Intracellular Acidification Is Associated with Changes in Free Cytosolic Calcium and Inhibition of Action Potentials in Rat Trigeminal Ganglion

Hwang, Sun-Mi; Koo, Na‐Youn; Jin, Meihong; Davies, AN; Chun, Gae-Sig; Choi, Se‐Young; Kim, Joong Soo; Park, Kyungpyo

doi:10.1074/jbc.m109.090951

Cited by 33 publications

(27 citation statements)

References 45 publications

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“…Our laboratory has previously reported an endogenous mechanism of intracellular acidification that is mediated by PMCA, which involves a rise in intracellular Ca 2ϩ induced by glutamate or capsaicin in sensory neu- rons (26). However, we observed no interaction between STCH and the acidifying transporter PMCA.…”

Section: Discussioncontrasting

confidence: 51%

Chaperone Stress 70 Protein (STCH) Binds and Regulates Two Acid/Base Transporters NBCe1-B and NHE1 *

Bae

Koo

Namkoong

et al. 2013

Journal of Biological Chemistry

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Background: Regulation of intracellular pH is critical for cellular homeostasis. Results: Stress 70 protein chaperone (STCH) interacts with pH regulating transporters NBCe1-B and NHE1 and modulates their functional expression. Conclusion: STCH chaperone dynamically regulates intracellular pH through site-specific interactions with ion transporters. Significance: These novel STCH interactions provide a mechanism for intracellular pH regulation in response to homeostatic stress.

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Section: Discussioncontrasting

confidence: 51%

Chaperone Stress 70 Protein (STCH) Binds and Regulates Two Acid/Base Transporters NBCe1-B and NHE1 *

Bae

Koo

Namkoong

et al. 2013

Journal of Biological Chemistry

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“…Thus NBCe1-B/C is likely to be the major NCBT in these cells. Furthermore, the frequency of action potential firing in response to current-injection in these cells is reduced by intracellular acidification and by DIDS treatment (408). Taken together, these data indicate that NBCe1-B/C mediates an uptake of HCO 3 Ϫ that counters the dampening effect of intracellular acidification (408), FIGURE 24A pe and thereby plays an important role in maintaining excitability in these neurons.…”

Section: Iv) Peripheral Nervous System A) Neuronal Excitabilitymentioning

confidence: 94%

“…In primary cultures of neurons from the rat trigeminal ganglion, application of anti-NBCe1-B/C siRNA results in a ϳ50% reduction of NBCe1-B/C protein abundance and, following an NH 4 ϩ prepulse, causes a near total elimination of HCO 3 Ϫ -dependent acid-extrusion in these cells (408). Thus NBCe1-B/C is likely to be the major NCBT in these cells.…”

Section: Iv) Peripheral Nervous System A) Neuronal Excitabilitymentioning

confidence: 97%

The Divergence, Actions, Roles, and Relatives of Sodium-Coupled Bicarbonate Transporters

Parker

Boron

2013

Physiological Reviews

246

262

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The mammalian Slc4 (Solute carrier 4) family of transporters is a functionally diverse group of 10 multi-spanning membrane proteins that includes three Cl-HCO3 exchangers (AE1-3), five Na(+)-coupled HCO3(-) transporters (NCBTs), and two other unusual members (AE4, BTR1). In this review, we mainly focus on the five mammalian NCBTs-NBCe1, NBCe2, NBCn1, NDCBE, and NBCn2. Each plays a specialized role in maintaining intracellular pH and, by contributing to the movement of HCO3(-) across epithelia, in maintaining whole-body pH and otherwise contributing to epithelial transport. Disruptions involving NCBT genes are linked to blindness, deafness, proximal renal tubular acidosis, mental retardation, and epilepsy. We also review AE1-3, AE4, and BTR1, addressing their relevance to the study of NCBTs. This review draws together recent advances in our understanding of the phylogenetic origins and physiological relevance of NCBTs and their progenitors. Underlying these advances is progress in such diverse disciplines as physiology, molecular biology, genetics, immunocytochemistry, proteomics, and structural biology. This review highlights the key similarities and differences between individual NCBTs and the genes that encode them and also clarifies the sometimes confusing NCBT nomenclature.

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“…It less effectively inhibits NHE4 (IC50 P10 lM). EIPA is commonly used at a concentration of 5-10 lM to inhibit cellular NHE activity [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of the Na+/H+ antiporter induces cell death in TF-1 erythroleukemia cells stimulated by the stem cell factor

et al. 2015

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Intracellular Acidification Is Associated with Changes in Free Cytosolic Calcium and Inhibition of Action Potentials in Rat Trigeminal Ganglion

Cited by 33 publications

References 45 publications

Chaperone Stress 70 Protein (STCH) Binds and Regulates Two Acid/Base Transporters NBCe1-B and NHE1 *

Chaperone Stress 70 Protein (STCH) Binds and Regulates Two Acid/Base Transporters NBCe1-B and NHE1 *

The Divergence, Actions, Roles, and Relatives of Sodium-Coupled Bicarbonate Transporters

Inhibition of the Na+/H+ antiporter induces cell death in TF-1 erythroleukemia cells stimulated by the stem cell factor

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