Intracellular distribution, geno- and cytotoxic effects of nanosized titanium dioxide particles in the anatase crystal phase on human nasal mucosa cells

Hackenberg, Stephan; Friehs, Gudrun; Froelich, Katrin; Ginzkey, Christian; Koehler, Christian; Scherzed, Agmal; Burghartz, Marc; Hagen, Rudolf; Kleinsasser, Norbert

doi:10.1016/j.toxlet.2010.02.022

Cited by 84 publications

(52 citation statements)

References 40 publications

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“…4a), it is not possible to unambiguously identify whether the CeO 2 NPs locate inside the cell nucleus or not. According to many previous studies, 40 nm seems to be the critical size for particles to cross the nucleus pores (Geiser et al 2005;Hackenberg et al 2010;Pante and Kann 2002). Therefore, the CeO 2 NPs in the aggregate state in our study can hardly penetrate through the nucleus pores.…”

Section: Intracellular Localizationmentioning

confidence: 49%

“…However, the safety issues of the nanomaterials have given rise to increasing concerns for regulators, general public and also producers who inevitably contact with these materials (Hackenberg et al 2010;Xia et al 2008). Hence, clear understanding of the relationship between the nanomaterials characteristics and their interactions with biological system is strongly demanded, so that the safety issue can be addressed to a maximum extent.…”

Section: Introductionmentioning

confidence: 99%

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Uptake of cerium oxide nanoparticles and its influence on functions of mouse leukemic monocyte macrophages

et al. 2015

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Exposure of the CeO 2 nanoparticles (NPs) causes a public concern on their potential health risk due to their wide applications in the fields of fuel additive, commodities, pharmaceutical, and other industries. In this study, the interactions between two commercial CeO 2 NPs (D-CeO 2 from Degussa and PC-CeO 2 from PlasmaChem) and mouse leukemic monocyte macrophage Raw264.7 cells were investigated to provide a fast and in-depth understanding of the biological influences of the NPs. Both types of the CeO 2 NPs had a negative surface charge around -12 mV and showed a tendency to form aggregates with sizes of 191 ± 5.9 and 60.9 ± 2.8 nm in cell culture environment, respectively. The cellular uptake of the CeO 2 NPs increased along with the increase of feeding dosage and prolongation of the culture time. The PC-CeO 2 NPs had a faster uptake rate and reached higher cellular loading amount at the highest feeding concentration (200 lg/mL). In general, both types of the CeO 2 NPs had rather small cytotoxicity even with a dosage as high as 200 lg/mL. The D-CeO 2 NPs showed a relative stronger cytotoxicity especially at higher concentrations and longer incubation time. The NPs were dispersed in vacuoles (most likely endosomes and lysosomes) and cytoplasm. Although both types of the CeO 2 NPs could suppress the production of reactive oxygen species, they impaired the mitochondria membrane potential to some extent. The cytoskeleton organization was altered and consequently the cell adhesion ability decreased after uptake of both types of the CeO 2 NPs.

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Section: Intracellular Localizationmentioning

confidence: 49%

Section: Introductionmentioning

confidence: 99%

Uptake of cerium oxide nanoparticles and its influence on functions of mouse leukemic monocyte macrophages

et al. 2015

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“…In HNSCC, 3.5% of cells contained NP aggregates in the nucleus compared to 0.5% in pOMCs. According to a recent report on the toxicological properties of TiO 2 NPs, measurable DNA damage can be shown even if a minor fraction of the cells is exposed to a genotoxic substance (29). However, the mechanisms of nuclear particle uptake remain to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Zinc oxide nanoparticles induce photocatalytic cell death in human head and neck squamous cell carcinoma cell lines in vitro

Kleinsasser¹

2010

Int J Oncol

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Abstract. The aim of this study was to determine the photocatalytic effects of zinc oxide (ZnO) NPs in combination with UVA-1 in human head and neck squamous cell carcinoma (HNSCC) cell lines in vitro. NP characteristics and intracellular distribution were described by transmission electron microscopy (TEM). After pre-incubation with ZnO NPs in concentrations of 0.002-20 μg/ml, the HNSCC cell lines HLaC 78 and UD-SCC 7A as well as primary oral mucosa cells (pOMCs) were treated with UVA-1. Cell survival and vitality was observed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide-(MTT)-assay and fluorescein diacetate test. Apoptosis was assessed by annexin-V propidium iodide flow cytometry. Intranuclear distribution of the rod-shaped particles was observed in 3.5% of HNSCC and in 0.5% of pOMCs. UVA-1 irradiation of 15 min in combination with 0.2 and 2 μg/ml of ZnO NP dispersion was shown to reduce the vitality of cancer cell lines significantly in comparison to cells without NP exposure or UVA-1 treatment only. For HLaC 78, a significant reduction in viable cells was already seen at 10 min of UVA-1 treatment and a ZnO NP concentration of 2 μg/ml. Flow cytometry indicated that cell death occurred primarily through necrosis. In pOMCs, vitality was not influenced either by UVA-1 treatment or ZnO NP exposure up to 2 μg/ml or a combination of both. ZnO NPs showed cytotoxicity at 20 μg/ml without UVA-1. Due to their photocatalytic properties, ZnO NPs may induce cell death in human HNSCC cell lines in vitro. Further studies will evaluate a possible benefit in adjuvant cancer therapy.

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“…Some people argue that a risk exists, zinc oxide could have potentially genotoxic effects on human epidermal cells [86] and titanium dioxide could be cytotoxic and genotoxic on cell cultures (hamster ovary cells) [87]. For others, these ingredients are totally safe [88,89]. Concerning efficacy, titanium dioxide proves to be the most efficient, as it gives an SPF value of 10 (Eusolex TS ® ) as opposed to 5 for zinc oxide (Z-Cote Max ® ) for the same incorporation percentage of 10%.…”

Section: Inorganic Filters or Screensmentioning

confidence: 99%

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Couteau¹,

Coiffard²

2013

Highlights in Skin Cancer

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Intracellular distribution, geno- and cytotoxic effects of nanosized titanium dioxide particles in the anatase crystal phase on human nasal mucosa cells

Cited by 84 publications

References 40 publications

Uptake of cerium oxide nanoparticles and its influence on functions of mouse leukemic monocyte macrophages

Uptake of cerium oxide nanoparticles and its influence on functions of mouse leukemic monocyte macrophages

Zinc oxide nanoparticles induce photocatalytic cell death in human head and neck squamous cell carcinoma cell lines in vitro

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