Intra-articular injection of mono-iodoacetate induces osteoarthritis of the hip in rats

Miyamoto, Shuichi; Nakamura, Junichi; Ohtori, Seiji; Orita, Sumihisa; Omae, Takanori; Nakajima, Takayuki; Suzuki, Takane; Takahashi, Kazuhisa

doi:10.1186/s12891-016-0985-z

Cited by 25 publications

(45 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…On the basis of methods previously published, all rats were anesthetized with an intraperitoneal injection of 0.3 mg/kg of medetomidine, 4.0 mg/kg of midazolam, and 5.0 mg/kg of butorphanol and treated aseptically throughout the experiments. Using a 27‐gauge needle, the following solutions were injected into the right hip joint of each rat using the posterior approach: 25 µl of sterile saline with 2 mg of MIA (Sigma‐Aldrich, St. Louis, MO) and 1% of retrograde neurotracer fluorogold (FG; Fluorochrome, Denver, CO) in the 12 rats, and 25 µl of sterile saline with 1% FG in the six rats (Sham group). The injected agent was confirmed to be restricted to the joint cavity.…”

Section: Methodsmentioning

confidence: 99%

Analgesic Effect of Duloxetine on an Animal Model of Monosodium Iodoacetate‐Induced Hip Osteoarthritis

Kawarai

Orita

Nakamura

et al. 2019

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

We investigated the efficacy of duloxetine on hyperalgesia, histopathological and radiographic findings, pain-related sensory innervation of dorsal-root ganglia (DRG), and spinal changes in a rat model of induced hip osteoarthritis (OA). The right hip joints of male Sprague-Dawley rats (n = 6 rats/group) in the Sham group were injected with 25 μl of sterile saline and 25 μl of sterile saline with 2 mg of monosodium iodoacetate (MIA) were injected to the MIA + Vehicle and MIA + Duloxetine groups. We injected duloxetine 20 mg/kg intraperitoneally in the MIA + Duloxetine group 28 days after injection, whereas rats in the MIA + Vehicle group were injected with 0.5 ml of 20% dimethyl sulfoxide. We assessed hyperalgesia, histopathological changes, immunoreactive (-ir) neurons for calcitonin generelated peptide and activating transcription factor 3 in DRG, and immunoreactive neurons for ionized-calcium-binding adaptor molecule 1 (Iba1) in the dorsal horn of the spinal cord. MIA administration into the hip joint let to mechanical hyperalgesia of the ipsilateral hind paw (p < 0.05). A single injection of duloxetine significantly attenuated it in induced hip OA (p < 0.05) and suppressed the number of Iba1-ir microglia of the ipsilateral dorsal horn (p < 0.05). These results suggest that a single injection of duloxetine suppressed mechanical hyperalgesia and may influence the expression of Iba1 in the microglia of the ipsilateral dorsal horn in the MIA-induced hip OA. This finding implies the inhibitory effects of duloxetine against neuropathic pain, which may lead to a change of microglial activities.

show abstract

Section: Methodsmentioning

confidence: 99%

Analgesic Effect of Duloxetine on an Animal Model of Monosodium Iodoacetate‐Induced Hip Osteoarthritis

Kawarai

Orita

Nakamura

et al. 2019

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…The protocols for the animal procedures in these experiments followed the 1996 revision of the National Institutes of Health guidelines for the Care and Use of Laboratory Animals and received approval from the ethics committee of our institution. Based on methods previously published elsewhere, all rats were anesthetized with an intraperitoneal injection of sodium pentobarbital (40 mg/kg) and treated aseptically throughout the experiments. Using a 27‐gauge needle, the following solutions were injected into the right hip joint of each rat using a posterior approach: 25 μl of sterile saline with 2 mg of MIA (Sigma–Aldrich, St. Louis, MO) and 1% of the retrograde neurotracer FG (Fluorochrome, Denver, CO) in MIA group rats ( n = 30), and 25 μl of sterile saline with 1% of the retrograde neurotracer FG in the sham group rats ( n = 30).…”

Section: Methodsmentioning

confidence: 99%

Pain-related behavior and the characteristics of dorsal-root ganglia in a rat model of hip osteoarthritis induced by mono-iodoacetate

