Intracellular calcium and pH conditions of cultured cells infected with Eimeria bovis or E. separata

Behrendt, Jan Hillern; Milde, Helga; Weber, Wolf-Michael; Kowalik, Sabine; Zahner, Horst; Bürger, Harald; Clauss, Wolfgang

doi:10.1007/s004360050046

Cited by 7 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, in their review, Saliba and Kirk (2000) discussed the possibility that the apparent difference in [Ca 2+ ] in the parasitophorous vacuole (PV) and host cell cytosol reflects other differences in composition between the two compartments. This is confirmed by findings of Behrendt et al (2000) who found the same [Ca 2+ ] in the host cell cytosol, the PV and the Fig. 5a, b The g t , Cl ) fluxes (J Cl ) and mannitol fluxes (J H ) of T. gondii-infected (5 and 15 h p.i.)…”

Section: Discussionsupporting

confidence: 91%

Toxoplasma gondii : changes of transepithelial ion transport in infected HT29/B6 cell monolayers

Kowalik

Clauss

Zahner

2004

Parasitology Research

Self Cite

View full text Add to dashboard Cite

An in vitro system was established to study the effect of coccidian parasites on ion transport systems in epithelial tissues using HT29/B6, a human colon carcinoma cell line, and Toxoplasma gondii as a model parasite. Ion transport was measured in perfusion chambers 5, 10 and 15 h post-infection using monolayers in which approximately 30% of the cells were parasitized. The infection had rapid effects on the conductance and unidirectional chloride fluxes of infected cell monolayers, which were two to three times higher than those of uninfected HT29/B6 cell monolayers throughout the observation period. However, the chloride net fluxes and short-circuit current were unaffected by the parasites, while the decrease of chloride seromucosal fluxes and conductance after addition of bumetanide were affected by the infection. The unidirectional mannitol fluxes, which correspond with water motion through paracellular pathways, were increased in infected HT29/B6 cell monolayers.

show abstract

Section: Discussionsupporting

confidence: 91%

Toxoplasma gondii : changes of transepithelial ion transport in infected HT29/B6 cell monolayers

Kowalik

Clauss

Zahner

2004

Parasitology Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…In fact, E. bovis sporozoite egress from cells may still occur later on observed it 8 days after infection], and can be provoked by mechanical irritation of the cell (e.g. during patch clamp experiments; Behrendt et al, unpubl.; see also Behrendt et al 2000). However, it happened generally only with parasites of the original slender type.…”

Section: Discussionmentioning

confidence: 92%

“…Thus, the quick development and expansion of the vacuole should be associated with an enhanced synthesis and secretion/exocytosis of such organellar products. Independent of these questions, however, the E. bovis-infected VERO cell with a large parasitophorous vacuole represents an interesting experimental tool, which has been recently used for permeability studies and analyses of Ca 2+ content and pH conditions in the various compartments of the infected cell (Behrendt et al 2000). Development of the parasite to mature schizont took place only in cells of bovine origin.…”

Section: Discussionmentioning

confidence: 99%

Development of Eimeria bovis in vitro: suitability of several bovine, human and porcine endothelial cell lines, bovine fetal gastrointestinal, Madin–Darby bovine kidney (MDBK) and African green monkey kidney (VERO) cells

et al. 2001

View full text Add to dashboard Cite

Several bovine, human and porcine endothelial cell lines, bovine fetal gastrointestinal cells (BFGC), Madin-Darby bovine kidney (MDBK) and African green monkey kidney (VERO) cells were exposed in vitro to sporozoites of Eimeria bovis. Parasites invaded all cells used and changed their shape to more stumpy forms within 12 h. Sporozoites left their host cells and invaded new ones frequently within the first 12 h post-infection. Further development took place only in bovine cells, although parasites survived in the other cells for at least 3 weeks. Within the non-bovine cells, conspicuously enlarged parasitophorous vacuoles developed in VERO cells and reached a diameter of 15-20 microm. The best development to first generation schizonts with regard both to time required to mature, to schizont size and to merozoite yields was observed in BFGC, followed by bovine umbilical vein and bovine spleen lymphatic endothelial cells. MDBK cells were less suitable. The life cycle was completed (development of oocysts) only occasionally in BFGC. Results are considered under several aspects. Thus, infected VERO cells may represent a suitable tool for studying the parasitophorous vacuole, while infected endothelial cells represent a system quite narrow to the in vivo situation and should allow basic studies on parasite/host cell interactions and BFGC can be used for the mass production of E. bovis first generation merozoites.

show abstract

“…To the best of our knowledge, this is the first study to simultaneously measure force, [Ca 2ϩ ] i , and pH i in the portal vein. Previous studies in rat mesenteric vessels have shown the usefulness and validity of this approach (5), but few studies in any other tissue have been reported (3,8,20). The fluorescent spectra of the two indicators and the filters used were such that they did not interfere with each other.…”

Section: Discussionmentioning

confidence: 99%

pH-induced changes in calcium: functional consequences and mechanisms of action in guinea pig portal vein

Smith

Eisner

Wray

2002

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

The effects of changing extracellular (pHo) and intracellular pH (pHi) on force and the mechanisms involved in the guinea pig portal vein were investigated to better understand the control of tone in this vessel. When pHo was altered, the effects on force and calcium were the same irrespective of whether force had been produced spontaneously by high-K depolarization or by norepinephrine; alkalinization increased tone, and acidification reduced it. Because pHo changes also lead to changes in pHi, we determined whether the effects on force could be explained by these induced pHi changes. It was found, however, that only with spontaneous activity did intracellular alkalinization increase force. In depolarized preparations, force was decreased, and, with norepinephrine, force was initially decreased and then increased. Thus the effects of pHo cannot be explained solely by changes in pHi. The role of the sarcoplasmic reticulum (SR) and surface membrane Ca2+-ATPase on the mechanism were investigated and shown not to be involved. Therefore, it is concluded that both pHo and pHi can have powerful modulatory effects on portal vein tone, that these effects are not identical, and that they are likely to be due to effects of pH on ion channels rather than the SR or plasma membrane Ca2+-ATPase.

show abstract

Intracellular calcium and pH conditions of cultured cells infected with Eimeria bovis or E. separata

Cited by 7 publications

References 21 publications

Toxoplasma gondii : changes of transepithelial ion transport in infected HT29/B6 cell monolayers

Toxoplasma gondii : changes of transepithelial ion transport in infected HT29/B6 cell monolayers

Development of Eimeria bovis in vitro: suitability of several bovine, human and porcine endothelial cell lines, bovine fetal gastrointestinal, Madin–Darby bovine kidney (MDBK) and African green monkey kidney (VERO) cells

pH-induced changes in calcium: functional consequences and mechanisms of action in guinea pig portal vein

Contact Info

Product

Resources

About