Intracavernous Pressure as an Experimental Index in a Rat Model for the Evaluation of Penile Erection

Chen, Kuang-Kuo; Chan, Julie Y.H.; Chang, Luke S.; Chen, Ming-Tsun; Chan, Samuel H.H.

doi:10.1016/s0022-5347(17)37500-6

Cited by 70 publications

(50 citation statements)

References 16 publications

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“…In previous studies, differences in the amplitude and time course of erections in rats, induced by electrical nerve stimulation as differentiated from those obtained by intracavernous injection, might be explained by this concept. That is, electrical nerve stimulation produces an increase in intracavernous pressure to near maximal levels within seconds 18 compared with the much slower, blunted pro®le over 20 ± 25 sec that occurs with direct corporal injection. 19 In addition, Garban et al observed that the maximum intracavernous pressure elicited by direct injection into the corpus cavernosum is % 25% lower than that caused by electrical nerve stimulation.…”

Section: Discussionmentioning

confidence: 99%

Pre-penile arteries are dominant in the regulation of penile vascular resistance in the rat

et al. 2000

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The amount of blood¯ow into the penis that will produce an erection is dependent on the sum of in¯ow resistance from the feeder arteries, arterioles and the intra-penile vasculature. In the present study, our objective was to determine quantitatively the contribution to in¯ow resistance of these different components of the rat penile vasculature. Using methods developed previously, we determined the resistance properties of the isolated perfused whole penis in situ, both in an intact system and after serial transactions of the vessels. These cuts eliminated progressively larger distal segments of the vascular bed. Perfusion pressures were recorded at different¯ow rates (0.5 ± 3 mlaminakg body weight) under conditions of maximal dilatation and maximal vasoconstriction induced by methoxamine (MXA, 40 mgaml).Regardless of the level of vascular tone, the pudendal artery contributes approximately 70% of the total resistance of the penile vasculature. In contrast, the vasculature within the penis (tip, shaft, crus) contributes only about one quarter of the resistance. Penile arterial in¯ow resistance properties both at maximal vasodilation and maximal a 1 -adrenergic constriction are dominated by the extra-penile vasculature in the rat. The implications of these ®ndings are that alterations in the pudendal-artery (eg vasodilation, vasoconstriction, stenosis) would have primary control of arterial in¯ow and suggest an important role for pharmacological agents which can promote a more generalized vasodilation (eg phosphodiesterase inhibitors) in contrast to selective corpus cavernosal agents.

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Section: Discussionmentioning

confidence: 99%

Pre-penile arteries are dominant in the regulation of penile vascular resistance in the rat

et al. 2000

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“…4 In the experiments, 21 adult male Sprague-Dawley rats (300-500 g) were anesthetized with tiletamine (Zoletil; 100 mg kg À1 ) and xylazine (20 mg kg À1 ) i.p., and anesthesia was maintained during the experimental protocol by continuous i.v. infusion with pentobarbital sodium at 2 mg kg À1 h À1 .…”

Section: Animal Experiments To Verify the Proposed Methodsmentioning

confidence: 99%

“…4,18 In our research we used rapid papaverine administration to induce the physical pressure of the rigid erection phase in rats. Papaverine hydrochloride (0.4 mg in 0.1 ml) was administered intracavernously via the ICP recording needle.…”

Section: Icp Analysis For Erection Phase Identification W-r Han Et Almentioning

confidence: 99%

“…The changes in the ICP and the different phases of an erection are pivotal in determining the ability to produce and maintain a rigid penile erection. 3,4 The ICP corresponds to the vascular pressure recorded within the cavernous and spongious corporal bodies of the penis. The reported definition of the erectile phases in a rat model 5 is similar to those used in previous researches in monkeys.…”

Section: Introductionmentioning

confidence: 99%

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Intracavernosal pressure characteristics analyzed by power spectral density for identifying the phase of penile erection

