Intracellular Bottom‐up Synthesis of Ultrasmall CuS Nanodots in Cancer Cells for Simultaneous Photothermal Therapy and COX‐2 Inactivation

Abstract

The clinical application of photothermal therapy (PTT) is limited by the accuracy of thermal damage and the risk of tumor metastasis and relapse induced by hyperthermia‐related inflammation. Intracellular bottom‐up synthesis (iBuS) of CuS nanoparticles from small‐molecule precursors inside tumor cells triggered by tumor specific stimuli is a promising strategy to enhance the precision of PTT treatment and reduce the risk of nondegradable metal nanoparticles. Herein, monolocking nanoparticles (MLNPs) with Cu‐meloxicam complexes encapsulated by human serum albumin (HSA) are reported, which efficiently form CuS nanodots via the elevated concentration of endogenous H2S inside tumor cells and meanwhile release meloxicam for anti‐inflammatory effects. The intracellular bottom‐up fabrication of CuS nanodots is directly visualized by TEM. An enhanced PTT effect is observed with 4T1 cells caused by additional meloxicam‐induced inactivation of the COX‐2 enzyme. After systemic administration, MLNPs completely ablate tumors under laser exposure, simultaneously inhibiting the inflammation induced by photothermal damage, and can be cleared via the kidney into urine. This strategy provides a new route for activated multimodal therapy, which could be applicable to precisely combat cancer.

An adenosine triphosphate-responsive metal–organic framework decorated with palladium nanosheets for synergistic tri-modal therapy

“…Various nanoplatforms have been designed to realize multi-modal synergistic therapy, [7][8][9][10] among which dual-modal synergistic therapy, such as chemotherapy/PTT, chemotherapy/CDT and CDT/PTT, has been wildly reported, while synergistic tri-modal synergistic therapy, such as chemotherapy/CDT/PTT, is still at an early age due to the difficulty in designing a more complex system. Two-dimensional (2D) nanomaterials, such as graphene, 11-14 metal oxides and transitional metal chalcogenides, [15][16][17][18][19] have attracted much attention in biological applications due to their unique optical properties and excellent biocompatibility. Specifically, palladium nanosheets (Pd NSs), as typical 2D materials, exhibit several unique characteristics, such as strong near-infrared (NIR) light absorption and high photothermal conversion efficiency, which have potential applications in photothermal therapy.…”

“…Two-dimensional (2D) nanomaterials, such as graphene, 11–14 metal oxides and transitional metal chalcogenides, 15–19 have attracted much attention in biological applications due to their unique optical properties and excellent biocompatibility. Specifically, palladium nanosheets (Pd NSs), as typical 2D materials, exhibit several unique characteristics, such as strong near-infrared (NIR) light absorption and high photothermal conversion efficiency, which have potential applications in photothermal therapy.…”

H2S‐Responsive Small‐Molecule Nanocarriers for Drug Delivery to Colorectal Tumors

“…[13][14][15][16] Several studies have demonstrated the superiority of H 2 S as an internal stimulus for drug delivery. [17][18][19][20][21][22][23][24][25][26][27][28] For instance, copper sulfide (CuS) formation via the reaction between H 2 S and copper ions was utilized to release copper-coordinated doxorubicin (DOX) from protein nanocages. [29] In situ formation of CuS was used for activating photothermal therapy and triggering drug release from liposomes [30] or metal-organic framework.…”

An Ecoconceptual Synthesis Process of Silver Nanoparticles Integrating the Valorization of a Food Waste: The Peanut Shell

“…In this general theme, the design of nanoparticles is an extremely developed research activity, in order to satisfy numerous applications [4] [5]. The field of use of nanoparticles seems unlimited, from nanomedicine [6] [7] to agriculture elaboration protocols have evolved a lot within the classical top-down [14] [15] or bottom-up approaches [16] [17]. Currently, processes are moving towards more eco-friendly approaches [18] [19] that can be part of sustainable development [20] [21].…”