Intra-varietal variability and genetic relationships among the homonymic East Adriatic olive ( Olea europaea L.) varieties

Lazović, Biljana; Klepo, Tatjana; Adakalić, Mirjana; Šatović, Zlatko; Arbeiter, Alenka Baruca; Hladnik, Matjaž; Strikić, Frane; Liber, Zlatko; Bandelj, Dunja

doi:10.1016/j.scienta.2018.02.053

Cited by 15 publications

(20 citation statements)

References 52 publications

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“…The VAL2 coded olive tree has the lowest genetic similarity (0.619) to 'Žutica' . For this tree, Lazović et al (2018a) suggest that the most likely is a putative seedling of 'Žutica' . This genetically most distant sample was different from the group of identical microsatellite profiles on 6 loci (8 alleles).…”

Section: Microsatellites Analysismentioning

confidence: 94%

“…The average number of alleles per each locus was 3.75, ranging from 2 at loci DCA14 and EMO3 to 7 at loci DCA9 and DCA11. These values are lower than the number of alleles detected by Lopes et al (2004) and , but slightly higher than those detected by Muzzalupo et al (2009) and Lazović et al (2018a). The information value of the locus also depends on the frequency of alleles, therefore the number of effective alleles was calculated, which differed from the actual number of alleles, and on average it was 2.21 per locus.…”

Section: Microsatellites Analysismentioning

confidence: 96%

“…Those are advantages that could be implemented in the future olive growing and spreading of such clones. Previous research on intra-varietal variability in 'Žutica' was conducted by Lazović et al (2002Lazović et al ( , 2016Lazović et al ( , 2018a. RAPD and SSR polymorphism was revealed, but the morphological properties of leaf, inflorescences, fruit and endocarp of the same clones were not analyzed.…”

Section: Introductionmentioning

confidence: 99%

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Morphological and microsatellite analysis of the ancient Montenegrin olive variety ‘Žutica’ revealed different clones

Adakalić

Lazović

Arbeiter

et al. 2020

AAS

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<p>The ‘Žutica’ represents the most common Montenegrin olive varieties mainly used for the production of olive oil and green and black fruit canning. Traditionally, the olive plants have been propagated vegetatively, and a small level of genetic polymorphism is expected among clones of the same variety. This topic was only partially studied in the Montenegrin olive ‘Žutica’. Therefore, this study aimed to determine intra-varietal genetic variability in twenty-three ‘Žutica’ trees selected in situ, analyzing the variability of morphological traits and microsatellites. The Principal Component Analyses (PCA) with six axes explains the total cumulative variance of 91.3 %, with fruit and endocarp traits in the first three PC. The unweighted pair group method with arithmetic mean of twenty morphological traits grouped ‘Žutica’ trees into two clusters and five independent trees. Nine microsatellite primers amplified 31 fragments of which 22 were polymorphic and enabled the detection of nine different microsatellite profiles (potential different clones). A comparison of dendrogram groups based on morphological and microsatellite markers showed low cophenetic values in the determination of intra-varietal variability. The results showed that the old variety ‘Žutica’, from a relatively small geographic region, has a variable genetic base, which could be used in the selection of superior clones.</p>

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Section: Microsatellites Analysismentioning

confidence: 94%

Section: Microsatellites Analysismentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological and microsatellite analysis of the ancient Montenegrin olive variety ‘Žutica’ revealed different clones

Adakalić

Lazović

Arbeiter

et al. 2020

AAS

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“…The world’s olive germplasm contains more than 2629 different varieties, with many local varieties and ecotypes contributing to this richness; in Italy, about 800 cultivars are present [ 22 , 23 ]. The cultivar Bianchera is an autochthonous variety of Trieste, a city located in the eastern part of northern Italy, but it is also cultivated in the Slovenian and Croatian peninsula of Istria, where it is named Belica (Slovenia) and Bjelica (Croatia) [ 24 , 25 ]. Its characteristic is to be resistant to low temperatures and strong wind, typical of the city of Trieste, and since phenols are usually produced by the plant for protection against stress, in the present work, a complete characterisation of the phenols found in olive oil, olive leaves and pomace was performed.…”