et al. 2017

Self Cite

View full text Add to dashboard Cite

The principal aim of this study was to clarify the time course of pain-related behavior and pain-related sensory innervation in a rat model of hip osteoarthritis (OA) induced by intra-articular injection of mono-iodoacetate (MIA). Using 6-week-old male Sprague Dawley rats, 25 μl of sterile saline of 1% Fluoro-Gold solution (FG) (control group; n = 30) and 25 μl of sterile saline of 1% FG with 2 mg of MIA (MIA group; n = 30) was injected into the right hip joints. Gait function was evaluated using a CatWalk system after 7, 14, 28, 42, and 56 days (n = 5, respectively). Neurons in the dorsal root ganglion (DRG) between L1 and L5 were immunostained for calcitonin gene-related peptide (CGRP) and activating transcription factor-3 (ATF3). Gait analysis revealed the mean six parameters of hind paws at all time points were significantly lower in the MIA group (p = 0.05). The number of CGRP-immunoreactive (-IR) DRG neurons was significantly increased on days 7, 14, 28, and 42 peaking at 14 days in the MIA group. By contrast, expression of ATF3-IR in FG-labeled DRG neurons was significantly increased on days 42 and 57. The FG-labeled DRG neurons were distributed between L1 and L5, mainly at the L4 level. Pain-related behavior indicated by gait disturbance was observed in a MIA model of hip OA in rat. Early elevation of CGRP expression and late expression of ATF-3 were demonstrated in DRG neurons, possibly reflecting inflammatory pain and neuropathic pain in hip OA. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:1424-1430, 2017.

show abstract

“…Artrografik madde olarak Urografin (Bayer-Germany) seçildi. Denek hayvanlarının boyutu da düşünülerek artrografik madde dozu sırası ile toplamda 0.05 cc-0.1 cc-0.2 cc olacak şekilde kalça eklemine floroskopi kontrolünde verilmesi planlandı (5) . Kalçanın denek hayvanında çok küçük olması ve ekleme girildiğinden emin olunması için önce denek hayvanına anestezi uygulandı.…”

Section: Gereç Ve Yöntemunclassified

Injection of different amounts of arthrographic material to hip joint cause subluxation?: Experimental study

Yılmaz¹,

Şirin²,

Tümentemur³

et al. 2017

IKSST

View full text Add to dashboard Cite

ÖZAmaç: Kalça eklemlerine artrografik madde verilerek, femur başının asetabulum ile olan ilişkisi anlaşılabilmektedir. Ancak verilen artrografik madde miktarının kalça redüksiyonunu değiştirip değiştirmediğini bilinmemektedir. Amacımız denek hayvanları üzerinde kalça eklemine enjekte edilen artrografik maddenin değişen miktarının kalça ekleminde subluksasyon oluşturup oluşturmadığını deneysel olarak araştırmaktır. Gereç ve Yöntem:Yapılan deneysel çalışmada 6 sıçanın 12 kalçasına sırası ile 0.05 cc, 0.1 cc ve 0.2 cc. artrografik madde verildi. Verilen her miktar sonrası kalçanın redüksiyonunun değişimi radyolojik olarak görüntülendi ve femur başı tepe noktası ile asetabulum en derin noktası arasındaki mesafeler ölçülerek istatistiksel olarak değerlendirildi. Bulgular: Verilen artrografik maddenin 0.05, 0.1 ve 0.2 cc'lik miktarlarına bağlı olarak istatistiksel 10 olarak anlamlı bir farklılık gözlenmemiştir (p>0.05).Sonuç: Sonuç olarak, verilen artrografik madde miktarının kalça redüksiyonu etkilemediği sonucu ortaya çıksa da, eklem içine verilen artrografik madde miktarının kalça ekleminde femur başı ile pelvis arasındaki doğal ilişkiyi etkileyebileceği hâlen yalnızca bir teori olarak kalmaya devam etmektedir. ABSTRACT Objective:The relation between the femoral head and the acetabulum can be analysed by injecting arthtographic material into the hip joint. However it is not well determined whether the amount of the arthrographic material has any effect on hip reduction. We aimed to investigate experimentally whether the injection of different amounts of arthrographic material to hip joint can cause subluxation or not. Material and Methods:In this experimental study, 12 hips of 6 rats were injected 0.05 cc, 0.1 cc and 0.2 cc arthrographic material, respectively. Following each intraarticular injection of arthrographic material the resulting radiological change in hip reduction is visualised and the distance between tip of the femoral head and the deepest point of asetabulum was measured and evaluated statistically.Results: A statistically significant difference was not observed based on the the different amounts (0.05 cc, 0.1 cc and 0.2 cc) of the arthrographic material (p>0.05). Conclusion:So as a result, although it seems that the amount of arthrographic material does not have any effect on hip reduction,it still remains as a theory that the natural relationship between the femoral head and acetabulum can be influenced by the amount of the arthrographic contrast material injected into the hip joint.

show abstract

Intra-articular injection of mono-iodoacetate induces osteoarthritis of the hip in rats

Cited by 25 publications

References 23 publications

Analgesic Effect of Duloxetine on an Animal Model of Monosodium Iodoacetate‐Induced Hip Osteoarthritis

Analgesic Effect of Duloxetine on an Animal Model of Monosodium Iodoacetate‐Induced Hip Osteoarthritis

Pain-related behavior and the characteristics of dorsal-root ganglia in a rat model of hip osteoarthritis induced by mono-iodoacetate

Injection of different amounts of arthrographic material to hip joint cause subluxation?: Experimental study

Contact Info

Product

Resources

About