Intracellular distribution of Tankyrases as detected by multicolor immunofluorescence techniques

Bottone, Maria Grazia; Santin, Giada; Soldani, Cristiana; Veneroni, Paola; Scovassi, A. Ivana; Alpini, Claudia

doi:10.4081/ejh.2012.e4

Cited by 11 publications

(6 citation statements)

References 19 publications

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“…In cells treated with an apoptogenic stimulus, the GA undergoes fragmentation and redistributes in the cytoplasm, sometimes forming dense aggregates [7,11,12]. …”

Section: Golgi Apparatusmentioning

confidence: 99%

“…We have observed [ 7 , 11 ] that in control HeLa cells the GA exhibits its typical “ribbon like” shape, with flattened cisternae mainly located in the perinuclear area, whereas the vesicles immunopositive for the Golgi markers are progressively less numerous, toward the cell periphery. In cells treated with an apoptogenic stimulus, the GA undergoes fragmentation and redistributes in the cytoplasm, sometimes forming dense aggregates [ 7 , 11 , 12 ].…”

Section: Golgi Apparatusmentioning

confidence: 99%

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Morphological Features of Organelles during Apoptosis: An Overview

Bottone

Santin

Aredia

et al. 2013

Cells

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An apoptotic program leading to controlled cell dismantling implies perturbations of nuclear dynamics, as well as changes affecting the organelle structure and distribution. In human cancer cells driven to apoptosis by different stimuli, we have recently investigated the morphological properties of several organelles, including mitochondria, lysosomes, endoplasmic reticulum and Golgi apparatus. In this review, we will discuss the body of evidence in the literature suggesting that organelles are generally relocated and/or degraded during apoptosis, irrespectively of the apoptogenic stimulus and cell type.

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“…In cells treated with an apoptogenic stimulus, the GA undergoes fragmentation and redistributes in the cytoplasm, sometimes forming dense aggregates [7,11,12]. …”

Section: Golgi Apparatusmentioning

confidence: 99%

Section: Golgi Apparatusmentioning

confidence: 99%

Morphological Features of Organelles during Apoptosis: An Overview

Bottone

Santin

Aredia

et al. 2013

Cells

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“…TNKS-1 maps to endoplasmic reticulum, Golgi, secretion vesicles, epithelial lateral membrane or lysosomes ( Bottone et al, 2012 ; Chi & Lodish, 2000 ; Hsiao & Simth, 2008 ; Vyas et al, 2013 ; Yeh et al, 2006 ). TNKS-1 can also be recruited to the nucleus by TRF1 (telomere repeat binding factor (1) and accompany NuMa (Nuclear/Mitotic apparatus protein) in spindle poles ( Hsiao & Simth, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

VERO cells harbor a poly-ADP-ribose belt partnering their epithelial adhesion belt

Lafon-Hughes

Larrea

Kun

et al. 2014

PeerJ

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Poly-ADP-ribose (PAR) is a polymer of up to 400 ADP-ribose units synthesized by poly-ADP-ribose-polymerases (PARPs) and degraded by poly-ADP-ribose-glycohydrolase (PARG). Nuclear PAR modulates chromatin compaction, affecting nuclear functions (gene expression, DNA repair). Diverse defined PARP cytoplasmic allocation patterns contrast with the yet still imprecise PAR distribution and still unclear functions. Based on previous evidence from other models, we hypothesized that PAR could be present in epithelial cells where cadherin-based adherens junctions are linked with the actin cytoskeleton (constituting the adhesion belt). In the present work, we have examined through immunofluorescence and confocal microscopy, the subcellular localization of PAR in an epithelial monkey kidney cell line (VERO). PAR was distinguished colocalizing with actin and vinculin in the epithelial belt, a location that has not been previously reported. Actin filaments disruption with cytochalasin D was paralleled by PAR belt disruption. Conversely, PARP inhibitors 3-aminobenzamide, PJ34 or XAV 939, affected PAR belt synthesis, actin distribution, cell shape and adhesion. Extracellular calcium chelation displayed similar effects. Our results demonstrate the existence of PAR in a novel subcellular localization. An initial interpretation of all the available evidence points towards TNKS-1 as the most probable PAR belt architect, although TNKS-2 involvement cannot be discarded. Forthcoming research will test this hypothesis as well as explore the existence of the PAR belt in other epithelial cells and deepen into its functional implications.

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“…118–124 Cytogenetic techniques have been used to identify repetitive DNA sequences in the genome of humans and other vertebrates. 121,125,126 …”

Section: A Survey Of the Recent Contents Of A Long-time Established Hmentioning

confidence: 99%

On the future contents of a small journal of histochemistry

Pellicciari

2012

Eur J Histochem

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In the last three years, more than 70,000 scientific articles have been published in peer reviewed journals on the application of histochemistry in the biomedical field: most of them did not appear in strictly histochemical journals, but in others dealing with cell and molecular biology, medicine or biotechnology. This proves that histochemistry is still an active and innovative discipline with relevance in basic and applied biological research, but also demonstrates that especially the small histochemical journals should likely reconsider their scopes and strategies to preserve their authorship. A review of the last three years volumes of the European Journal of Histochemistry, taken as an example of a long-time established small journal, confirmed that the published articles were widely heterogeneous in their topics and experimental models, as in this journal's tradition. This strongly suggests that a journal of histochemistry should keep its role as a forum open to an audience as broad as possible, publishing papers on cell and tissue biology in a wide variety of models. This will improve knowledge of the basic mechanisms of development and differentiation, while helping to increase the number of potential authors since scientists who generally do not use histochemistry in their research will find hints for the applications of histochemical techniques to novel still unexplored subjects.

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Intracellular distribution of Tankyrases as detected by multicolor immunofluorescence techniques

Cited by 11 publications

References 19 publications

Morphological Features of Organelles during Apoptosis: An Overview

Morphological Features of Organelles during Apoptosis: An Overview

VERO cells harbor a poly-ADP-ribose belt partnering their epithelial adhesion belt

On the future contents of a small journal of histochemistry

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