Intra-puparial development of the Cochliomyia macellaria and Lucilia cuprina (Diptera, Calliphoridae)

Barros-Cordeiro, Karine Brenda; Pujol-Luz, José Roberto; Name, Khesller Patrícia Olázia; Báo, Sônia Nair

doi:10.1016/j.rbe.2016.06.009

Cited by 14 publications

(6 citation statements)

References 23 publications

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“…These studies frequently resulted in a series of images of the intra-puparial specimens, imaged from one or more orientation (most often dorsally), to show the morphological and colour changes that accompany metamorphosis. Examples of these, with varying degrees of detail and time resolution but all focussing entirely on external morphology of the intra-puparial specimens, have provided data for: Calliphora vicina [36], Lucilia sericata [37,38], Lucilia cuprina [39], Lucilia illustris [40], Chrysomya albiceps [41,42], Chrysomya megacephala [43], Chrysomya putoria [44] and Cochliomyia macellaria [39]. Similar studies have been undertaken on the forensically relevant scuttle flies, Megaselia spiracularis and Megaselia scalaris (Diptera: Phoridae) [45,46], and on the soldier fly, Hermetia illuscens (Diptera: Stratiomyidae) [47,48].…”

Section: Techniques (A) Light Microscopymentioning

confidence: 99%

Visualization of insect metamorphosis

Hall

Martín‐Vega

2019

Phil. Trans. R. Soc. B

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Metamorphosis and, in particular, holometaboly, the development of organisms through a series of discrete stages (egg, larva, pupa, adult) that hardly resemble one another but are finely adapted to specific roles in the life cycle of the organism, has fascinated and mystified humans throughout history. However, it can be difficult to visualize the dramatic changes that occur during holometaboly without destructive sampling, traditionally through histology. However, advances in imaging technologies developed mainly for medical sciences have been applied to studies of insect metamorphosis over the past couple of decades. These include micro-computed tomography, magnetic resonance imaging and optical coherence tomography. A major advantage of these techniques is that they are rapid and non-destructive, enabling virtual dissection of an organism in any plane by anyone who has access to the image files and the necessary software. They can also be applied in some cases to visualize metamorphosis in vivo , including the periods of most rapid and dramatic morphological change. This review focusses on visualizing the intra-puparial holometabolous metamorphosis of cyclorraphous flies (Diptera), including the primary model organism for all genetic investigations, Drosophila melanogaster , and the blow flies of medical, veterinary and forensic importance, but also discusses similar studies on other insect orders. This article is part of the theme issue ‘The evolution of complete metamorphosis’.

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Section: Techniques (A) Light Microscopymentioning

confidence: 99%

Visualization of insect metamorphosis

Hall

Martín‐Vega

2019

Phil. Trans. R. Soc. B

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“…Among the various cyclorrhaphans that have been studied, the developmental phases and main morphological markers are the same regardless of family (Cepeda-Palacios & Scholl, 2000;Jing, Zhang, Dou, Jiang, & Wang, 2019;Kanti-Sinha & Mahato, 2016;Pujol-Luz & Barros-Cordeiro, 2012). The pharate adult is always the longest phase, comprising between 50 and 90% of the entire pupal stage (Barros-Cordeiro, Pujol-Luz, Name, & Báo, 2016). Despite these broad generalities, there is a persistent controversy about the timing and nature of the two apolysis events (da Silva & Moura, 2019;Fraenkel & Bhaskaran, 1973).…”

Section: Discussionmentioning

confidence: 99%

Intra‐puparial development in the hoverflies Eristalinus aeneus and Eristalis tenax (Diptera: Syrphidae)

Campoy

Pérez‐Bañón

2020

Journal of Morphology

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The intra-puparial development of 150 pupae of Eristalinus aeneus (Scopoli, 1763) and Eristalis tenax (Linnaeus, 1758) was analyzed. Individuals were obtained from the sixth laboratory generation kept under artificial rearing conditions at the facilities of the University of Alicante (Spain). The experiment was conducted at 25 ± 1 C temperature, 50 ± 5% relative humidity, and 12:12 hr (L:D) of photoperiod. Groups of 10 pupae were collected every 6 hr over 48 hr, after that period, pupae were collected daily until the adult emergence. They were fixed in 5% formic acid and preserved in 70% ethanol. Fixed pupae were dissected and photographed. The chronology and morphological changes that take place during the intra-puparial development in both species were analyzed and compared. Five phases were observed: prepupa, before 6 hr; cryptocephalic pupa, between 6 and 24 hr; phanerocephalic pupa, between 24 and 30 hr; pharate adult, after 30 hr; and the adult imago, restricted to the very end of the development process just before adult emergence. In total, the intra-puparial development lasted 189 ± 4 hr in E. aeneus and 192 ± 3 hr in E. tenax, with the pharate adult the longest phase (some 81% of the total developmental time). These data can be used to develop accurate cold storage protocols during artificial rearing of both pollinator species, avoiding critical events during the development and increasing survival.

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“…Using insect morphological changes to estimate the occurrence time of an event is a popular method in forensic entomology. Intrapuparial chronology of many saprophagous flies, such as Lucilia sericata [21], Cochliomyia macellaria, and Lucilia cuprina [22] were studied and used in calculating the minimum postmortem intervals ( min PMI). The larval development is of equal importance in forensic cases [23].…”

Section: Discussionmentioning

confidence: 99%

First Insights into the Intrapuparial Development of Bactrocera dorsalis (Hendel): Application in Predicting Emergence Time for Tephritid Fly Control

Jing,

Zhang,

Dou

et al. 2019

Insects

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Intrapuparial development is a special pattern of metamorphosis in cyclorrhaphous flies, in which the pupa forms in an opaque, barrel-like puparium. This has been well studied in forensic insects for age estimations. In this study, the intrapuparial development of a quarantine agricultural pest, Bactrocera dorsalis (Hendel), was studied under a constant temperature of 27 ± 1 °C and 70 ± 5% relative humidity. Results showed that intrapuparial development could be divided into five stages: Larval-pupal apolysis, cryptocephalic pupa, phanerocephalic pupa, pharate adult, and emergent adult. It lays a morphology-based foundation for molecular mechanism studies and enhances the understanding of the physiological basis for changes in intrapuparial development. More importantly, the chronology of intrapuparial development can be used to predict the emergence time of tephritid flies, indicating when to spray insecticides to control these phytophagous agricultural pests. This may be an effective approach to reduce the use of insecticides and slow down the evolution of insecticidal resistance.

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Intra-puparial development of the Cochliomyia macellaria and Lucilia cuprina (Diptera, Calliphoridae)

Cited by 14 publications

References 23 publications

Visualization of insect metamorphosis

Visualization of insect metamorphosis

Intra‐puparial development in the hoverflies Eristalinus aeneus and Eristalis tenax (Diptera: Syrphidae)

First Insights into the Intrapuparial Development of Bactrocera dorsalis (Hendel): Application in Predicting Emergence Time for Tephritid Fly Control

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