Intra-articular transplantation of porcine adipose-derived stem cells for the treatment of canine osteoarthritis: A pilot study

Abstract

patient was then discharged to the owner and, at 2, 6, and 12 wk, followed up with orthopedic assessment, owner questionnaire, X-ray imaging, and force-plate gait analysis. RESULTS:Porcine ADSCs expressed mesenchymal stem cell markers CD90 and CD105. Injection of porcine ADSCs into canine stifle joints did not cause any inflammatory or allergic reactions. Orthopedic evaluation found improvements in two dogs, particularly at the longest time point. Owners' evaluation found increased capacity and decreased pain in all three dogs' activities such as walking and running. Radiographic evaluation did not find statistically significant differences before and after treatment. Force-plate analysis found significant improvements in all three dogs after treatment. CONCLUSION:Xenotransplantation of ADSCs for the treatment of OA is feasible. Further studies are needed to validate this novel treatment modality, which can then be implemented for the routine treatment of OA in veterinary medicine. METHODS:To enroll in this study dogs must have stifle joint OA that had lasted ≥ 3 mo and been treated with OA medication without significant improvement. Three dogs fulfilled these criteria and were thus subjects for ADSCs treatment. ADSCs were isolated from abdominal adipose tissue of a 2-mo-old female Yorkshire pig. Their stem cell marker expression was examined by immunofluorescence staining. For treatment, 5 million ADSCs were injected into the diseased joint of each dog. In the next 48 h, the patient was observed for signs of inflammatory and allergic reactions. The

Quality of Life of Elderly and Obese Animal Models with Severe Knee Osteoarthritis after a Single Injection of Mesenchymal Stem Cells

“…The ARomDe score monitors the quality of life via the measurement of three dimensions, including pain and function recovery and behavior. It is consistent with many previous studies, which generally evaluate the improvement of models and human with knee osteoarthritis via the achievement of pain relief [2,7,14,19,20,26,28,29] and function recovery [1,2,26,27] and emotional behavior [1,19,20,27].…”

“…MSC transplantation has been studied in various animal models and achieved the success, such as significantly improvement of joint function in equine [19], improving mobility of dog's OA joint [20], inducing reparation of knee cartilage [22]. Our work showed consistent results of improvement of OA joint and function recovery in animal models.…”

Effect of intraarticular inoculation of mesenchymal stem cells in dogs with hip osteoarthritis by means of objective force platform gait analysis: concordance with numeric subjective scoring scales

“…With a force platform, limb function is commonly analyzed measuring the peak vertical force (PVF, maximal force applied during stance phase) and vertical impulse (VI, total force applied over time) to quantitatively assess the degree of lameness [ 26 – 28 ].…”

Detection of Repair of the Zone of Calcified Cartilage with Osteoarthritis through Mesenchymal Stem Cells by Ultrashort Echo Time Magnetic Resonance Imaging

“…Issues include hardware and software limitations, software availability, and a lack of validation studies. [ 48 ] Further studies on UTE MRI are needed to determine how it can effectively be used to monitor the progress of treatment of the ZCC with OA using MSCs in vivo .…”