Intracellular compartmentation and membrane permeability to sugars and acids at different growth stages of peach

Jiang, Feng-chao; Wang, Yuzhu; Sun, Hongjian; Li, Yang; Zhang, Junhuan; Ma, Lvyi

doi:10.1016/j.scienta.2013.06.037

Cited by 10 publications

(9 citation statements)

References 17 publications

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“…These results are at variance with the experimentally (the 'wash out' technique was used) derived results of Jiang et al (2013), who found that a considerable proportion of the sugar content was in the cytoplasm. The results of Jiang et al (2013) imply a very high cytoplasmic sugar concentration (because the cytoplasm only occupies a small proportion of the total cell volume) (Desnoues et al, 2018). Using the non-aqueous fractionation technique, Nadwodnik and Lohaus (2008) found that in mature peach leaves the bulk of the contents of sucrose, glucose, fructose and sorbitol were located in the vacuole.…”

Section: Subcellular Compartmentation Of Non-structural Soluble Carbocontrasting

confidence: 50%

“…Nevertheless, the bulk of the content of each of these sugars would be located in the vacuole because it occupies a very large fraction of cell volume (Desnoues et al, 2018). These results are at variance with the experimentally (the 'wash out' technique was used) derived results of Jiang et al (2013), who found that a considerable proportion of the sugar content was in the cytoplasm. The results of Jiang et al (2013) imply a very high cytoplasmic sugar concentration (because the cytoplasm only occupies a small proportion of the total cell volume) (Desnoues et al, 2018).…”

Section: Subcellular Compartmentation Of Non-structural Soluble Carbomentioning

confidence: 74%

See 1 more Smart Citation

Non-structural Carbohydrate Metabolism in the Flesh of Stone Fruits of the Genus Prunus (Rosaceae) – A Review

et al. 2020

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Section: Subcellular Compartmentation Of Non-structural Soluble Carbocontrasting

confidence: 50%

Section: Subcellular Compartmentation Of Non-structural Soluble Carbomentioning

confidence: 74%

Non-structural Carbohydrate Metabolism in the Flesh of Stone Fruits of the Genus Prunus (Rosaceae) – A Review

et al. 2020

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“…Hexose active transporters were represented by an MM equation with competition between fructose and glucose, as presented by Beauvoit et al (2014). Very little information is available on sorbitol transporters, and none of this information pertains to the tonoplast (Wei et al, 2014); however, a tonoplastic transport mechanism is at least required to transport sorbitol in the vacuoles of peach, so the sorbitol transporter was considered as passive transport (F19) (Jiang et al, 2013).…”

Section: Tonoplastic Transportmentioning

confidence: 99%

A kinetic model of sugar metabolism in peach fruit reveals a functional hypothesis of a markedly low fructose‐to‐glucose ratio phenotype

Desnoues

Génard²,

Quilot-Turion

et al. 2018

The Plant Journal

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The concentrations of sugars in fruit vary with fruit development, environment and genotype. In general, there were weak correlations between the variations in sugar concentrations and the activities of enzymes directly related with the synthesis or degradation of sugars. This finding suggests that the relationships between enzyme activities and metabolites are often non-linear and are difficult to assess. To simulate the concentrations of sucrose, glucose, fructose and sorbitol during the development of peach fruit, a kinetic model of sugar metabolism was developed by taking advantage of recent profiling data. Cell compartmentation (cytosol and vacuole) was described explicitly, and data-driven enzyme activities were used to parameterize equations. The model correctly accounts for both annual and genotypic variations, which were observed in 10 genotypes derived from an interspecific cross. They provided important information on the mechanisms underlying the specification of phenotypic differences. In particular, the model supports the hypothesis that a difference in fructokinase affinity could be responsible for a low fructose-to-glucose ratio phenotype, which was observed in the studied population.

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“…Recently, the distribution of sugars among different compartments (free space, cytoplasm and vacuole) of peach mesocarp at two different growth stages has been determined, providing evidence for a predominant cytoplasmic localization in young fruit and a vacuolar localization in mature fruit. Moreover, the same authors showed that the permeability through plasma membrane and tonoplast decreased with peach fruit maturation (Jiang et al ).…”

Section: Introductionmentioning

confidence: 97%

Sucrose transport and phloem unloading in peach fruit: potential role of two transporters localized in different cell types

Zanon

Falchi

Santi

et al. 2014

Physiologia Plantarum

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Several complex physiological processes, which include long-distance translocation in the phloem and unloading in sink tissues, govern the partitioning of sugars in economically important organs, such as peach fruit. In this study, we took advantage of a symplastic tracer, carboxyfluorescein (CF), providing evidence for an apoplastic sucrose transfer in the early (SI) and middle (SIII) phases of peach fruit development. Moreover, using a combination of in situ hybridization and laser microdissection-assisted expression analysis, three putative sucrose transporters encoding genes (PpSUT1, PpSUT2, PpSUT4) were transcriptionally analyzed to relate their expression with sucrose storage in this organ. Our study revealed that PpSUT2 and PpSUT4 are the genes predominantly expressed in fruit flesh, and the detailed analysis of their expression pattern in the different cell types enabled us to suggest a specialized role in sucrose distribution. Both PpSUTs transporters could be involved in the retrieval of sucrose lost from the symplastic continuum of the phloem and, when expressed in parenchyma cells, they could be active in the import of sucrose into sink tissues, via symport from the apoplast. An alternative hypothesis has been proposed and discussed for PpSUT4 because of its putative tonoplastic localization. Taken together, our results provide new insights into the molecular mechanisms underpinning sucrose unloading and accumulation in peach fruit.

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Intracellular compartmentation and membrane permeability to sugars and acids at different growth stages of peach

Cited by 10 publications

References 17 publications

Non-structural Carbohydrate Metabolism in the Flesh of Stone Fruits of the Genus Prunus (Rosaceae) – A Review

Non-structural Carbohydrate Metabolism in the Flesh of Stone Fruits of the Genus Prunus (Rosaceae) – A Review

A kinetic model of sugar metabolism in peach fruit reveals a functional hypothesis of a markedly low fructose‐to‐glucose ratio phenotype

Sucrose transport and phloem unloading in peach fruit: potential role of two transporters localized in different cell types

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