Intracellular development of a large DNA bacteriophage lytic for Caulobacter crescentus

Poindexter, Jeanne S.; Hornack, P. R.; Armstrong, P.

doi:10.1007/bf00406337

Cited by 15 publications

(11 citation statements)

References 13 publications

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“…ϕCb13 and ϕCbK preferentially infect swarmer cells (6,30), where a correlation exists between flagellar rotation and the ability of ϕCbK to successfully infect cells and generate progeny (6). It has been established that WT C. crescentus cells have a swimming reversal frequency of 50% (31).…”

Section: Resultsmentioning

confidence: 99%

Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus

Guerrero-Ferreira

Viollier

Ely

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

116

137

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2D and 3D cryo-electron microscopy, together with adsorption kinetics assays of ϕCb13 and ϕCbK phage-infected Caulobacter crescentus , provides insight into the mechanisms of infection. ϕCb13 and ϕCbK actively interact with the flagellum and subsequently attach to receptors on the cell pole. We present evidence that the first interaction of the phage with the bacterial flagellum takes place through a filament on the phage head. This contact with the flagellum facilitates concentration of phage particles around the receptor (i.e., the pilus portals) on the bacterial cell surface, thereby increasing the likelihood of infection. Phage head filaments have not been well characterized and their function is described here. Phage head filaments may systematically underlie the initial interactions of phages with their hosts in other systems and possibly represent a widespread mechanism of efficient phage propagation.

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Section: Resultsmentioning

confidence: 99%

Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus

Guerrero-Ferreira

Viollier

Ely

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

116

137

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“…The observation that some stage-specific proteins are retained during differentiation is in contrast to the regulation of flagellar organelle expression in Caulobacter. It has long been known that the flagellar filament, hook, and rod structures are simply expelled from the cell during the differentiation of the stalked cell (21,24,30). The flagellum is not an obligatory structural component of the cell, as W88 may be, and can be deleted by mutation without consequence to the developmental cycle (13,20,30).…”

Section: Discussionmentioning

confidence: 99%

Generation of asymmetry during development. Segregation of type-specific proteins in Caulobacter.

Agabian¹,

Evinger²,

Parker³

1979

The Journal of Cell Biology

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An essential event in developmental processes is the introduction of asymmetry into an otherwise undifferentiated cell population. Cell division in Caulobacter is asymmetric; the progeny cells are structurally different and follow different sequences of development, thus providing a useful model system for the study of differentiation. Because the progeny cells are different from one another, there must be a segregation of morphogenetic and informational components at some time in the cell cycle. We have examined the pattern of specific protein segregation between Caulobacter stalked and swarmer daughter cells, with the rationale that such a progeny analysis would identify both structurally and developmentally important proteins. To complement the study, we have also examined the pattern of protein synthesis during synchronous growth and in various cellular fractions.We show here, for the first time, that the association of proteins with a specific cell type may result not only from their periodicity of synthesis, but also from their pattern of distribution at the time of cell division. Several membraneassociated and soluble proteins are segregated asymmetrically between progeny stalked and swarmer cells. The data further show that a subclass of soluble proteins becomes associated with the membrane of the progeny stalked cells. Therefore, although the principal differentiated cell types possess different synthetic capabilities and characteristic proteins, the asymmetry between progeny stalked and swarmer cells is generated primarily by the preferential association of specific soluble proteins with the membrane of only one daughter cell. The majority of the proteins which exhibit this segregation behavior are synthesized during the entire cell cycle and exhibit relatively long, functional messenger RNA half-lives.KEY WORDS differentiation 9 polarity unequal protein distribution progeny analysis From the beginnings of experimental embryology, the study of development has been concerned with the mechanisms by which cells generate and maintain structural and functional differences. The molecular basis for the generation of these differences is not known, but implicit in this view of differentiation is a requirement that at some period in the development of an organism a special subclass of cellular components is asym-J. CELL BIOLOGY 9 The Rockefeller University Press 9

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“…Some workers have found additional Ca (0.5 mM) supplemenitation useful (109). For the past several yeaws, the PYE medium used in my laboratory has been prepared in distilled water supplemented with vitamin-free Hutner base (32) rather than MgSO4 alone (185,224). This practice, also used in J. M. Schmidt's laboratory (26,29,54,119) and occasionally by others (180), has reduced variation in mineral content of the medium and improved reproducibility of both rate and yield of growth with all strains used.…”

Section: Cultivation and Nutritionmentioning

confidence: 99%

The caulobacters: ubiquitous unusual bacteria.

Poindexter¹

1981

Microbiological Reviews

198

115

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Intracellular development of a large DNA bacteriophage lytic for Caulobacter crescentus

Cited by 15 publications

References 13 publications

Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus

Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus

Generation of asymmetry during development. Segregation of type-specific proteins in Caulobacter.

The caulobacters: ubiquitous unusual bacteria.

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