“…Intracellular staining for cytokines and surface expression of CD107a was performed as described elsewhere [56]. In brief, P815 cells, a mouse mastocytoma cell line obtained from the American Type Culture Collection, Manassas, VA, USA, were pre-coated with combinations of antibodies to NKR, 5 mg/mL anti-2B4 (clone C1.7, Beckman Coulter), 5 mg/mL anti-DNAM-1 (DX11, Becton Dickinson), 5 mg/mL anti-NKG2D (1D11, Becton Dickinson), 0.1 or 5 mg/mL anti-CD3 (OKT-3), or 0.1 or 5 mg/mL mouse IgG (MOPC-21, BioLegend, San Diego, CA, USA).…”