Wr¹,

Cy²,

Pc³

et al. 2009

Int J Impot Res

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Knowledge of the time course of penile erection is very important to understanding erection physiology. The changes in the intracavernosal pressure (ICP) and the different phases of an erection are pivotal to the ability to produce and maintain a rigid penile erection. This study investigated an objective and low-invasiveness method for identifying different erection phases based on an innovative ICP analysis technique. Blood infuses into the corpora cavernosa and causes the ICP to increase. The ICP usually exhibits tiny oscillations at the frequency of the heartbeat when it increases from diastole to systole. The characteristic oscillation amplitudes corresponding to the period when the full and rigid erection phases begin can be extracted by power spectral density analysis. The reliability and accuracy of the proposed method was verified by the Bland-Altman graphs indicating a good agreement with the existing method that compares the ICP with the arterial pressure. Moreover, all of the intraclass correlation coefficient values were close to 1.00, with the lower limit of the 95% confidence interval exceeding 0.75. The described novel objective and low-invasiveness method can therefore be used for identifying the full and rigid erection phases of the penis in urological investigations during different erection phases.

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“…Many experimental animals have been employed in in vivo studies of penile erection, such as dogs, monkeys, cats, rats and rabbits (Chen et al, 1992;Domer et al, 1978;Lin et al, 1985;Lue et al, 1983;Stacle et al, 1988). Lin & Lin (1996) reported that the e ects of vasoactive drugs on the rabbit corpus cavernosum are similar to those in humans; thus the rabbit model is a suitable alternative for further physiological and pharmacological studies of penile erection.…”

Section: Introductionmentioning

confidence: 99%

Relaxation of corpus cavernosum and raised intracavernous pressure by berberine in rabbit

Chiou

Chen²,

Chen

1998

British J Pharmacology

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1 In the present study, we have investigated the e ect of berberine in rabbit isolated corpus cavernosum and measured the intracavernous pressure (ICP) change after intracavernosal injection of berberine in rabbit. 2 Berberine alone suppressed the basal tone and induced a concentration (0.1 ± 100 mM)-dependent relaxation in phenylephrine (PE)-precontracted corpus cavernosum. 3 Tetrodotoxin (0.1 and 1 mM) treatment had no signi®cant e ect on the berberine-induced relaxation. Phentolamine (1 and 10 mM), propranolol (1 and 3 mM) and atropine (1 and 3 mM) were also without e ect. These results suggest that berberine might cause relaxation of the cavernosal strip by direct action on the corpus cavernosum, not by a neuronal e ect. Furthermore, muscarinicand b-adrenoceptors were not involved. 4 Berberine-induced relaxations were signi®cantly reduced by endothelium removal and by exposure to L-N G -nitro arginine methyl ester (0.1 and 0.3 mM), but not indomethacin (30 mM). 5 In endothelium-deprived corpus cavernosal tissues, berberine-induced relaxations were signi®cantly reduced in high K + medium (KCl=60 mM), by charybdotoxin (ChTX) and 4-aminopyridine (4-AP) but not by glibenclamide and apamin. 6 After intracavernous injection of berberine (1, 2, 3 and 5 mg kg 71 ), the ICP rose from 12.7+3.6 to 13.2+5.4, 25.3+6.1, 46.5+8.2, and 63.4+10.2 mmHg, respectively. The duration of tumescence ranged from 11.5 ± 43.7 min. 7 The results show that berberine possesses a relaxant e ect on rabbit corpus cavernosal tissues which is attributable to both endothelium-dependent and-independent properties. While the former component is apparently due to the release of NO from sinusoidal endothelium, the endotheliumindependent mechanism involved in berberine relaxation is probably linked to ChTX-and 4-APsensitive K + channel activation in the cavernosal vasculature.

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Intracavernous Pressure as an Experimental Index in a Rat Model for the Evaluation of Penile Erection

Cited by 70 publications

References 16 publications

Pre-penile arteries are dominant in the regulation of penile vascular resistance in the rat

Pre-penile arteries are dominant in the regulation of penile vascular resistance in the rat

Intracavernosal pressure characteristics analyzed by power spectral density for identifying the phase of penile erection

Relaxation of corpus cavernosum and raised intracavernous pressure by berberine in rabbit

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