Intra-bladder wall transplantation of bone marrow mesenchymal stem cells improved urinary bladder dysfunction following spinal cord injury

Salehi‐Pourmehr, Hanieh; Rahbarghazi‬, Reza; Mahmoudi, Javad; Roshangar, Leila; Chapple, Christopher R.; Hajebrahimi, Sakineh; Abolhasanpour, Nasrin; Azghani, Mahmood Reza

doi:10.1016/j.lfs.2019.02.011

Cited by 18 publications

(11 citation statements)

References 69 publications

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“…Stem cell transplantation has been investigated in tissue regeneration for years; however, it still has some limitations [ 33 , 34 ]. Over the past few years, exosomes, a novel type of intercellular communication device, have been employed as a good biological carrier for local or systemic small RNA delivery for treating stroke or spinal cord injury and other central nervous system diseases.…”

Section: Discussionmentioning

confidence: 99%

Rat Bone Mesenchymal Stem Cell‐Derived Exosomes Loaded with miR‐494 Promoting Neurofilament Regeneration and Behavioral Function Recovery after Spinal Cord Injury

Huang

Lin

Yang

et al. 2021

Oxidative Medicine and Cellular Longevity

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Exosomes (Exo) exhibit numerous advantages (e.g., good encapsulation, high targeting efficiency, and easy to penetrate the blood-brain barrier to the central nervous system). Exosomes are recognized as prominent carriers of mRNAs, siRNAs, miRNAs, proteins, and other bioactive molecules. As confirmed by existing studies, miR-494 is important to regulate the occurrence, progression, and repair of spinal cord injury (SCI). We constructed miR-494-modified exosomes (Exo-miR-494). As indicated from related research in vitro and vivo, Exo-miR-494 is capable of effectively inhibiting the inflammatory response and neuronal apoptosis in the injured area, as well as upregulating various anti-inflammatory factors and miR-494 to protect neurons. Moreover, it can promote the regeneration of the neurofilament and improve the recovery of behavioral function of SCI rats.

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Section: Discussionmentioning

confidence: 99%

Rat Bone Mesenchymal Stem Cell‐Derived Exosomes Loaded with miR‐494 Promoting Neurofilament Regeneration and Behavioral Function Recovery after Spinal Cord Injury

Huang

Lin

Yang

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…Despite numerous MSC-based therapies have been reported to improve function outcome of underactive bladder in rat models [ 36 – 38 ], a major limitation to the use of MSCs in clinical applications is their poor viability at the site of injury due to the hyperglycemia-induced ischemic microenvironment and to anoikis driven by the loss of cell adhesion. Therefore, to improve the long-term survival of the transplanted MSCs, strategies to regulate apoptotic signaling and enhance cell proliferation and adhesion have been developed, such as genetic modifications [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effect of integrin-linked kinase gene-modified bone marrow-derived mesenchymal stem cells for streptozotocin-induced diabetic cystopathy in a rat model

et al. 2020

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Background: Diabetic cystopathy (DCP) is a chronic complication of diabetes mainly within the submucosal and muscular layers of the bladder due to the hyperglycemia-induced ischemia. As no effective therapies are currently available, the administration of optimized mesenchymal stem cells (MSCs) provides a potential treatment of DCP. Thus far, new strategy, such as genetic modification of MSCs, has been developed and has shown promising outcomes of various disorders. Methods: This study was conducted using integrin-linked kinase (ILK) gene-modified bone marrow-derived stem cells (BMSCs) for streptozotocin (STZ)-induced diabetic cystopathy in a rat model. In total, 68 male Sprague-Dawley rats were randomized into five groups: sham control (control group, n = 10); DCP model alone (DM group, n = 10); DCP rats intravenously treated with BMSCs (BMSC group, n = 16); DCP rats accepted adenoviral vector-infected BMSCs (Ad-null-BMSC group, n = 16) and DCP rats accepted ILK adenoviral vector-infected BMSCs (Ad-ILK-BMSC group, n = 16). Diabetic rats accepted cell transplantation in the experimental group (2 rats per group) were sacrificed for the bladder tissue on the third day, 7th day, and 14th day of treatment respectively ahead of schedule. At 4 weeks after treatment, all rats in five groups accepted urodynamic studies to evaluate bladder function and were sacrificed for bladder tissue. Results: Our data showed that the underactive bladder function was significantly improved in DCP rats intravenously treated with ILK gene-modified BMSCs compared to those in the DM, BMSCs, and Ad-null-BMSC group. Meanwhile, we found that gene-modified BMSC treatment significantly promoted the activation of the AKT/ GSK-3β pathway by increasing phosphorylation and led to the enhancement of survival. In addition, the expression levels of angiogenesis-related protein vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and stromal cell-derived factor-1 (SDF-1) were significantly higher in the Ad-ILK-BMSC group than that in the DM, BMSCs, and Ad-null-BMSC group as assessed by enzyme-linked immunosorbent assay and western blot. As two indicators of vascular endothelial cell markers, the expression of von Willebrand factor (vWF) and CD31 by

