Intracellular free calcium concentration in human taste bud cells increases in response to taste stimuli

Fujiyama, Rie; Miyazaki, Toshihiro; Miyamoto, Terumasa; Okada, Yukio; Mizuno, Akio; Inokuchi, Tsugio; Sato, Toshihide

doi:10.1016/s0014-5793(98)00949-1

Cited by 7 publications

(3 citation statements)

References 17 publications

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“…Taste buds contain different types of receptor cells that sense various tastes, such as bitter, sweet, sour, salty, and umami [3], [4]. Taste receptors type 2 (TAS2R) are responsible for detecting bitter sensation [5].…”

Section: Introductionmentioning

confidence: 99%

Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle

et al. 2014

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Bitter tastants can induce relaxation in precontracted airway smooth muscle by activating big-conductance potassium channels (BKs) or by inactivating voltage-dependent L-type Ca2+ channels (VDLCCs). In this study, a new pathway for bitter tastant-induced relaxation was defined and investigated. We found nifedipine-insensitive and bitter tastant chloroquine-sensitive relaxation in epithelium-denuded mouse tracheal rings (TRs) precontracted with acetylcholine (ACH). In the presence of nifedipine (10 µM), ACH induced cytosolic Ca2+ elevation and cell shortening in single airway smooth muscle cells (ASMCs), and these changes were inhibited by chloroquine. In TRs, ACH triggered a transient contraction under Ca2+-free conditions, and, following a restoration of Ca2+, a strong contraction occurred, which was inhibited by chloroquine. Moreover, the ACH-activated whole-cell and single channel currents of non-selective cation channels (NSCCs) were blocked by chloroquine. Pyrazole 3 (Pyr3), an inhibitor of transient receptor potential C3 (TRPC3) channels, partially inhibited ACH-induced contraction, intracellular Ca2+ elevation, and NSCC currents. These results demonstrate that NSCCs play a role in bitter tastant-induced relaxation in precontracted airway smooth muscle.

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Section: Introductionmentioning

confidence: 99%

Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle

et al. 2014

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“…Electrophysiological results on taste cells, however, have raised questions because the recordings require invasive procedures, may involve cell damage, and often yield relatively small sample sizes (for a discussion see Herness, 2000). Several researchers have used functional imaging on isolated taste cells, isolated taste buds or stripped epithelial sheets to overcome these problems (Akabas et al 1988; Bernhardt et al 1996; Hayashi et al 1996; Ogura et al 1997; Fujiyama et al 1998; Lyall et al 2001; Liu & Simon, 2001; Ohtubo et al 2001), but cellular resolution of signals and restriction of chemical stimulation to the apical chemosensitive tip of taste cells have been serious challenges until now. Here, we have used functional Ca 2+ imaging with laser scanning confocal microscopy in a relatively intact preparation of taste buds to investigate how taste quality is detected.…”

mentioning

confidence: 99%

Individual mouse taste cells respond to multiple chemical stimuli

Caicedo

Kim

Roper

2002

The Journal of Physiology

121

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Sensory organs are specialized to detect and decode stimuli in terms of intensity and quality. In the gustatory system, the process of identifying and distinguishing taste qualities (e.g. bitter versus sweet) begins in taste buds. A central question in gustatory research is how information about taste quality is extracted by taste receptor cells. For instance, whether and how individual taste cells respond to multiple chemical stimuli is still a matter for debate. A recent study showed that taste cells expressing bitter-responsive taste receptors do not also express sweet-responsive taste receptors and vice versa. These results suggest that the gustatory system may use separate cellular pathways to process bitter and sweet signals independently. Results from electrophysiological studies, however, reveal that individual taste receptor cells respond to stimuli representing multiple taste qualities. Here we used non-invasive Ca 2+ imaging in slices of lingual tissue containing taste buds to address the issue of quality detection in murine taste receptor cells. We recorded calcium transients elicited by chemical stimuli representing different taste qualities (sweet, salty, sour and bitter). Many receptor cells (38 %) responded to multiple taste qualities, with some taste cells responding to both appetitive ('sweet') and aversive ('bitter') stimuli. Thus, there appears to be no strict and separate detection of taste qualities by distinct subpopulations of taste cells in peripheral gustatory sensory organs in mice.

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“…Essentially this im plies th a t some cells in one taste bud may respond to one stim ulus, w h ile o th e r cells in the same taste bud may respond to o ther stim uli. Na+ (Fujiyama, 1998;Gilbertson e t al., 2000).…”

Section: 3 4 O Lfa C Tio N H E a Lth A N D Agementioning

confidence: 99%

The concept of flavor styles to classify flavors

Klosse¹

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Intracellular free calcium concentration in human taste bud cells increases in response to taste stimuli

Cited by 7 publications

References 17 publications

Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle

Non-Selective Cation Channels Mediate Chloroquine-Induced Relaxation in Precontracted Mouse Airway Smooth Muscle

Individual mouse taste cells respond to multiple chemical stimuli

The concept of flavor styles to classify flavors

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