Intracellular free Ca<sup>2+</sup> dynamic changes to histamine are reduced in cystic fibrosis human tracheal gland cells

Jacquot, Jacky; Maizières, Michaël; Spilmont, Christophe; Millot, Jean-Marc; Sebille, Stéphane; Merten, Marc; Kammouni, Wafa; Manfait, Michel

doi:10.1016/0014-5793(96)00405-x

Cited by 8 publications

(3 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…38 Furthermore, an abnormal CFTR protein accumulation in the ER of CF gland secretory cells might result in a reduced intracellular Ca 2ϩ handling associated with a defect in the process of exocytosis. 39 The amplitude and the duration of intracellular Ca 2ϩ signals were recently shown to be directly responsible for the differential activation of several Ca 2ϩ -sensitive transcriptional regulators, including the transcription nuclear factor-B (NF-B), c-JunN-terminal kinase (JNK), and NFAT, which are required for the transcriptional activation of diverse pro-inflammatory cytokines, including IL-8. 40 In CF gland cells, a possible mechanism of action could involve the effect of the accumulation of mutant CFTR protein in the ER 41,42 on the synthesis and secretion of cytokines, as overload of the ER by resident proteins activates NF-B by causing a rapid Ca 2ϩ release from the ER.…”

Section: Mmol/l) and Intermediate (135 Mmol/l) CLmentioning

confidence: 99%

Selective Up-Regulation of Chemokine IL-8 Expression in Cystic Fibrosis Bronchial Gland Cells in Vivo and in Vitro

Tabary

Zahm

Hinnrasky

et al. 1998

The American Journal of Pathology

Self Cite

153

123

View full text Add to dashboard Cite

Accumulating evidence suggests that the early pulmonary inflammation pathogenesis in cystic fibrosis (CF) may be associated with an abnormal increase in the production of pro-inflammatory cytokines in the CF lung, even in the absence of infectious stimuli. We have postulated that if baseline abnormalities in airway epithelial cell production of cytokines occur in CF, they should be manifested in the CF bronchial submucosal glands, which are known to express high levels of CFTR (cystic fibrosis transmembrane conductance regulator) protein, the gene product mutated in CF disease. Immunohistochemical analyses showed that CF bronchial submucosal glands in patients homozygous for the deltaF508 deletion expressed elevated levels of the endogenous chemokine interleukin (IL)-8 but not the pro-inflammatory cytokines IL-1beta and IL-6, compared with non-CF bronchial glands. Moreover, basal protein and mRNA expression of IL-8 were constitutively up-regulated in cultured deltaF508 homozygous CF human bronchial gland cells, in an unstimulated state, compared with non-CF bronchial gland cells. Furthermore, the exposure of CF and non-CF bronchial gland cells to an elevated extracellular Cl- concentration markedly increased the release of IL-8, which can be corrected in CF gland cells by reducing the extracellular Cl- concentration. We also found that, in contrast to non-CF gland cells, dexamethasone did not inhibit the release of IL-8 by cultured CF gland cells. The selective up-regulation of bronchial submucosal gland IL-8 could represent a primary event that initiates early airway submucosal inflammation in CF patients. These findings are relevant to the pathogenesis of CF and suggest a novel pathophysiological concept for the early and sustained airway inflammation in CF patients.

show abstract

Section: Mmol/l) and Intermediate (135 Mmol/l) CLmentioning

confidence: 99%

Selective Up-Regulation of Chemokine IL-8 Expression in Cystic Fibrosis Bronchial Gland Cells in Vivo and in Vitro

Tabary

Zahm

Hinnrasky

et al. 1998

The American Journal of Pathology

Self Cite

153

123

View full text Add to dashboard Cite

show abstract

“…Peak Ca 2+ release induced by IL-8 was decreased in CF ASM cells compared to control cells, an observation that was largely explained by a lower resting [Ca 2+ ] i . A similar difference in Ca 2+ regulation in response to histamine has been observed in tracheal gland cells and in nasal epithelial cells of CF patients but the reason for this abnormality was reported as unknown [ 29 , 30 ]. Despite these alterations, neither migration nor proliferation was significantly different between the two groups.…”

Section: Discussionmentioning

confidence: 57%

The effects of interleukin-8 on airway smooth muscle contraction in cystic fibrosis

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Indeed, information such as spectral shifts or spectral shape changes related to molecular interactions, cannot be obtained. In order to take advantage of emission spectroscopy, several microspectrometers using either fluorescence or Raman scattering have been developed [11][12][13][14] and fruitfully used in molecular approaches within living cells such as drugtarget interactions, [15][16][17][18][19] xenobiotics kinetics, 20 -22 ion concentration determinations, [23][24][25] or in situ conformation of macromolecules. 26,27 Most of the microspectrofluorometers built up to now and used for biological studies such as those reported above allow the recording of the spectrum from localized illumination.…”

Section: Introductionmentioning

confidence: 99%

A new UV-visible confocal laser scanning microspectrofluorometer designed for spectral cellular imaging

Favard

Valisa²,

Egret‐Charlier

et al. 1999

Biospectroscopy

Self Cite

View full text Add to dashboard Cite

With the aim to perform spectroscopic studies and spectral images inside living cells, a microspectrofluorometer has been designed for two‐dimensional spectral imaging in the visible and in the near‐UV region. The main advantage of the device relies on its ability to scan the laser beam along one direction of the sample. This scanning is optically coupled with one direction of a bidimensional detector, allowing an instantaneous recording of a one‐dimensional spectral image. The overall scanning of the sample is achieved by means of submicrometric displacements of the stage in the perpendicular direction. The main characteristics and performances of the microspectrofluorometer in terms of sensitivity (detection of a few molecules), spatial resolution (0.5 × 0.5 × 1 μm), and spectral resolution (1 nm) are presented. Finally, applications of this new apparatus concerning in situ localization and spectral characterization of two dyes are shown with Drosophila salivary glands (ethidium bromide) and T47D tumor cells (Hoechst 33342). © 1999 John Wiley & Sons, Inc. Biospectroscopy 5: 101–115, 1999

show abstract

Intracellular free Ca²⁺ dynamic changes to histamine are reduced in cystic fibrosis human tracheal gland cells

Cited by 8 publications

References 34 publications

Selective Up-Regulation of Chemokine IL-8 Expression in Cystic Fibrosis Bronchial Gland Cells in Vivo and in Vitro

Selective Up-Regulation of Chemokine IL-8 Expression in Cystic Fibrosis Bronchial Gland Cells in Vivo and in Vitro

The effects of interleukin-8 on airway smooth muscle contraction in cystic fibrosis

A new UV-visible confocal laser scanning microspectrofluorometer designed for spectral cellular imaging

Contact Info

Product

Resources

About

Intracellular free Ca2+ dynamic changes to histamine are reduced in cystic fibrosis human tracheal gland cells

Cited by 8 publications

References 34 publications

Selective Up-Regulation of Chemokine IL-8 Expression in Cystic Fibrosis Bronchial Gland Cells in Vivo and in Vitro

Selective Up-Regulation of Chemokine IL-8 Expression in Cystic Fibrosis Bronchial Gland Cells in Vivo and in Vitro

The effects of interleukin-8 on airway smooth muscle contraction in cystic fibrosis

A new UV-visible confocal laser scanning microspectrofluorometer designed for spectral cellular imaging

Contact Info

Product

Resources

About

Intracellular free Ca²⁺ dynamic changes to histamine are reduced in cystic fibrosis human tracheal gland cells