Intracellular calcium redistribution during mating in Chlamydomonas reinhardii

Ethanol stimulates phospholipid turnover and inositol 1,4,5-trisphosphate production in Chlamydomonas eugametos gametes

“…Although conclusive evidence has yet to be provided, calcium has also been implicated in the sexual activation of C. reinhardtii gametes (Claes 1980;Snell et al 1982), and in two reports, evidence was presented that calcium is released from intracellular stores (Bloodgood and Levin 1983;Kaska et al 1985) in accordance with the role proposed here for Ins(1,4,5)P3. Nonetheless, calcium is only one of the intracellular signals that have been invoked, for convincing evidence already exists that adenosine 2':3'-cyclic monophosphate plays a role in the sexual activation of both Chlamydomonas species (Pijst et al 1984;Pasquale and Goodenough 1987;Kooijman et al 1990).…”

“…Evidence has been presented for two intracellular signals i.e. adenosine 2': 3'-cyclic monophosphate (Pijst et al 1984;Pasquale and Goodenough 1987;Kooijman et al * To whom correspondence should be addressed Abbreviations: Ins(1,4,5)P3=inositol 1,4,5-trisphosphate; PtdA= phosphatidic acid: PtdIns=phosphatidylinositol; PtdIns(4)P= phosphatidylinositol 4-phosphate; PtdIns(4,5)Pz=phosphatidyli-nositol 4,5-bisphosphate 1990) and calcium (Claes 1980;Snell et al 1982; Bloodgood and Levin t983; Kaska et al 1985). When a membrane-permeable analogue of adenosine 2':Y-cyclic monophosphate is added to gametes, or when gametes are treated with compounds that may be expected to raise the intracellular calcium level, mating structures are activated (Pasquale and Goodenough 1987;Kooijman et al 1990;Schuring et al 1990).…”

Characterisation and cloning of a calmodulin-like domain protein kinase from Chlamydomonas moewusii (Gerloff )

“…The InsP 3 can then induce the release of calcium from internal stores by binding to the InsP 3 receptor (Berridge 1993). Potential InsP 3 -sensitive stores of calcium have been characterised in Chlamydomonas (Kaska et al 1985;Siderius et al 1996).…”

Polyphosphoinositol lipids in Chlamydomonas eugametos gametes

“…Firstly, the presence of PtdInsP2 ) and its rapid turnover have now been established for sexually competent gametes. Secondly, during gamete pairing, calcium is released from stores within the cell (Bloodgood and Levin 1983; Kaska et al 1985), and artificially raising the calcium level in non-pairing gametes sexually activates them (Schuring et al 1990). In particular, treating permeabilized cells with InsP 3 activates their mating structures.…”