Intracellul�re Bewegungsorganellen der Myxobakterien

Electron microscopic observations of thin sections of Myxococcus xanthus vegetative cells revealed the presence of cytoplasmic bundles of 4- to 5-nm-diameter filaments running longtitudinally below the cell membrane and terminating in association with the envelope near one pole. Part of each bundle demonstrated a herringbone-like periodicity (approximately 12-nm spacing). This structure was observed in cells from shake cultures and in gliding cells fixed by several methods. It is proposed that the structure may be attached to the envelope near both poles in gliding cells and that the motive force for motility may be provided by its contraction and relaxation. In one of four nongliding mutants examined, the periodicity was indistinct or lacking. In this mutant another structure, comprised of linearly arrayed beads, was observed in association with the filamentous bundle. Another structure, characterized by major, transverse bands (approximately 34 nm apart), occurred in patches that may traverse the diameter of the wild-type cells in which the structure was observed.

Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Intracellular, periodic structures in the gliding bacterium Myxococcus xanthus

Burchard¹,

Burchard²,

Kloetzel³

1977

“…Intracellular filaments or microtubules observed in Myxococcus spp. were proposed as the gliding organelles (17). We have not detected similar structures in M. xanthus nor in C. columnaris.…”

contrasting

confidence: 60%

Surface Structure of Gliding Bacteria After Freeze-Etching

Burchard

Brown

1973

“…MATERIALS AND METHODS Strains. M. xanthus XK is the same strain pteviously used by Schmidt-Lorenz and Kiihlwein (20). The origin of Cystobacter sp.…”

mentioning

confidence: 99%

Comparison of spontaneous, UV-induced, and nitrosoguanidine-induced mutability to drug resistance in myxobacteria

Grimm¹

1978

The UV survival curves of different strains ofmyxobacteria exhibited shoulders; in the cam of Polyangium luteum, an unusual double shoulder appeared. Repair inhibitors like acriflavine, caffeine, and coumarin reduced the survival of UVirradiated cells if the drugs were incorporated in the post-irradiation plating medium. The shoulders were reduced, but the final inactivation slopes were not affected by the repair inhibitors. Those strains that were resistant to UV were also more resistant to being killed by nitrosoguanidine. A variety of drug-resistant mutants occurred. The spontaneous mutation frequencies to drug resistance varied with the drug and the strain used. Drug-resistant mutants were inducible by UV irradiation and nitrosoguanidine. The UV mutability of Myxococcus xanthus was high compared to Cystobacter sp. However, the nitrosoguanidine mutability of M. xanthus was low compared to the other strains.