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“…Using 6‐0 non‐absorbable with monofilament suture, a purse‐string suture was carried out on the bladder dome under a surgical microscope. The collar catheter tip was implanted at the dome of the bladder after a small incision, then the suture was tightened around the collar part of the catheter with a surgeon's knot, according to our previous study . After suturing, the bladder was filled with saline to the leak point to ensure that no leakage occurred at the implantation site, as at this moment, leakage was observed in the urethral meatus.…”

Section: Methodsmentioning

confidence: 99%

Effect of cerebrolysin on bladder function after spinal cord injury in female Wistar rats

et al. 2019

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Abbreviations & Acronyms ANOVA = analysis of variance BBB = Basso-Beattie-Bresnahan BDNF = brain-derived neurotrophic factor CL = cerebrolysin CNS = central nervous system DSD = detrusor sphincter dyssynergia GABA = gammaaminobutyric acid GDNF = glial cell linederived neurotrophic factor IGF-I = insulin-like growth factor i.p. = intraperitoneal MVP = maximum voiding pressure NDO = neurogenic detrusor overactivity NGB = neurogenic bladder NGF = nerve growth factor NT = neurotrophin SCI = spinal cord injury Correspondence: SakinehObjectives: To study the effect of cerebrolysin on bladder function after spinal cord injury using functional measurements in rats. Methods: A total of 60 female rats were enrolled in this study. After induction of complete transection at T9-T10 spinal vertebrae, cerebrolysin was injected intraperitoneally, and daily in three dosages until 7 days (1 week) and continued until 28 days (4 weeks) in three groups to show the impact of that on the bladder function. Urodynamic parameters were measured in the different groups. Results: Cerebrolysin injection in a dose of 1 mL/kg for 1 week showed a slight improvement in urodynamic parameters. However, infusion of 2.5 and 5 mL/kg cerebrolysin for 1 week caused an elevation in contractions and a decrease in compliance. In long-term 2.5 mL/kg cerebrolysin injection, an improvement in compliance was observed, despite relative contractions. Furthermore, the bladder pressure pattern in the 2.5 mL/kg infused rats for 4 weeks was similar to the control group, but in the group receiving 5 mL/kg cerebrolysin for 4 weeks, reduced bladder contractions and function were observed. Conclusions: Our findings suggest that cerebrolysin might be able to inhibit the emergence of neurogenic detrusor overactivity in spinal cord injured female rats.

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Intra-bladder wall transplantation of bone marrow mesenchymal stem cells improved urinary bladder dysfunction following spinal cord injury

Cited by 18 publications

References 69 publications

Rat Bone Mesenchymal Stem Cell‐Derived Exosomes Loaded with miR‐494 Promoting Neurofilament Regeneration and Behavioral Function Recovery after Spinal Cord Injury

Rat Bone Mesenchymal Stem Cell‐Derived Exosomes Loaded with miR‐494 Promoting Neurofilament Regeneration and Behavioral Function Recovery after Spinal Cord Injury

Therapeutic effect of integrin-linked kinase gene-modified bone marrow-derived mesenchymal stem cells for streptozotocin-induced diabetic cystopathy in a rat model

Effect of cerebrolysin on bladder function after spinal cord injury in female Wistar rats

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