Intra-specific genetic diversity and chemical profiling of different accessions of Clitoria ternatea L.

Ali, Zahid; Ganie, Showkat Hussain; Narula, Alka; Sharma, Manju; Srivastava, Prem S.

doi:10.1016/j.indcrop.2012.07.070

Cited by 21 publications

(14 citation statements)

References 17 publications

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“…Phytochemical and molecular markers have been simultaneously used to characterize the level of variation in several medicinal plant species, such as Withania spp., Ocimum basilicum , Zataria multiflora , Clitoria ternatea , Salvia sclarea , Ocimum selloi , Ocotea spp., and Satureja rechingeri . In the present study, ISSR markers were used for the first time to reveal the extent and distribution of the genetic diversity of twelve P .…”

Section: Introductionmentioning

confidence: 99%

“…[41] According to the literature information, ISSR markers have been efficiently used for the genetic analysis of several medicinal plant species. [42 -44] Phytochemical and molecular markers have been simultaneously used to characterize the level of variation in several medicinal plant species, such as Withania spp., [45] Ocimum basilicum, [46] Zataria multiflora, [47] Clitoria ternatea, [48] Salvia sclarea, [35] Ocimum selloi, [32] Ocotea spp., [49] and Satureja rechingeri. [37] [50] In the present study, ISSR markers were used for the first time to reveal the extent and distribution of the genetic diversity of twelve P. abrotanoides populations (PAbPs) from Iran, as a first step towards gaining a better knowledge of the genome diversity of the plant.…”

Section: Introductionmentioning

confidence: 99%

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Genetic and Chemical Diversity in Perovskia abrotanoides Kar. (Lamiaceae) Populations Based on ISSRs Markers and Essential Oils Profile

Pourhosseini

Hadian

Sonboli³

et al. 2018

Chemistry & Biodiversity

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Genetic and the essential oil composition variability among twelve Perovskia abrotanoides populations (PAbPs) growing wild in Iran were assessed by ISSR markers, GC-FID and GC/MS, respectively. Nine selected ISSR primers produced 119 discernible bands, of them 96 (80.7%) being polymorphic. Genetic similarity values among populations ranged between 0.07 and 0.79 which indicated a high level of genetic variation. Polymorphic information content, resolving power and marker index generated by ISSR primers were, 0.31, 6.14, and 3.32, respectively. UPGMA grouped PAbPs into four main clusters. Altogether, 38 chemical compounds were identified in the oils, and a relatively high variation in their contents was found. Camphor (11.9 - 27.5%), 1,8-cineole (11.3 - 21.3%), α-bisabolol (0.0 - 13.1%), α-pinene (5.9 - 10.8%), and δ-3-carene (0.1 - 10.5%) were the major compounds. Oxygenated monoterpenes (32.1 - 35.8%) and monoterpene hydrocarbons (25.7 - 30.4%) were the main groups of compounds in the oils studied. Cluster analysis and principal-component analysis were used to characterize the samples according to oil components. Four main chemotypes were found to be Chemotype I (camphor/1,8-cineol), Chemotype II (1,8-cineole/camphor), Chemotype III (camphor/1,8-cineol/α-bisabolol), and Chemotype IV (camphor/δ-3-carene/α-bisabolol). The information, provided here on P. abrotanoides populations, will be useful to introduce this plant into agricultural systems.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic and Chemical Diversity in Perovskia abrotanoides Kar. (Lamiaceae) Populations Based on ISSRs Markers and Essential Oils Profile

Pourhosseini

Hadian

Sonboli³

et al. 2018

Chemistry & Biodiversity

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“…Similar finding have been reported for Prunus cerasifera (Ayanoglu et al 2007 ) and Punica granatum (Jbir et al 2008 ). However, Ali et al ( 2013 ) reported well-structured clustering in Clitoria ternatea and Belaj et al ( 2010 ) for Andalusian and most Catalonian olive cultivars. Other than Rajasthan genotypes that remained scattered all over the dendrogram, all other genotypes formed perfect clusters by remaining confined within their respective clades.…”

Section: Resultsmentioning

confidence: 98%

Genetic diversity and chemical profiling of different populations of Convolvulus pluricaulis (convolvulaceae): an important herb of ayurvedic medicine

et al. 2014

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Convolvulus pluricaulis Choisy, commonly known as “Shankhpushpi”, is an ayurvedic medicinal plant recommended as a brain tonic to promote intellect and memory, eliminate nervous disorders and to treat hypertension. Because of increasing demand of the drug, this plant species has been over-exploited. As a consequence, many unrelated plants are being sold by the crude drug dealers in India in the name of “Shankhpushpi”. Information on its existing gene pool is currently lacking. We developed molecular (Random Amplification of Polymorphic DNA) and chemical (high performance liquid chromatography) markers that could distinguish the genuine plant species from its adulterants. Molecular characterization confirmed higher genetic variation at inter-zonal level as compared to intra-zonal populations. A total of 37 reproducible amplicons were generated of which 22 were polymorphic. The number of amplicons was in the range of 6–11 and genetic distance for the studied primers ranged from 0.07 to 0.34. Fifty nine per cent polymorphism was obtained across different geographical locations. Dendrogram studied through unweighted pair group method of arithmetic analysis differentiated all the genotypes into two major clusters, Cluster I had the single population of Rajasthan and Cluster II was represented by genotypes of Delhi, Haryana, Madhya Pradesh and Rajasthan. The Kaempferol content ranged from 0.07 to 0.49 mg/g and Delhi population was the highest accumulator.

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“…Similarly, two species of the parasite Cuscuta (C. reflexa and C. chinensis) have been distinguished with primers OPC-1, OPC-02, OPC-03, OPC-04, OPC-05, OPC-06, OPC-07 and OPC-08 (Khan et al 2010a). Ali et al (2013), while characterizing Clitoria ternatea at inter-zonal level identified complete monomorphism with primer OPN-02, therefore, the identification of this herb with OPN-02 is the good choice. Molecular characterization of Convolvulus pluricaulis revealed that primer OPN-09 is the specie specific primer for the herb (Ganie et al, 2015).…”

Section: Random Amplification Of Polymorphic Dna (Rapd)mentioning

confidence: 99%

Authentication of medicinal plants by DNA markers

et al. 2015

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Medicinal plants have been used worldwide for centuries to maintain health and to treat diseases, more so chronic diseases. However, adulteration and use of spurious materials as substitutes have become a major concern for users and industry for reasons of safety and efficacy. Therefore, authentication of medicinal plants is of utmost importance. Morphological, anatomical, chemical and DNA markers solve the problem by differentiating the genuine material from the adulterants, substitutes and spurious drugs. DNA markers use nucleotide sequences to identify species; it takes preference over the other two markers being not age dependent, tissue specific and having a higher discriminating power. Therefore, characterization of plants with such markers is an ideal approach for identification of medicinal plant species and populations/varieties of the same species. Availability of certified taxonomic specimens in herbaria is certainly required for unambiguous confirmation through final visual comparison and analysis.

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Intra-specific genetic diversity and chemical profiling of different accessions of Clitoria ternatea L.

Cited by 21 publications

References 17 publications

Genetic and Chemical Diversity in Perovskia abrotanoides Kar. (Lamiaceae) Populations Based on ISSRs Markers and Essential Oils Profile

Genetic and Chemical Diversity in Perovskia abrotanoides Kar. (Lamiaceae) Populations Based on ISSRs Markers and Essential Oils Profile

Genetic diversity and chemical profiling of different populations of Convolvulus pluricaulis (convolvulaceae): an important herb of ayurvedic medicine

Authentication of medicinal plants by DNA markers

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