1999
DOI: 10.1016/s0024-3205(99)00247-7
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Intracellular generation of MDA-lys epitope in foam cells

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Cited by 10 publications
(8 citation statements)
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“…Our results demonstrate that the internalization of cholesterol – without oxidative modification – by macrophages is sufficient to trigger cellular reactions leading to the generation of superoxide. We have reported previously that, upon internalization, ac‐LDL generates the MDA‐lysine epitope in foam cells [41]. It is plausible to suggest that cholesterol loading induces superoxide production, lipid peroxidation, and subsequent generation of MDA‐modification to proteins.…”
Section: Resultsmentioning
confidence: 93%
See 1 more Smart Citation
“…Our results demonstrate that the internalization of cholesterol – without oxidative modification – by macrophages is sufficient to trigger cellular reactions leading to the generation of superoxide. We have reported previously that, upon internalization, ac‐LDL generates the MDA‐lysine epitope in foam cells [41]. It is plausible to suggest that cholesterol loading induces superoxide production, lipid peroxidation, and subsequent generation of MDA‐modification to proteins.…”
Section: Resultsmentioning
confidence: 93%
“…The non‐adherent cells were removed and the monolayers were then placed in DMEM containing 10% fetal bovine serum and supplemented with 100 μg/ml ox‐LDL or ac‐LDL. Foam cell formation was monitored by Oil Red O staining [41]. Monolayers of macrophages on glass cover slips were fixed with 10% formaldehyde in PBS (pH 7.4) for 10 min at room temperature, stained with Oil Red O, counterstained for 10 min with hematoxylin, and then examined under a microscope.…”
Section: Methodsmentioning
confidence: 99%
“…Seven days following the final booster, blood was collected in 0.1% EDTA and plasma was obtained. The titer of this antibody was over 1:20,000 as judged by an ELISA using a method previously described (Huang et al, 1999).…”
Section: Preparation Of Antiserum Against Lgmentioning
confidence: 99%
“…The biological functions of MDA-Lys [N ε -(2-propenal) lysine] adducts generated with proteins have not been well studied. MDA-Lys epitopes are, however, closely associated with atherogenesis (1,2,4) and recognized as markers of oxidation. Oxidative stress is implicated in the pathogenesis of numerous diseases, including diabetes and atherosclerosis.…”
mentioning
confidence: 99%
“…They form Schiff-base adducts with Lys residues and cross-links with proteins in vitro and in vivo. HNE also reacts with Lys residues, primarily via Michael addition reaction (1)(2)(3)(4). These lipid-protein adducts are termed ALEs.…”
mentioning
confidence: 99%