Intra-individual variation of miRNA expression levels in human plasma samples

Wu, Jie; Cai, Hui; Xiang, Yong‐Bing; Matthews, Charles E.; Ye, Fei; Wang, Zheng; Cai, Qiuyin

doi:10.1080/1354750x.2018.1427794

Cited by 12 publications

(10 citation statements)

References 49 publications

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“…However, we studied knee OA in women only, and the number of participants is limited. It has been demonstrated that gender may influence the level of the expression of miRNAs [52]. Consequently, our results need to be confirmed by additional larger studies and particularly in males.…”

Section: Discussionmentioning

confidence: 65%

Association of circulating microRNAs with prevalent and incident knee osteoarthritis in women: the OFELY study

et al. 2020

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ObjectivesIn the context of the scarcity of biomarkers for knee osteoarthritis (OA), we examined the associations of prevalent and incident OA with the expression levels of serum miRNAs in subjects with and without OA.MethodsWith a next-generation sequencing approach, we compared the miRome expression of 10 women with knee OA and 10 age-matched healthy subjects. By real-time qPCR, we analyzed the expression levels of 19 miRNAs at baseline selecting 43 women with prevalent knee OA (Kellgren Lawrence score of 2/3), 23 women with incident knee OA over a 4-year follow-up and 67 healthy subjects without prevalent or incident OA matched for age and body mass index.ResultsSerum miR-146a-5p was significantly increased in the group of prevalent knee OA compared with controls (relative quantification (RQ); median [Interquartile range] 1.12 [0.73; 1.46] vs 0.85 [0.62; 1.03], p = 0.015). The likelihood of prevalent knee OA was significantly increased (odds ratio [95% confidence interval (CI)] 1.83 [1.21–2.77], p = 0.004) for each quartile increase in serum miR-146a-5p. The women with miR-146a-5p levels above the median (0.851) had a higher risk of prevalent knee OA compared to those below the median [95% CI] 4.62 [1.85–11.5], p = 0.001. Moreover, we found a significant association between the baseline level of serum miR-186-5p and the risk of incident knee OA (Q4 vs Q1–3; odds ratio [95% CI] 6.13 [1.14–32.9], p = 0.034).ConclusionWe showed for the first time that miR-146a-5p and miR-186-5p are significantly associated with prevalent and incident knee OA, respectively.

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Section: Discussionmentioning

confidence: 65%

Association of circulating microRNAs with prevalent and incident knee osteoarthritis in women: the OFELY study

et al. 2020

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“…Recent advances within the field of sequencing (SLAM-seq [ 69 ]) using tissue-specific incorporation of 4-thiouridine (4-TU) could prove useful for in vivo tracking of miRNAs released into circulation. Despite miRNA levels demonstrating high correlations between repeated samples over a larger sampling period in healthy individuals [ 70 , 71 ], more longitudinal studies within pregnant women are needed should miRNAs be utilized in clinical practice.…”

Section: Discussionmentioning

confidence: 99%

The Temporal Profile of Circulating miRNAs during Gestation in Overweight and Obese Women with or without Gestational Diabetes Mellitus

et al. 2022

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Circulating non-coding microRNAs (miRNAs) are important for placentation, but their expression profiles across gestation in pregnancies, which are complicated by gestational diabetes mellitus (GDM), have not been fully established. Investigating a single time point is insufficient, as pregnancy is dynamic, involving several processes, including placenta development, trophoblast proliferation and differentiation and oxygen sensing. Thus, the aim of this study was to compare the temporal expression of serum miRNAs in pregnant women with and without GDM. This is a nested case-control study of longitudinal data obtained from a multicentric European study (the ‘DALI’ study). All women (n = 82) were overweight/obese (BMI ≥ 29 kg/m2) and were normal glucose tolerant (NGT) at baseline (before 20 weeks of gestation). We selected women (n = 41) who were diagnosed with GDM at 24–28 weeks, according to the IADPSG/WHO2013 criteria. They were matched with 41 women who remained NGT in their pregnancy. miRNA (miR-16-5p, -29a-3p, -103-3p, -134-5p, -122-5p, -223-3p, -330-3p and miR-433-3p) were selected based on their suggested importance for placentation, and measurements were performed at baseline and at 24–28 and 35–37 weeks of gestation. Women with GDM presented with overall miRNA levels above those observed for women remaining NGT. In both groups, levels of miR-29a-3p and miR-134-5p increased consistently with progressing gestation. The change over time only differed for miR-29a-3p when comparing women with GDM with those remaining NGT (p = 0.044). Our findings indicate that among overweight/obese women who later develop GDM, miRNA levels are already elevated early in pregnancy and remain above those of women who remain NGT during their pregnancy. Maternal circulating miRNAs may provide further insight into placentation and the cross talk between the maternal and fetal compartments.

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“…The levels of 75 miRs revealed an intra-class coefficient (ICC) of > 0.5 when analysed in a subject at two time points 6 to 12 months apart, suggesting reasonable similarity, with a median ICC of 0.4 for the total 185 miRs. Notably, ICC was higher for miRs with higher plasma levels or higher detection rates (Wu et al 2018b).…”

Section: Inter-and Intra-individual Variability In Basal Mir Expressionmentioning

confidence: 96%

“…For miR measurements to be reliable indicators of injury, a good understanding of the presence and significance of variation in their basal levels is required. Intra-individual variation has been assessed by Wu et al (2018b), where circulating miR levels in repeated samples were collected from fifty-one healthy volunteers over a 6-12-month period. 185 miRs were detected in at least 10% of plasma samples, 69 in 50% and 28 in 90%.…”

Section: Inter-and Intra-individual Variability In Basal Mir Expressionmentioning

confidence: 99%

Systems analysis of miRNA biomarkers to inform drug safety

Schofield

Brown

et al. 2021

Arch Toxicol

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AbstractmicroRNAs (miRNAs or miRs) are short non-coding RNA molecules which have been shown to be dysregulated and released into the extracellular milieu as a result of many drug and non-drug-induced pathologies in different organ systems. Consequently, circulating miRs have been proposed as useful biomarkers of many disease states, including drug-induced tissue injury. miRs have shown potential to support or even replace the existing traditional biomarkers of drug-induced toxicity in terms of sensitivity and specificity, and there is some evidence for their improved diagnostic and prognostic value. However, several pre-analytical and analytical challenges, mainly associated with assay standardization, require solutions before circulating miRs can be successfully translated into the clinic. This review will consider the value and potential for the use of circulating miRs in drug-safety assessment and describe a systems approach to the analysis of the miRNAome in the discovery setting, as well as highlighting standardization issues that at this stage prevent their clinical use as biomarkers. Highlighting these challenges will hopefully drive future research into finding appropriate solutions, and eventually circulating miRs may be translated to the clinic where their undoubted biomarker potential can be used to benefit patients in rapid, easy to use, point-of-care test systems.

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Intra-individual variation of miRNA expression levels in human plasma samples

Cited by 12 publications

References 49 publications

Association of circulating microRNAs with prevalent and incident knee osteoarthritis in women: the OFELY study

Association of circulating microRNAs with prevalent and incident knee osteoarthritis in women: the OFELY study

The Temporal Profile of Circulating miRNAs during Gestation in Overweight and Obese Women with or without Gestational Diabetes Mellitus

Systems analysis of miRNA biomarkers to inform drug safety

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