Intracellular collagen fibrils in the periodontal ligament of man

Eley, B M; Harrison, J. D.

doi:10.1111/j.1600-0765.1975.tb00021.x

Cited by 30 publications

(9 citation statements)

References 4 publications

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“…Although macrophages are the cells characteristically engaged in phagocytosis, fibroblasts have been found to possess a phagocytic potential and to phagocytose collagen fibrils in the turnover of connective tissue (Deporter and Ten Cate, 1973;Eley and Harrison, 1975). The finding that the intracellular particles were often seen to be membrane-bound indicates that they have been phagocytosed and reside in phagosomes.…”

Section: Discussionmentioning

confidence: 99%

Amalgam tattoos: Light and electron microscopy and electron‐probe micro‐analysis

Harrison

Rowley

Peters³

1977

The Journal of Pathology

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A common tattoo occurring in the mouth is caused by the insertion of amalgam filling material into the soft tissues. Fifteen amalgam tattoos were examined. Amalgam or its derivatives were found within macrophages, fibroblasts and multinuclear giant cells. Fine particles were found associated with: the basement-membranes of mucosal epithelium, of striped muscle fibres, and of muscle cells of blood vessels; collagen; elastic tissue; and the connective tissue of nerves. Amalgam consists mainly of mercury, silver and tin. The analytical results suggest that corrosion occurs and that mercury and some tin are lost from the tattoos, leaving silver and tin in macrophages and giant cells, and silver in fibroblasts and in the above-mentioned extracellular sites.

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Section: Discussionmentioning

confidence: 99%

Amalgam tattoos: Light and electron microscopy and electron‐probe micro‐analysis

Harrison

Rowley

Peters³

1977

The Journal of Pathology

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“…In both physiological and pathological conditions collagen fibrils may also be phagocytosed and degraded within cells including fibroblasts (Ten Cate 1972, Eley & Harrison 1975, Melcher & Chan 1981, macrophages (Parakkal 1969, Hentzer & Kobayashi 1979 and osteoclasts (Everts et al 1985). These cells contain cysteine proteinases within their lysosomes and the fact that the digestion of phagocytosed collagen is inhibited by leupeptin, a cysteine and serine proteinase inhibitor (Umezama 1976), and E-64, a more selective cysteine proteinase inhibitor (Barrett et al 1981), suggests that they are of major importance in intracellular collagen degradation (Everts et al 1988).…”

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confidence: 99%

Cathepsin B‐ and L‐like activities at local gingival sites of chronic periodontitis patients

Eley

Cox

1991

J Clinic Periodontology

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The cysteine proteinases cathepsins B and L have the potential to degrade connective tissue in chronic periodontitis and this may progress episodically at individual tooth sites. The activities of cathepsin B- and L-like proteinases in homogenised gingival tissue from control and periodontitis patients were measured biochemically using the selective peptide substrate Z-Phe-Arg-AFC and the selective cathepsin L inhibitor Z-Phe-Phe-CHN2. Each tooth site was divided, where appropriate, into gingival tissue and granulomata. These were assayed separately and the measurements related to the DNA and protein contents of the tissues. Enzyme activity in healthy control tissue was significantly lower than in diseased tissue. Enzyme activity in gingival tissue and total tissue from periodontitis patients decreased with increasing pocket depth, clinical attachment level, gingival index and bleeding index whilst cathepsin B activity in granulomata increased with increasing pocket depth and clinical attachment level but not with increasing gingival index or gingival bleeding index. Mean enzyme activity in gingival tissue was 1.6-2.8 times greater than in granulomata. Mean patient enzyme activity in diseased patients did not correlate positively with their mean pocket depth, clinical attachment level, gingival index or gingival bleeding index. These results are best explained by the probable cellular origins of the enzymes and the likely influence of their serum and tissue inhibitors during the disease process.

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“…Moreover, it is now knov^' that some fibroblasts degrade by endocytotic processes the very collagen they have synthesized. This vi^as shown for fibroblasts in the periodontal ligament in vivo (Listgarten 1973, Beertsen, Everts & van den Hoof 1974, Ten Cate & Deporter 1974, Eley & Harrison 1975, Ten Cate, Deporter & Freeman 1976, Garant 1977, and the coUagenolysis of bovine collagen observed by human gingival fibroblasts in vitro (Rose & Robertson 1977, Yajima & Rose 1977, Rose, Yajima & Mahan 1980 was shown to operate in a similar manner, i. e., through the phagocytotic interiorization of the collagen fibrils and their degradation within lysosomes. In this report, we have illustrated electron micrographic findings relative to the fibrillar synthesis of human gingival fibroblasts and have called attention to new and associated findings which are important for documentation and further consideration.…”

Section: Introductionmentioning

confidence: 63%

Human gingival fibroblast cell lines in vitro

Yajima

Rose

Mahan

1980

J of Periodontal Research

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Eight human gingival fibroblast cell lines were cultivated in glass cover slip chambers and analyzed by electron microscopy for their capacity to synthesize an extracellular fibrillar matrix. Both microfibrils and amorphous components of elastic tissue and several forms of banded and unbanded collagen fibrils were elaborated by the fibroblasts. Banding periods of 64 nm and 100 nm were observed. Collagenases lysed collagen fibrils and in some cases altered them to display prominently banded FLS images. Numerous matrix vesicles were observed among the extracellular collagen fibrils and some were observed intracellularly either isolated in pinocytotic‐like vesicles or as clusters in larger vacuoles. Matrix granules also were observed among the extracellular fibrils. The Golgi sacs of synthesizing fibroblasts contained fine thread‐like parallel filaments with a banding period of 220 nm. The presence of annulate lamellae was related to culture aging and the appearance of large mitochondrial matrix granules was related to the effect of small increments of vitamin C. Two cells were shown to have phagocytosed collagen elaborated by other cells earlier, one contained an interiorized fibril with a 64 nm banding period and the other an interiorized fibril with a 100 nm banding period. A turnover of collagen in vitro was thus established.

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Intracellular collagen fibrils in the periodontal ligament of man

Abstract: Periodontal ligament from 6 teeth extracted from patients was examined electron‐microscopically for the presence of intracellular collagen fibrils. These were occasionally found in fibroblasts in the tissue from 4 teeth, but were not otherwise seen.

Cited by 30 publications

References 4 publications

Amalgam tattoos: Light and electron microscopy and electron‐probe micro‐analysis

Amalgam tattoos: Light and electron microscopy and electron‐probe micro‐analysis

Cathepsin B‐ and L‐like activities at local gingival sites of chronic periodontitis patients

Human gingival fibroblast cell lines in vitro

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