Intracellular fluorescent labelling of cells for analysis of lymphocyte migration

Brenan, Mary; Parish, C R

doi:10.1016/0022-1759(84)90364-8

Cited by 72 publications

(47 citation statements)

References 14 publications

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“…An alternative method well suited to imaging the immune system in situ is the adoptive transfer of vital dye-labeled cells into a normal recipient. Lymphocyte staining with bright fluorescent dyes such as CFSE (green) or SNARF-1 (red) was shown to have minimal deleterious effects on the function of these cells after adoptive transfer in a normal host and to permit tracking of these cells over an extended time, provided they did not divide extensively [76]. However, because the intensity of dye labeling decreases with each cell division, this method is not suitable for following antigen-stimulated cells beyond a very early time after Ag-activation, because they become too dim for microscopic methods.…”

Section: Multiphoton Imagingmentioning

confidence: 99%

Seeing is believing: A focus on the contribution of microscopic imaging to our understanding of immune system function

Bajénoff

Germain

2007

Eur. J. Immunol.

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Section: Multiphoton Imagingmentioning

confidence: 99%

Seeing is believing: A focus on the contribution of microscopic imaging to our understanding of immune system function

Bajénoff

Germain

2007

Eur. J. Immunol.

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“…USA 87 (1990) HL-60 cells 48 hr posttransfection. HL-60 cells were labeled with carboxyfluorescein diacetate (19) (17,22). To extend the range of direct expression cloning methods, and to obviate the need for mAb generation, we examined the feasibility of using a functional assay to clone cell surface molecules involved in cell-cell adhesion.…”

mentioning

confidence: 99%

Endothelial leukocyte adhesion molecule 1: direct expression cloning and functional interactions.

Hession

Osborn

Goff

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

139

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A cDNA for endothelial leukocyte adhesion molecule 1 (ELAM-1) was isolated by transient expression in COS-7 cells of a subtracted cDNA library from cytokinetreated human umbilical vein endothelial cells (HUVECs), with selection of ELAM-1-expressing clones by adhesion of transfected cells to the human promyelocytic cell line HL-60. This cloning method requires neither antibody nor purified ligand. ELAM-1-expressing COS cells bind the promyelocytic cell line HL-60 by a Ca2+-dependent but temperature-independent mechanism. Although ELAM-1 is homologous to mammalian lectins, its interaction with HL-60 cells is not inhibited by simple carbohydrate structures. ELAM-1-expressing COS cells also bind human neutrophils and the human colon carcinoma cell line HT-29, but not the B-cell line Ramos. However, Ramos cells adhere to cytokine-treated HUVECs but not control HUVECs, confirming the existence of other inducible adhesion molecules. In addition, the binding of HL-60 cells or neutrophils to ELAM-1-expressing COS cells is not inhibited by a monoclonal antibody (60.3) directed to an inhibitory epitope on CD18, indicating that the ELAM-1 ligand, although uncharacterized, is not a member of the CD11/CD18 family.

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“…injection. The uorescence was stable for several weeks if protected from light (Brenan and Parish, 1984).…”

Section: Labeling Of Tall-104 Cellsmentioning

confidence: 99%

Antitumor activity of a human cytotoxic T-cell line (TALL-104) in brain tumor xenografts

Geoerger¹,

Tang²,

Cesano³

et al. 2000

Neuro-Oncol

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Intracellular fluorescent labelling of cells for analysis of lymphocyte migration

Cited by 72 publications

References 14 publications

Seeing is believing: A focus on the contribution of microscopic imaging to our understanding of immune system function

Seeing is believing: A focus on the contribution of microscopic imaging to our understanding of immune system function

Endothelial leukocyte adhesion molecule 1: direct expression cloning and functional interactions.

Antitumor activity of a human cytotoxic T-cell line (TALL-104) in brain tumor xenografts

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