activating the receptors NPY Y1, NPY Y2 and NPY Y5 [21]. However, little is known about the effects of different NPY concentrations on the phosphorylation of these proteins.…”

Neuropeptide Y promotes adipogenic differentiation in primary cultured human adipose-derived stem cells

“…Rosmaninho-Salgado and colleagues [26] showed that NPY activates Y2 and Y5 receptors, thereby inducing PPAR-γ expression and lipid accumulation in 3T3-L1 cells. Rosmaninho-Salgado et al [26] found that NPY could stimulate 3T3-L1 proliferation by Y2 and Y5 receptors, and the antagonists of Y2 and Y5 receptors could inhibit 3T3-L1 proliferation.…”

“…Rosmaninho-Salgado et al [26] found that NPY could stimulate 3T3-L1 proliferation by Y2 and Y5 receptors, and the antagonists of Y2 and Y5 receptors could inhibit 3T3-L1 proliferation. Our current study gives insights for the first time to show that the Y2 and Y5 receptors are expressed in human ADSCs, and NPY may play its role in hADSCs proliferation and adipogenic differentiation through the two receptors.…”