Intracellular delivery of a native functional protein using cell-penetrating peptide functionalized cubic MSNs with ultra-large mesopores

Abstract

Pore-enlarged 3D cubic cMSNs were successfully prepared, and their surfaces were functionalized by a cell-penetrating R8-peptide through the click reaction for cytosolic delivery of a functional protein in its native form.

Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme

“…22 Many studies have investigated the enzymatic activity and stability of MS platforms. [23][24][25][26][27][28] In general, the results have shown that adsorption, covalent bonding and entrapment mechanisms all provide an effective means of stabilizing enzymes on inorganic, organic or polymeric matrices by increasing the rigidity of the matrix and reducing the unfolding and deactivating possibility. [29][30][31][32] Diaz and Mansor examined the MS entrapment of three different enzymes, namely cytochrome C, papain and trypsin 33,34 It was shown that the trypsin retained a signicant activity for more than one week in the immobilized state, but was totally deactivated aer just 24 h in the free (i.e., solution) state.…”

Simple Preparation of Large Pore Mesoporous Silica Nanospheres and Their Protein Sequestration Ability

“…In comparison, Ca(NO 3 ) 2 -treated cMSN showed BET surface area of 225 m 2 g À 1 and pore dimension of 13.89 nm. [20] The corresponding pore volume was 0.59 cm 3 g À 1 . These data clearly indicate that Ca-cMSN was slightly less effectively etched compared to Ca(NO 3 ) 2 -treated cMSN, possibly due to different counter-anions of calcium salts.…”

“…The as-prepared cMSN was prepared by using cetyltrimethylammonium bromide (CTAB) and F127 in basic aqueous solution according to the literature. [20,22] We also tested different solvents and reaction temperatures to compare the etching efficiency. We found that CaCl 2 is also a good etching source for the preparation of large pore cMSN (Ca-cMSN).…”

Design and applications of protein delivery systems in nanomedicine and tissue engineering

“…Another technique to formulate mineral particles is ion etching. More precisely, it can be used as perforation etching to form porous particles [45,46] or as ion etching, as reported by Bae et al, who used calcium ion etching to enlarge pores in cubic MSNs [47].…”

“…It has to be noticed that the detection limits can be different when the protocol is adapted to be used with microplates. It is very used to quantify BSA [8,13,43,47] and insulin [13,59,60,93], but it is also used with other proteins such as chymotrypsin, myoglobin, HRP [13], immunoglobulin G (IgG) and OVA [47].…”