Intra vitam staining with methylene blue

Wilson, J. Gordon

doi:10.1002/ar.1090040705

Cited by 17 publications

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“…Since the MB coloration of the tissue develops upon exposure to the air/oxygen [21], the evaluation of staining was performed 5 minutes after the removal of the IOM from the TAM. The MB staining of the nerves was evaluated through direct visualization by the anatomist and both anaesthetists.…”

Section: Four-point Tap Technique: Injection Pointsmentioning

confidence: 99%

Development of four-point transversus abdominis plane block technique in pigs. A pilot cadaveric study

Sreden,

Brankovič,

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et al. 2024

Preprint

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Background This prospective pilot cadaveric study aimed to develop an ultrasound-guided transversus abdominis plane (TAP) block technique that desensitizes cranial and mid-abdominal wall in pigs. Methods In phase I, the ultrasound anatomy of the abdominal wall musculature was examined on three pig cadavers and the ultrasound localization of the needle in the corresponding interfascial plane was practised. In phase II, a three-point TAP injection was performed in three freshly euthanized cadavers. A 1% methylene blue solution (0.3 mL/kg per injection point) was injected between the transversus abdominis and internal oblique muscle. In phase III, methylene blue solution was injected at four points (0.2 mL/kg per injection point) in four anaesthetized pigs prior to euthanasia. Positive nerve staining was defined as continuous staining of at least 1 cm of the nerve length. Binary variables (positive/negative) were used for nerve staining assessment. Results The four-point technique with a lower injected volume stained more nerves than the three-point technique with a higher injected volume. In phase III, 69% of the observed nerves from the eighth-last thoracic to the third lumbar nerve were stained. The nerves in the centre were stained with a higher success rate, while the eighth-last thoracic and the second lumbar nerve were stained with less success (1/8 and 3/8, respectively). The third lumbar nerve was not reached by any injection. Conclusions The four-point TAP technique could be used as part of a multimodal analgesia approach for cranial and middle abdominal surgery in pigs, but live animal studies are needed to evaluate the clinical applicability and efficacy of desensitisation.

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Section: Four-point Tap Technique: Injection Pointsmentioning

confidence: 99%

Development of four-point transversus abdominis plane block technique in pigs. A pilot cadaveric study

Sreden,

Brankovič,

Tratar

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Either the dye or its colourless and more hydrophobic leuco compound may be used; advantages are claimed for both [138,144]. Adequate oxygenation is necessary in order to see the stained cells in the fresh state, but the dye can be immobilised in situ by a suitable precipitating anion; ammonium molybdate is traditional [145], but advantages are claimed for recent variants of the technique that include treating the stained preparations with glutaraldehyde and PMA [140,146,147].…”

Section: Vital Staining and Neuroanatomical Tracingmentioning

confidence: 99%

Dyes and other colorants in microtechnique and biomedical research

Kiernan

2006

Coloration Technology

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“…For the demonstration of the nerve fibrils and ganglion cells within the node we employed a vital stain of methylene-blue, following for the Keith-Flack node the same technique as Wilson (15,16) applied to the bundle of His. In the course of the work we also had the advantage of having Dr. Wilson personally demonstrate the procedure to us.…”

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confidence: 99%

Nerve Fibrils in the Sino-Auricular Node

Oppenheimer

1912

Journal of Experimental Medicine

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PLATES 65-67.The purpose of the following paper is to describe nervous structures found within the sino-auricular node by the use of a vital stain of methylene-blue. Since the original description by Keith and Flack ( I ) in 19o7, considerable interest has attached to the sinoauricular node; and its normal histology, its function, and' its condition in pathological hearts have been actively investigated. As regards its function, the experimental evidence points strongly to it as the primum movens of the heart (2, 3, 4), although a very few observers apparently are not inclined to accept this view as yet (5, 6, 7). Its relation to certain clinical conditions, more especially to auricular fibrillation (pulsus irregularis perpetuus), is being studied, but so far the reports on the hearts examined consist in the main of descriptions of the findings in and around the node without any assertion that the changes in the node account for the clinical condition.Pathological changes of the intranodal nervous structures have not received much attention; indeed thus far no one has described the finer nervo~s elements occurring normally within the node. In addition to the numerous studies of the general cardiac nervous system, both intra-and extracardiac, which have extended over many years, three recent important contributions to the nervous structures of the auriculo-ventricular system have been made; namely, those of Wilson (8), De Witt (9), and Engel (IO). Wilson was the first to demonstrate by means ,of a vital stain not only

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Intra vitam staining with methylene blue

Cited by 17 publications

References 0 publications

Development of four-point transversus abdominis plane block technique in pigs. A pilot cadaveric study

Development of four-point transversus abdominis plane block technique in pigs. A pilot cadaveric study

Dyes and other colorants in microtechnique and biomedical research

Nerve Fibrils in the Sino-Auricular Node

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