Intracellular CBG-like molecules in the rat pituitary

Abstract

Summary-The localization of transcortin (CBG) in pituitary cells of the rat was investigated using the peroxydase-antiperoxydase (PAP) technique. A rabbit antiserum against purified rat plasma transcortin was used as the primary antiserum. Transcortin-like (CBG-like) immunoreactive products were found in the cytoplasma of certain cells in the anterior pituitary, but not in the intermediate lobe and weakly in the posterior pituitary. It is postulated that the CBG-like molecules participate in the cellular uptake process of corticosterone, thereby m~uIating the feedback signal of this steroid on pituitary function.

Intrinsic expression of transcortin in neural cells of the mouse brain: a histochemical and molecular study

“…The expression of CBG in the brain has been reported before by various authors in rats (de Kloet et al, 1984;Perrot-Applanat et al, 1984;Möpert et al, 2006;Jirikowski et al, 2007) and humans (Sivukhina et al, 2006 coexpression of the steroid-binding globulin with the classical neuroendocrine peptides within the rat hypothalamus as well as intrinsic synthesis of Cbg mRNA in various brain regions. Here, we extend the data obtained previously by the use of a mouse model in which expression of the Cbg gene is totally abolished in each tissue of the animal.…”

Expression of corticosteroid binding globulin in the rat central nervous system

“…CBG has been observed with immunocytochemistry in some of the endocrine cells of the anterior pituitary lobe and with biochemical methods in brain tissue (De Moor et al, 1962;De Kloet et al, 1984). Due to their lipophilic nature, steroid hormones are capable of crossing the blood brain barrier and stress-related behavioural changes are clearly modulated by adrenal steroids.…”

Selective Control by Corticosterone of Serotonin Receptor Capacity in Raphe-Hippocampal System

“…This role of CBG may be apparent in the kidney or in the circumventricular organs. A similar line of reasoning has been followed with regard to the GR in the anterior pi tuitary, where the actual intracellular localization of CBG has been verified immunocytochemically [18].…”