Intracellular formation of collagen microfibrils in granulation tissue

Ina, Keisuke; Kitamura, Hirokazu; Tatsukawa, Shuji; Miyazaki, Takashi; Abe, Hirokazu; Fujikura, Yoshihisa

doi:10.1016/j.yexmp.2005.08.003

Cited by 7 publications

(3 citation statements)

References 20 publications

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“…Collagen microfibrils are then formed when regular cross-linking of tropocollagens occur in the extracellular space [ 64 ]. It is known that a regular cross-striated structure characterizes collagen microfibrils at TEM [ 65 ], as we could observe in our findings. Collagen microfibrils were not present in Matrigel, 2D culture and in SH.…”

Section: Discussionsupporting

confidence: 89%

Bottlenose dolphin (Tursiops truncatus) immortalized fibroblasts on novel 3D in vitro collagen-free scaffolds

Ferretti,

Moccia,

Centelleghe

et al. 2024

PLoS ONE

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Dolphins, as apex predators, can be considered relevant sentinels of the health of marine ecosystems. The creation of 3D cell models to assess in vitro cell-to-cell and cell-to-matrix interactions in environmental-mimicking conditions, is of considerable interest. However, to date the establishment of cetacean 3D culture systems has not yet been accomplished. Thus, in this study, different 3D systems of bottlenose dolphin (Tursiops truncatus) skin fibroblasts have been analyzed. Particularly, novel scaffolds based on hyaluronic acid and ionic-complementary self-assembling peptides such as RGD-EAbuK and EAbuK-IKVAV have been compared to Matrigel. Histological and fluorescent staining, electron microscopy (TEM) analyses and viability assays have been performed and RT-PCR has been used to detect extracellular matrix (ECM) components produced by cells. Results showed that Matrigel induced cells to form aggregates with lower viability and no ECM production compared to the novel scaffolds. Moreover, scaffolds allowed dispersed cells to produce a collagenous ECM containing collagen1a1, laminin B1 and elastin. The HA-EAbuK-IKVAV scaffold resulted in the most suitable 3D model in terms of cell quantity and viability. The development of this innovative approach is the first step towards the possibility to create 3D in vitro models for this protected species.

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Section: Discussionsupporting

confidence: 89%

Bottlenose dolphin (Tursiops truncatus) immortalized fibroblasts on novel 3D in vitro collagen-free scaffolds

Ferretti,

Moccia,

Centelleghe

et al. 2024

PLoS ONE

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“…Although Rab3A plays a major role in regulating exocytosis in a number of neuronal and endocrine cells (Baldini et al, 1995;Leenders et al, 2001;Pavlos et al, 2010), as well as in sperm (Bustos et al, 2012), it also has a role in cancer cells: Rab3A is upregulated in insulinoma but not in normal islets of pancreatic tissue (Lankat-Buttgereit et al, 1994). Rab3A protein is overexpressed in aggressive breast stem-like tumor cells and in epithelial breast cancer cells in response to Heregulin (Vadlamudi et al, 2000) as well as in myofibroblasts (Ina et al, 2005). Interestingly, we found a modest increase in Rab3A protein Statistical analysis was performed using the Student's t-test; *P,0.05.…”

Section: Rab3a Interacts With the Spire-1 C-terminal Regionmentioning

confidence: 99%

Spire-1 a novel contributor of invadosome and associated invasive properties

Lagal

Abrivard

Gonzalez

et al. 2013

Journal of Cell Science

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Cancer cells have an increased ability to squeeze through extracellular matrix gaps that they create by promoting proteolysis of its components. Major sites of degradation are specialized microdomains in the plasma membrane collectively named invadosomes where the Arp2/3 complex and formin proteins cooperate to spatiotemporally control actin nucleation and the folding of a dynamic Factin core. At invadosomes, proper coupling of exo-endocytosis allows polarized delivery of proteases that facilitate degradation of ECM and disruption of the cellular barrier. We investigated the contribution of the actin nucleator Spire-1 to invadosome structure and function, using Src-activated cells and cancer cells. We found that Spire-1 is specifically recruited at invadosomes and is part of a multi-molecular complex containing Src kinase, the formin mDia1 and actin. Spire-1 interacts with the Rab3A GTPase, a key player in the regulation of exocytosis that is present at invadosomes. Finally, over-and under-expression of Spire-1 resulted in cells with an increased or decreased potential for matrix degradation, respectively, therefore suggesting a functional interplay of Spire-1 with both actin nucleation and vesicular trafficking that might impact on cell invasive and metastatic behavior.

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“…Moreover, the ECM of granulation tissue is known to be secreted and modied by broblast cells, which may explain the preference of undifferentiated MC3T3-E1 cells for this material. 57…”

Section: Biological Relevance Of the Resultsmentioning

confidence: 99%