Intracellular delivery of quantum dots mediated by a histidine- and arginine-rich HR9 cell-penetrating peptide through the direct membrane translocation mechanism

Liu, Betty Revon; Huang, Yue-Wern; Winiarz, Jeffrey G.; Chiang, Hsin‐Ju; Lee, Han-Jung

doi:10.1016/j.biomaterials.2011.01.041

Cited by 145 publications

(181 citation statements)

References 71 publications

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“…However, for the latter, the noncovalent strategy for cargo and carrier has been used less often than the covalent one. CPPs could also be used to enhance other drug delivery systems such as polymer-based systems (e.g., micelles, dendrimers), liposomes , and inorganic carriers such as gold-, silver-, and ironbased nanoparticles (superparamagnetic iron oxide nanoparticles) (Wagner et al, 2009;Shirazi et al, 2014;Zuo et al, 2014), and quantum dots (Delehanty et al, 2006;Liu et al, 2011Liu et al, , 2013aMartin et al, 2013). Most nanoparticles provide binding sites for different cargos and targeting peptides which can be used for diagnostics and therapy.…”

Section: Formation Of Carrier-cargo Complexesmentioning

confidence: 99%

Viral and Other Cell-Penetrating Peptides as Vectors of Therapeutic Agents in Medicine

Durzyńska

Przysiecka

Nawrot

et al. 2015

J Pharmacol Exp Ther

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Efficient delivery of heterologous molecules for treatment of cells is a great challenge in modern medicine and pharmacology. Cellpenetrating peptides (CPPs) may improve efficient delivery of a wide range of macromolecular cargos, including plasmid DNA, small interfering RNA, drugs, nanoparticulate pharmaceutical carriers, and anticancer drugs. In this paper, we present the history of CPPs' discovery with special attention drawn to sequences of viral origin. We also describe different CPP families with regard to their physicochemical properties and numerous mechanisms of CPP cell uptake by direct penetration and endocytotic pathways. A detailed description is focused on formation of carrier-cargo complexes, which are needed for practical use of CPPs in medicine and biotechnology. Examples of successful application of CPPs in treatment of human diseases are also presented, including decreased tumor growth and induction of cancer cell death. Finally, we review modern design approaches to novel CPPs and prediction of their activity. To sum up, the current review presents a thorough and up-to-date knowledge of CPPs and may be a valuable source of information for researchers in pharmacology designing new therapeutic agents.

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Section: Formation Of Carrier-cargo Complexesmentioning

confidence: 99%

Viral and Other Cell-Penetrating Peptides as Vectors of Therapeutic Agents in Medicine

Durzyńska

Przysiecka

Nawrot

et al. 2015

J Pharmacol Exp Ther

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“…This buffering capacity results in a "proton sponge effect" and destabilizes the membranes of the endosome/lysosome in the acidic environment and allows the NPs or complexes to escape from the endosome/lysosome rapidly. [32][33][34] In this study, we incorporated histidine residues into 6-polyarginine to design a novel H6R6 peptide to increase intracellular uptake and help NP escape from the endosome/lysosome and to enable the abundant release of siRNA into the cytoplasm to increase its bioavailability.…”

mentioning

confidence: 99%

siRNA-loaded poly(histidine-arginine)<sub>6</sub>-modified chitosan nanoparticle with enhanced cell-penetrating and endosomal escape capacities for suppressing breast tumor metastasis

Park¹,

Huang²,

Kang³

et al. 2017

IJN

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An ideal carrier that delivers small interfering RNA (siRNA) should be designed based on two criteria: cellular-mediated internalization and endosomal escape. Poly(histidine-arginine) 6 (H6R6) peptide was introduced into chitosan (CS) to create a new CS derivative for siRNA delivery, 6-polyarginine (R6) as cell-penetrating peptides facilitated nanoparticle cellular internalization has been proved in our previous research, and 6-polyhistidine (H6) mediated the nanoparticle endosome escape resulted in the siRNA rapid releasing into tumor cytoplasm. H6R6-modified CS nanoparticles showed higher transfection efficiency and better endosomal escape capacity compared to ungroomed CS nanoparticle in vitro. Noticeably, H6R6-modified CS nanoparticles effectively inhibited tumor cell growth and metastases in vivo and significantly improved survival ratio. Therefore, we concluded that H6R6-modified CS copolymer can act as an ideal carrier for siRNA delivery and as a promising candidate in breast cancer therapy.

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“…To examine the mechanism of membrane translocation of 1_GFP, the uptake efficiency of 1_GFP was evaluated in the presence of endocytic inhibitors ( Figure S7). 26,27 Low temperature and cytochalasin D are commonly used inhibitors of endocytosis. 26,27 Uptake of 1_GFP was only partially inhibited (52%) by incubation at 4°C, although cytochalasin D showed no effect.…”

mentioning

confidence: 99%

“…26,27 Low temperature and cytochalasin D are commonly used inhibitors of endocytosis. 26,27 Uptake of 1_GFP was only partially inhibited (52%) by incubation at 4°C, although cytochalasin D showed no effect. Rottlerin, which is an inhibitor of macropinocytosis, also showed partial inhibition (49%).…”

mentioning

confidence: 99%

“…These results indicate that the uptake of 1_GFP involves both macropinocytosis and direct endocytosis-independent membrane translocation. Since the direct membrane translocation does not cause endosomal entrapment, 27 some 1_GFPs are expected to directly translocate to the cytoplasm then to subsequently release sfGFPs. To confirm whether sfGFP is cleaved from 1_GFP in the cells, GPC analyses of the supernatant and the cell lysates were carried out to detect sfGFP fragments after incubation of the cells with 1_GFP (Figure 3c).…”

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confidence: 99%

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Intracellular Protein Delivery System with Protein Needle–GFP Construct

et al. 2014

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A protein delivery system with a protein needle has been constructed. Green fluorescent protein (GFP) was genetically fused to a cell-penetrating protein needle. The construct is stable in buffer and cell culture medium. Internalization of the construct in HeLa cells and subsequent release of GFP from the needle was observed. The study provides a useful tool for intracellular protein delivery in an efficient manner and has potential for use in vitro and in vivo applications.

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Intracellular delivery of quantum dots mediated by a histidine- and arginine-rich HR9 cell-penetrating peptide through the direct membrane translocation mechanism

Cited by 145 publications

References 71 publications

Viral and Other Cell-Penetrating Peptides as Vectors of Therapeutic Agents in Medicine

Viral and Other Cell-Penetrating Peptides as Vectors of Therapeutic Agents in Medicine

siRNA-loaded poly(histidine-arginine)<sub>6</sub>-modified chitosan nanoparticle with enhanced cell-penetrating and endosomal escape capacities for suppressing breast tumor metastasis

Intracellular Protein Delivery System with Protein Needle–GFP Construct

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