Intracellular Distribution of Rat-Liver Polyribosomes Synthesizing Coeruloplasmin

Gaitskhoki, V. S.; L'vov, V. M.; Monakhov, N. K.; Puchkova, L. V.; Neifakh, S. A.; Frolova, L. Ju.; Skobeleva, N. A.; Zagórski, Włodzimierz

doi:10.1111/j.1432-1033.1981.tb06194.x

Cited by 9 publications

(2 citation statements)

References 18 publications

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“…Specific polyclonal rabbit antibodies were raised against electrophoretically pure rat Cp preparation (A 610 /A 280 = 0.047) by the method developed by us. 49 Rabbit antibodies to SOD1 and Cox4i1 were obtained from ''Abcam'' (UK). Rabbit antibodies to MT and goat antibodies to COMMD1 were purchased from ''Santa Cruz Biotechnology'' (USA).…”

Section: Biochemical Assaysmentioning

confidence: 99%

The effects of silver ions on copper metabolism in rats

et al. 2014

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The influence of short and prolonged diet containing silver ions (Ag-diet) on copper metabolism was studied. Two groups of animals were used: one group of adult rats received a Ag-diet for one month (Ag-A1) and another group received a Ag-diet for 6 months from birth (Ag-N6). In Ag-A1 rats, the Ag-diet caused a dramatic decrease of copper status indexes that was manifested as ceruloplasmin-associated copper deficiency. In Ag-N6 rats, copper status indexes decreased only 2-fold as compared to control rats. In rats of both groups, silver entered the bloodstream and accumulated in the liver. Silver was incorporated into ceruloplasmin (Cp), but not SOD1. In the liver, a prolonged Ag-diet caused a decrease of the expression level of genes, associated with copper metabolism. Comparative spectrophotometric analysis of partially purified Cp fractions has shown that Cp from Ag-N6 rats was closer to holo-Cp by specific enzymatic activities and tertiary structure than Cp from Ag-A1 rats. However, Cp of Ag-N6 differs from control holo-Cp and Cp of Ag-A1 in its affinity to DEAE-Sepharose and in its binding properties to lectins. In the bloodstream of Ag-N6, two Cp forms are present as shown in pulse-experiments on rats with the liver isolated from circulation. One of the Cp isoforms is of hepatic origin, and the other is of extrahepatic origin; the latter is characterized by a faster rate of secretion than hepatic Cp. These data allowed us to suggest that the disturbance of holo-Cp formation in the liver was compensated by induction of extrahepatic Cp synthesis. The possible biological importance of these effects is discussed.

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Section: Biochemical Assaysmentioning

confidence: 99%

The effects of silver ions on copper metabolism in rats

et al. 2014

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“…Cp is synthesized on the polyribosomes associated with the membranes of the rough endoplasmic reticulum; it is directed there by the 19-residue signal peptide, which is removed co-translationally [ 169 ]. The mature translation products of Cp-mRNA and GPI-Cp-mRNA have an approximate molecular mass of 132 kDa.…”

Section: Ceruloplasmin Is a Moonlighting Multicopper Blue Oxidasementioning

confidence: 99%

Abnormalities in Copper Status Associated with an Elevated Risk of Parkinson’s Phenotype Development

Karpenko,

Muruzheva,

Ilyechova

et al. 2023

Antioxidants

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In the last 15 years, among the many reasons given for the development of idiopathic forms of Parkinson’s disease (PD), copper imbalance has been identified as a factor, and PD is often referred to as a copper-mediated disorder. More than 640 papers have been devoted to the relationship between PD and copper status in the blood, which include the following markers: total copper concentration, enzymatic ceruloplasmin (Cp) concentration, Cp protein level, and non-ceruloplasmin copper level. Most studies measure only one of these markers. Therefore, the existence of a correlation between copper status and the development of PD is still debated. Based on data from the published literature, meta-analysis, and our own research, it is clear that there is a connection between the development of PD symptoms and the number of copper atoms, which are weakly associated with the ceruloplasmin molecule. In this work, the link between the risk of developing PD and various inborn errors related to copper metabolism, leading to decreased levels of oxidase ceruloplasmin in the circulation and cerebrospinal fluid, is discussed.

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Identification of ceruloplasmin messenger RNA sequences in heterogeneous nuclear RNA from rat liver

et al. 1981

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The distribution of the sequences of ceruloplasmin mRNA in different fractions of heterogeneous nuclear RNA from rat liver was studied using cDNA transcripts of highly purified mRNA as hybridization probe. The content of ceruloplasmin mRNA sequences in poly(A)-containing and poly(A)-free subfractions of heterogeneous nuclear RNA is respectively 1 and 27 molecules per a hepatocyte. Heterogeneous nuclear RNA carrying the sequences of ceruloplasmin mRNA sedimented in sucrose gradients containing formamide, as a broad zone around the 56S peak. Denaturing electrophoresis followed by the transfer of RNA onto diabenzyloxymethyl paper and hybridization with [32P]-cDNA revealed multiple high molecular weight fractions of ceruloplasmin pre = mRNA (9.0, 6.6, 2.2 and 1.6 megadaltons) in the non-adenylated fraction of nuclear RNA and a single 1.1-1.2 megadalton zone in poly(A)-containing nuclear RNA, the latter being equal in size to the mature ceruloplasmin mRNA from liver polysomes.

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Intracellular Distribution of Rat-Liver Polyribosomes Synthesizing Coeruloplasmin

Cited by 9 publications

References 18 publications

The effects of silver ions on copper metabolism in rats

The effects of silver ions on copper metabolism in rats

Abnormalities in Copper Status Associated with an Elevated Risk of Parkinson’s Phenotype Development

Identification of ceruloplasmin messenger RNA sequences in heterogeneous nuclear RNA from rat liver

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