Intracellular location of NADP+-linked malic enzyme in C3 plants

El-Shora, Hamed M.; T, Ap Rees

doi:10.1007/bf00201057

Cited by 26 publications

(12 citation statements)

References 20 publications

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“…Taken together, these facts would seem to indicate that the product of Me2 is a "housekeeping enzyme" that is required for some nonphotosynthetic process in both C, and C, plants. This enzyme probably corresponds to the plastid-localized NADP-ME activity measured in the C, plants marrow and soybean by El-Shora and ap Rees (1991). M e l , on the other hand, appears to be strictly involved with C, photosynthesis, since its expression leve1 correlates with C, photosynthesis in Flaveria, and no expression is seen in roots.…”

Section: Discussionmentioning

confidence: 67%

Two Genes Encode Highly Similar Chloroplastic NADP-Malic Enzymes in Flaveria (Implications for the Evolution of C4 Photosynthesis)

1996

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To gain an understanding of the molecular events underlying the evolution of C, photosynthesis, we have undertaken a detailed study of the NADP-malic enzyme gene family in C, and C, species of Haveria. Three genomic clones from the C, species Haveria bidentis were characterized and found to encode two highly similar chloroplastic forms of NADP-malic enzyme, termed ME1 and ME2. Genomic Southern blotting with gene-specific probes showed that both Me7 and Me2 are found in Haveria trinervia (C,) and fiaveria pringlei (C,) as well as in F. bidentis. Northern blots demonstrated that M e l expression in leaves parallels the degree of C, photosynthesis in seven Haveria species. Furthermore, whereas M e 2 was expressed at a low level in both roots and leaves of f . bidentis, M e l expression was seen only in leaves and was light-regulated. We discuss these results in the context of the evolution of C, photosynthesis in Flaveria.The C, photosynthetic pathway is a recently evolved modification of the C, pathway in which plants spatially separate the initial fixation and subsequent conversion of CO, to 3-phosphoglycerate and other carbohydrates. Through the use of this system, C, plants effectively concentrate CO, in the vicinity of Rubisco (located in bundle-sheath cells) and, thus, avoid the wasteful process of photorespiration. The efficient operation of C, photosynthesis requires the strict compartmentation of a suite of enzymes in either mesophyll or bundle-sheath cells. This intercellular compartmentation is a distinguishing feature of C, plants: a11 of the key enzymes utilized in C, photosynthesis are present in C, plants; however, their expression patterns are distinct from those seen in C, plants. It is generally accepted that C, plants evolved from C, ancestors and that the specific enzymes utilized in C, photosynthesis derived from corresponding enzymes present in the C, ancestors (Cockburn, 1983). In the evolution of C, photosynthesis, the expression programs of these ancestral proteins were Present address:

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Section: Discussionmentioning

confidence: 67%

Two Genes Encode Highly Similar Chloroplastic NADP-Malic Enzymes in Flaveria (Implications for the Evolution of C4 Photosynthesis)

1996

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“…Seeds were germinated according to El-Shora and ap Rees [15]. Seeds of C. pepo were surface sterilized in 10% sodium hypochlorite for 10 min, soaked in running tap-water for 24 h, and then germinated between paper towels, moistened with distilled water in sterilized plastic trays.…”

Section: Seed Germination and Growth Conditionsmentioning

confidence: 99%

Plant growth regulators induced urease activity inCucurbita pepoL. cotyledons

Shora

Ali

2016

Acta Biologica Hungarica

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This study is aimed to investigate the activity of urease (EC 3.5.1.5, urea amidohydrolase) that catalyzes the hydrolysis of urea in 5-day-old Cucurbita pepo cotyledons subjected to various concentrations of different growth regulators. The treatment of C. pepo cotyledons with different concentrations (100-600 µmol) of different auxins [indole-3-acetic acid (IAA), indole butyric acid (IBA), indole propionic acid (IPA) and naphthalene acetic acid (NAA)]; or with different concentrations (100-300 µmol) of different cytokinins [kinetin, zeatin and benzyladenine (6-BA)] resulted in a significant increase of urease activity, compared to control. The optimal effects were recorded for each of 500 µmol of IAA and 300 µmol of zeatin treatments. A gradual increase in urease activity was detected in cotyledons treated with various concentrations (0.2-1.0 mM) of 28-homobrassinolide (HBL), in relative to control. A substantial increase in urease activity was observed in cotyledons subjected to different concentrations of triazole (10-60 mg L -1 ), containing either triadimefon (TDM) or hexaconazole (HEX), compared to control. The combination of 300 µmol zeatin with any of protein inhibitors, namely 5-fluorouridine (FUrd), cordycepin and α-amanitin, resulted in the alleviation of their inhibitory effect on the urease activity.

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“…Maize is the only C 4 plant known to be cultivated in this area [57,87,93]. Other crops grown by the ancient Maya, such as Phaseolus vulgaris L. [31], Cucurbita moschata [88], Capsicum annuum [88], Manihot esculenta [16], Gossypium hirsutum [26], Ipomoea batatas [44], Persea americana [32], Theobroma cacao [26], Anacardium occidentale [4], Psidium guajava [73], Carica papaya [51], and Celtis sp. [53], have been identified as C 3 plants.…”

Section: Introductionmentioning

confidence: 99%