Section: Introductionmentioning

confidence: 99%

Oleocanthal Quantification Using 1H NMR Spectroscopy and Polyphenols HPLC Analysis of Olive Oil from the Bianchera/Belica Cultivar

et al. 2021

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The cultivar Bianchera is an autochthonous variety from the eastern part of northern Italy, but it is also cultivated in the Slovenian and Croatian peninsula of Istria where it is named Belica (Slovenia) and Bjelica (Croatia). The properties of oleocanthal, a natural anti-inflammatory ibuprofen-like compound found in commercial monocultivar extra virgin olive oils, were determined by means of both quantitative 1H NMR (qNMR) and HPLC analyses, where qNMR was identified as a rapid and reliable method for determining the oleocanthal content. The total phenolic content (TPC) was determined by means of the Folin–Ciocalteau method and the major phenols present in the olive oils were also quantified by means of HPLC analyses. All these analyses confirmed that the cultivar Bianchera was very rich in polyphenols and satisfied the health claim provided by the EU Commission Regulation on the polyphenols content of olive oils and their beneficial effects on human health.

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“…In the diversity analysis of olive trees, microsatellites, also called simple sequence repeats (SSRs), are the molecular markers of choice because of transfer ability, high polymorphic, co-dominance, and their relatively simple interpretation (Belaj et al, 2003 ). This type of molecular marker has been successfully used for olive tree variety identification, genetic diversity studies, and to evaluate the relationships between different varieties (Sarri et al, 2006 ; Bracci et al, 2009 ; Ercisli et al, 2011 ; di Rienzo et al, 2018 ; Lazović et al, 2018 ; El Bakkali et al, 2019 ; Dervishi et al, 2021 ). Due to the wide use of SSRs in olive trees, Baldoni et al ( 2009 ) published a set of recommended microsatellite markers and protocols for olive genotyping, along with a list of reference varieties suitable for the establishment of a worldwide database of genotypes.…”

Section: Introductionmentioning

confidence: 99%

Accessing Ancestral Origin and Diversity Evolution by Net Divergence of an Ongoing Domestication Mediterranean Olive Tree Variety

et al. 2021

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Olea europaea ‘Galega vulgar’ variety is a blend of West and Central Mediterranean germplasm with cultivated-wild admixture characteristics. ‘Galega vulgar’ is known for its high rusticity and superior-quality olive oil, being the main Portuguese variety with high impact for bioeconomy. Nevertheless, it has been replaced by higher-yielding and more adapted to intensive production foreign varieties. To clarify the potential ancestral origin, genetic diversity evolution, and existing genetic relationships within the national heritage of ‘Galega vulgar’, 595 trees, belonging to ancient and centenary age groups and prospected among ten traditional production regions, were characterized using 14 SSR markers after variety validation by endocarp measurements. Ninety-five distinguishable genets were identified, revealing the presence of a reasonable amount of intra-genetic and morphological variability. A minimum spanning tree, depicting the complete genealogy of all identified genets, represented the ‘Galega vulgar’ intra-varietal diversity, with 94% of the trees showing only a two-allele difference from the most frequent genet (C001). Strong correlations between the number of differentiating alleles from C001, the clonal size, and their net divergence suggested an ancestral monoclonal origin of the ‘Galega vulgar’, with the most frequent genet identified as the most likely origin of all the genets and phenotypic diversification occurring through somatic mutations. Genetic erosion was detected through the loss of some allele combinations across time. This work highlights the need to recover the lost diversity in this traditional olive variety by including ancient private genets (associated with potential adaptation traits) in future breeding programs and investing in the protection of these valuable resources in situ by safeguarding the defined region of origin and dispersion of ‘Galega vulgar’. Furthermore, this approach proved useful on a highly diverse olive variety and thus applicable to other diverse varieties due either to their intermediate nature between different gene pools or to the presence of a mixture of cultivated and wild traits (as is the case of ‘Galega vulgar’).

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Intra-varietal variability and genetic relationships among the homonymic East Adriatic olive ( Olea europaea L.) varieties

Cited by 15 publications

References 52 publications

Morphological and microsatellite analysis of the ancient Montenegrin olive variety ‘Žutica’ revealed different clones

Morphological and microsatellite analysis of the ancient Montenegrin olive variety ‘Žutica’ revealed different clones

Oleocanthal Quantification Using 1H NMR Spectroscopy and Polyphenols HPLC Analysis of Olive Oil from the Bianchera/Belica Cultivar

Accessing Ancestral Origin and Diversity Evolution by Net Divergence of an Ongoing Domestication Mediterranean Olive Tree Variety

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