Intra-individual variability of ITS regions in entomopathogenic nematodes (Steinernematidae: Nematoda): implications for their taxonomy

Půža, Vladimı́r; Chundelová, Daniela; Nermuť, Jiří; Žurovcová, Martina; Mráček, Zdenĕk

doi:10.1007/s10526-015-9664-5

Cited by 25 publications

(18 citation statements)

References 18 publications

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“…Considering the high morphometrical variability of S. feltiae, molecular data are fairly useful (36); however, a marker such as the ITS region, which has proven useful in resolving phylogenetic relationships in EPN, has exhibited intraspeci c and intraindividual variability for this species as well as others in the feltiae and glaseri group, (42). According to these authors, considering the frequency of intraindividual variability, sequencing of the D2-D3 region of 28S appears to be necessary to con rm species status.…”

Section: Discussionmentioning

confidence: 99%

Morphological, Molecular and Ecological Characterization of a Native Isolate of Steinernema Feltiae (Rhabditida: Steinernematidae) From Southern Chile

Flores

Alvarado

Lankin

et al. 2020

Preprint

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Background: Steinernema feltiae is an entomopathogenic nematode used in biological control programs with a global distribution. It has been shown that populations of S. feltiae may have phenotypic plasticity derived from local adaptation and may vary in different traits, such as location and penetration of the host. This is the first time that a Chilean isolate has been described in detail, taking into account morphological, molecular and ecological characteristics. Methods: S. fealtiae was detected a few years ago in southern regions of Chile in the town Lican Ray and was morphologically and morphometrically described, along with a molecular description based on the ITS and D2-D3 regions. Some ecological characteristics were determined, including the temperature requirements for completion of its life cycle and the effect of three water levels in soil for optimal reproduction. Results: Morphometric characteristics of different life stages show large intraspecific variability in averages and ranks compared with isolates from different geographical origins. The molecular data also show intraspecific variability with respect to other isolates. The lower, optimal and higher temperatures found to limit the infestation and reproduction of Galleria mellonella were 10, 20 and 30 °C respectively, and emergence from the host larvae occurred approximately ten days after inoculation. Differences were observed in offspring emerging from the host, and the 120 infective juveniles (IJ)/larvae dose was the most prolific at 20 °C. The water content of the soil did not affect the number of infective juvenile invaders, penetration efficacy (%), and time of emergence of the IJ or offspring per larvae, but it caused a delay in achieving full mortality at the permanent wilting point with respect to saturation and field capacity.Conclusions: Data obtained in this study are valuable since they outline some environmental requirements of this strain to perform optimally in the event of being used as a soil pest bioantagonist.

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Section: Discussionmentioning

confidence: 99%

Morphological, Molecular and Ecological Characterization of a Native Isolate of Steinernema Feltiae (Rhabditida: Steinernematidae) From Southern Chile

Flores

Alvarado

Lankin

et al. 2020

Preprint

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“…In addition, the host immune response could be better understood with transcriptomic analyses following nematodes infection [ 186 , 187 , 188 ]. Genetic analyses, including sequencing, are particularly useful to better understand the diversity of nematodes [ 189 , 190 ] and their symbiotic bacteria [ 191 , 192 ], and to study their phylogenetic relationships [ 193 ]. Such phylogenies can help understand the evolution of certain traits.…”

Section: Discussionmentioning

confidence: 99%

Transmission Success of Entomopathogenic Nematodes Used in Pest Control

Labaude

Griffin

2018

Insects

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Entomopathogenic nematodes from the two genera Steinernema and Heterorhabditis are widely used as biological agents against various insect pests and represent a promising alternative to replace pesticides. Efficacy and biocontrol success can be enhanced through improved understanding of their biology and ecology. Many endogenous and environmental factors influence the survival of nematodes following application, as well as their transmission success to the target species. The aim of this paper is to give an overview of the major topics currently considered to affect transmission success of these biological control agents, including interactions with insects, plants and other members of the soil biota including conspecifics.

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“…al. , 2015). Unfortunately, there is no universal threshold sequence identity of the ITS region for species estimation among EPN.…”

Section: Discussionmentioning

confidence: 99%

Characterization of PolishSteinernema silvaticumisolates (Nematoda: Steinernematidae) using morphological and molecular data

et al. 2018

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Four strains of entomopathogenic nematodes were isolated with a live trap method in southern Poland. The isolates were identified as Steinernema silvaticum based on morphological, morphometric and molecular data. Infective juveniles of Polish S. silvaticum isolates differ in body length from S. weiseri (951 vs 740 μm, respectively), and in the hyaline tail portion from S. kraussei (48 vs 38%, respectively). First-generation males of S. silvaticum are longer than those of S. kraussei, S. weiseri and S. ichnusae (1829 vs 1400, 1180 and 1341 μm, respectively). Males of S. silvaticum and a sister species S. kraussei can be distinguished by the distance from the anterior end to the nerve ring (142 vs 105 μm), spicule (66 vs 49 μm) and gubernaculum length (45 vs 33 μm), and the presence of a mucron. The analysis of internal transcribed spacer (ITS), D2-D3 and cox1 sequences of the tested nematodes revealed differences of 3-5%, 3% and 12-13%, respectively, from S. kraussei strains. The phylogeny of both nuclear and mitochondrial genes indicated close relationships of the Polish S. silvaticum isolates with S. kraussei, S. oregonense and S. cholashanense. The reproductive isolation of the studied isolates was confirmed by hybridization tests with other European feltiae-kraussei group representatives. This study has supplemented the original description of S. silvaticum with morphological and morphometric characterization of the first-generation males and females. This is also the first molecular study of this species based on a multi-gene approach.

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Intra-individual variability of ITS regions in entomopathogenic nematodes (Steinernematidae: Nematoda): implications for their taxonomy

Cited by 25 publications

References 18 publications

Morphological, Molecular and Ecological Characterization of a Native Isolate of Steinernema Feltiae (Rhabditida: Steinernematidae) From Southern Chile

Morphological, Molecular and Ecological Characterization of a Native Isolate of Steinernema Feltiae (Rhabditida: Steinernematidae) From Southern Chile

Transmission Success of Entomopathogenic Nematodes Used in Pest Control

Characterization of PolishSteinernema silvaticumisolates (Nematoda: Steinernematidae) using morphological and molecular data

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