Intracellular glutathione in the peripheral blood cells of HIV-infected patients: failure to show a deficiency

Pirmohamed, Munir; Williams, DE; Tingle, Malcolm D.; Barry, Michael; Khoo, Soo Hean Gary; O'Mahony, Conor; Wilkins, E.G.L.; Breckenridge, A; Park, B. K.

doi:10.1097/00002030-199605000-00008

Cited by 59 publications

(29 citation statements)

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“…However, the usefulness of GSH and, particularly, of GSSG and PSSG as powerful biomarkers of oxidative stress is still hampered by some methodologic artifacts, as highlighted by 2 findings: (a) measurements by different research groups of the concentrations of GSH and, even more, of GSSG and PSSG span a more than 100-fold range in healthy persons (8 ); and (b) in individuals with the same pathology, GSH, GSSG, and PSSG have frequently been observed to increase by one research group and decrease by another group, e.g., in diabetes (23,24 ) or HIV (25,26 ).…”

Section: Discussionmentioning

confidence: 99%

Oxidized Forms of Glutathione in Peripheral Blood as Biomarkers of Oxidative Stress

et al. 2006

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Background: Reduced glutathione (GSH) and its redox forms, glutathione disulfide (GSSG) and glutathionylated proteins (PSSG), are biomarkers of oxidative stress, but methodologic artifacts can interfere with their measurement. We evaluated the importance of correct sample handling during the preanalytical phase for GSH, GSSG, and PSSG measurement. Methods: We used human blood for in vitro experiments with oxidants [tert-butylhydroperoxide (t-BOOH), diamide, and menadione]. For in vivo experiments, we used rats in which we cannulated the jugular and femoral veins for both oxidant administration and blood collection. We measured GSH, GSSG, and PSSG with HPLC with or without sample pretreatment with N-ethylmaleimide (NEM) to prevent artifacts. We also measured malondialdehyde (MDA) with HPLC, and protein carbonyls (PCO) with spectrophotometric procedures. Results: When methodologic artifacts were prevented by pretreatment with NEM, GSSG results increased up to 3-fold over the basal concentrations, even in the presence of 5 mol/L t-BOOH or diamide and 20 mol/L menadione. PSSG increased by ϳ50% at 20 mol/L t-BOOH or diamide and at 50 mol/L menadione. PCO and MDA remained unchanged. In vivo oxidation treatments elicited immediate and significant increases in GSSG and PSSG over basal values (up to 200-fold), whereas PCO and MDA showed only slight variation 120 or 180 min after treatment. Conclusions: With the use of artifact-free measurement methods, GSH, GSSG, and PSSG are potentially powerful and reliable biomarkers of oxidative stress status

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Section: Discussionmentioning

confidence: 99%

Oxidized Forms of Glutathione in Peripheral Blood as Biomarkers of Oxidative Stress

et al. 2006

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“…Elevated plasma or urinary levels of cysteinylglycine are observed in patients with rheumatoid arthritis [27,28] and may be associated with the extend of inflammation. Decrease of glutathione concentration may be associated with aging [29] and the pathogenesis of many diseases, including AIDS [30], Alzheimer's disease [31], alcoholic liver disease [32] and pulmonary diseases [33,34], e.g. chronic obstructive pulmonary disease and asthma.…”

Section: Endogenous Thiolsmentioning

confidence: 99%

Ultraviolet derivatization of low-molecular-mass thiols for high performance liquid chromatography and capillary electrophoresis analysis

Kuśmierek

Chwatko

Głowacki

et al. 2011

Journal of Chromatography B

111

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“…Glutathione plays an essential role in protecting cells from toxic species, such as free radicals and reactive oxygen intermediates [8]. It has been documented that a decrease of glutathione concentration may be associated with aging [9] and the pathogenesis of many diseases, including AIDS [10], Alzheimer's disease [11], alcoholic liver disease [12] and pulmonary diseases [13,14], e.g. chronic obstructive pulmonary disease and asthma.…”

Section: Introductionmentioning

confidence: 99%

Determination of endogenous thiols and thiol drugs in urine by HPLC with ultraviolet detection

Kuśmierek

Chwatko

Głowacki

et al. 2009

Journal of Chromatography B

123

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Intracellular glutathione in the peripheral blood cells of HIV-infected patients: failure to show a deficiency

Cited by 59 publications

References 0 publications

Oxidized Forms of Glutathione in Peripheral Blood as Biomarkers of Oxidative Stress

Oxidized Forms of Glutathione in Peripheral Blood as Biomarkers of Oxidative Stress

Ultraviolet derivatization of low-molecular-mass thiols for high performance liquid chromatography and capillary electrophoresis analysis

Determination of endogenous thiols and thiol drugs in urine by HPLC with ultraviolet detection

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