Intracellular Components Associated with Chikungunya Virus‐Specific Ribonucleic Acids in Infected BHK21 Cells1

Igarashi, Akira; Sasao, Fuyoko; Fukai, K

doi:10.1111/j.1348-0421.1973.tb00791.x

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…Preliminary studies raised the hypothesis that CHIKV could present tropism for renal tissue, based on detecting the expression of viral proteins and the ability of the virus to replicate in this tissue of animal models. [68][69][70][71] This hypothesis was later strengthened with studies conducted also in human cells, demonstrating that CHIKV has a binding, infectivity, replication and apoptosis induction capacity in renal cells. 72,73 The first case of renal impairment related to CHIK in humans was a boy with nephritic syndrome and no joint symptoms, with a positive PCR for CHIKV.…”

Section: Discussionmentioning

confidence: 99%

The relationship between chikungunya virus and the kidneys: A scoping review

Costa

Gouveia

Silva

et al. 2022

Reviews in Medical Virology

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Several atypical forms of chikungunya fever (CHIK) have been described, including neurological, cardiac and renal involvement. These forms may be related to high morbidity and mortality rates. This scoping review based on the PubMed, Scopus, and WOS databases aims to identify and summarise all the available evidence regarding the clinical and histopathological presentations and risk factors associated with kidney injury related to CHIK, as well as the clinical impact. Thus, a total of 54 papers were selected from 1606 initial references after applying the defined inclusion criteria. Data on the association between kidney injury and CHIK are scarce, with studies only conducted in the acute phase of the disease, lacking further characterisation. Kidney injury incidence in hospitalised patients using the Kidney Disease Improving Global Outcomes criteria varies from 21% to 45%, being higher among patients with atypical and severe manifestations. Although acute kidney injury does not seem to be related to viraemia, it may be related to higher mortality.Few studies have described the renal histopathological changes in the acute phase of CHIK, with prevalent findings of acute interstitial nephritis with mononuclear infiltrate, glomerular congestion and nephrosclerosis. Only one study assessed the kidney function of patients in the subacute and chronic phases of CHIK. Additionally, individuals with comorbidities, including chronic kidney disease, may be among those with a greater risk of presenting worse outcomes when affected by CHIK. The results described herein may contribute to better understand the relationship between the kidneys and chikungunya virus.

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Section: Discussionmentioning

confidence: 99%

The relationship between chikungunya virus and the kidneys: A scoping review

Costa

Gouveia

Silva

et al. 2022

Reviews in Medical Virology

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“…The origins and passage histories of these viruses were already described as well as the method to prepare infected suckling mouse brain (SMB) stock viruses (Igarashi, 1978). The Nakayama strain of Japanese encephalitis ( JE) virus was grown in adult mouse brains, and chikungunya (CHIK) virus, African strain, was plaquepurified and seed virus was prepared in BHK21 cells (Igarashi and Fukai, 1969). Cells: BHK21 cells were grown at 37 C with 10 per cent calf serum in Eagle's (1959) minimal essential medium (MEM).…”

Section: Methodsmentioning

confidence: 99%

Comparative Sensitivity of Focus Counting Method in the Infectivity Titration of Dengue Viruses and in the Neutralization Test

Igarashi

Okuno

Fukai

1979

Jap. J. Trop. Med. Hyg.

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Abstract:Infectivity titrations were performed for each of the 4 prototypes of dengue (DEN) viruses by focus counting on BHK21 cells (FFU), by plaque formation on LLC-MK2 cells (PFU), and by intracerebral inoculation into suckling mice (LD50) The FFU titers were almost similar as LD50 for all the 4 types of DEN and these titers were also similar to PFU except type 2 DEN (DEN-2), which showed lower PFU than FFU or LD50. Neutralization (N) tests were performed for standard anti-DEN sera by 50 per cent focus reduction (FR50) and by 50 per cent plaque reduction (PR50) methods. Both values were almost the same except anti-DEN-2, whcih showed higher PR50 than FR50. The principle of these methods were also applied to Japanese encephalitis and chikungunya viruses. Hemagglutination-inhibition (HI) and FR50-N tests on several human sera indicated that FR50 is more specific and sensitive than HI to detect typespecific antibodies.

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