Intra-articular injection of flavopiridol-loaded microparticles for treatment of post-traumatic osteoarthritis

Sangsuwan, Rapeepat; Yik, Jasper H.N.; Owen, Matthew J.; Liu, Gang-yu; Haudenschild, Dominik R.; Lewis, Jamal S.

doi:10.1016/j.actbio.2022.06.042

Cited by 10 publications

(7 citation statements)

References 46 publications

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“…In the CIA animal model, free IR-780, PM-HA@IR-780 NPs, and PAM-HA@IR-780 NPs were administered via joint cavity injection separately, and the distribution of IR-780 was tracked by fluorescence imaging using an in vivo imaging system (IVIS). IVIS analysis demonstrated (Figures A and S10) that the clearance of free IR-780 was faster than that of PM-HA@IR-780 NPs and PAM-HA@IR-780 NPs in both healthy and CIA mouse joints, which may be attributed to the large size of the NPs-mediated slower lymphatic excretion and sustained release of IR-780 under ROS conditions . Furthermore, in the CIA group, slightly more accumulation of fluorescent molecules could be found in the joints of PAM-HA@IR-780 NPs-treated rats rather than PM-HA@IR-780 NPs-treated rats.…”

Section: Resultsmentioning

confidence: 97%

“…IVIS analysis demonstrated (Figures 5A and S10) that the clearance of free IR-780 was faster than that of PM-HA@ IR-780 NPs and PAM-HA@IR-780 NPs in both healthy and CIA mouse joints, which may be attributed to the large size of the NPs-mediated slower lymphatic excretion and sustained release of IR-780 under ROS conditions. 53 Furthermore, in the CIA group, slightly more accumulation of fluorescent molecules could be found in the joints of PAM-HA@IR-780 NPs-treated rats rather than PM-HA@IR-780 NPs-treated rats. It might be due to the increased surface area of the exposed bone matrix in the cartilage surface of the CIA group as a result of knee damage, and Ald can bind to the hydroxyapatite of the bone matrix, thereby facilitating the retention of PAM-HA@ IR-780 NPs in the knee joint.…”

Section: Study Of In Vitro Anti-inflammatory Effects and Mechanismsmentioning

confidence: 90%

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Drug in Therapeutic Polymer: Sinomenine-Loaded Oxidation-Responsive Polymeric Nanoparticles for Rheumatoid Arthritis Treatment

Shang,

Sun,

Zhang

et al. 2023

ACS Appl. Mater. Interfaces

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Rheumatoid arthritis (RA) is a chronic inflammatory joint disease that frequently involves cartilage damage and the destruction of the bone structure, ultimately resulting in disability and long-term pain. It is clear that overexpression of reactive oxygen species (ROS) and the complex inflammatory microenvironment are the main causes of RA pathogenesis; thereby, the efficacy of any single-drug treatment is limited. Herein, we formulated a therapeutic hyaluronic acid derivative (PAM-HA) with adsorption capacity to the subchondral bone, a long retention time within inflamed joints, and ROSscavenging capacity, which was used as a drug carrier for realizing the controlled release of sinomenine (Sin) within arthritic joints. This "drug in therapeutic polymer" design strategy was aimed at realizing antioxidant and anti-inflammatory combination therapy for RA. In vivo experiments suggest that PAM-HA@Sin NPs can be retained in the inflamed joints of rats for a long time compared with commercially available free Sin injections. As expected, therapeutic PAM-HA polymeric carriers can increase joint lubrication and reduce oxidative stress, while the released Sin induces downregulation of proinflammatory factors (TNF-α and IL-1β) and upregulation of anti-inflammatory factors (Arg-1 and IL-10) via the NF-κB pathway. In summary, a ROS-scavenging hyaluronic acid (HA) derivative was developed as the nanocarrier for Sin delivery to simultaneously remodel the oxidative/inflammatory microenvironment in RA, which opens up new horizons for the development of therapeutic polymers and the combined therapeutic strategies.

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Section: Resultsmentioning

confidence: 97%

Section: Study Of In Vitro Anti-inflammatory Effects and Mechanismsmentioning

confidence: 90%

Drug in Therapeutic Polymer: Sinomenine-Loaded Oxidation-Responsive Polymeric Nanoparticles for Rheumatoid Arthritis Treatment

Shang,

Sun,

Zhang

et al. 2023

ACS Appl. Mater. Interfaces

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“…Due to the lack of blood supply to adult articular cartilage, 29,30 administration of drugs or probes for arthritis treatment or imaging is often limited to local injection into the articular cavity, [31][32][33] which has many side effects including pain and catastrophic joint infection. In contrast, the cartilage lesion of HO is highly vascular, 20,34 which creates perfect conditions for the systemic application of our probe to cartilage via blood vessels.…”

Section: Discussionmentioning

confidence: 99%

Early diagnosis of heterotopic ossification with a NIR fluorescent probe by targeting type II collagen

Wang

et al. 2023

J. Mater. Chem. B

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“…For improving the efficacy of delivered drugs using engineered materials: 1) the materials used for drug delivery should have a long retention time at the target site [60] and 2) the drug should be gradually released over time. [61][62][63][64] After demonstrating the former by the non-linear directional propulsion of microrobots and their subsequent pinning on epithelial tissues, we sought to confirm that DEX-loaded microrobots can sustainably activate immune cells by controlled drug release. To do so, we measured the cumulative release of the drug from the micro robots and estimated the drug-loading capacity of a single microrobot.…”

Section: In Vitro Macrophage Polarization By Drug Release From Micror...mentioning

confidence: 99%

Bubble‐Based Microrobots with Rapid Circular Motions for Epithelial Pinning and Drug Delivery

Lee

Raj

Thome

et al. 2023

Small

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Remotely powered microrobots are proposed as next‐generation vehicles for drug delivery. However, most microrobots swim with linear trajectories and lack the capacity to robustly adhere to soft tissues. This limits their ability to navigate complex biological environments and sustainably release drugs at target sites. In this work, bubble‐based microrobots with complex geometries are shown to efficiently swim with non‐linear trajectories in a mouse bladder, robustly pin to the epithelium, and slowly release therapeutic drugs. The asymmetric fins on the exterior bodies of the microrobots induce a rapid rotational component to their swimming motions of up to ≈150 body lengths per second. Due to their fast speeds and sharp fins, the microrobots can mechanically pin themselves to the bladder epithelium and endure shear stresses commensurate with urination. Dexamethasone, a small molecule drug used for inflammatory diseases, is encapsulated within the polymeric bodies of the microrobots. The sustained release of the drug is shown to temper inflammation in a manner that surpasses the performance of free drug controls. This system provides a potential strategy to use microrobots to efficiently navigate large volumes, pin at soft tissue boundaries, and release drugs over several days for a range of diseases.

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Intra-articular injection of flavopiridol-loaded microparticles for treatment of post-traumatic osteoarthritis

Cited by 10 publications

References 46 publications

Drug in Therapeutic Polymer: Sinomenine-Loaded Oxidation-Responsive Polymeric Nanoparticles for Rheumatoid Arthritis Treatment

Drug in Therapeutic Polymer: Sinomenine-Loaded Oxidation-Responsive Polymeric Nanoparticles for Rheumatoid Arthritis Treatment

Early diagnosis of heterotopic ossification with a NIR fluorescent probe by targeting type II collagen

Bubble‐Based Microrobots with Rapid Circular Motions for Epithelial Pinning and Drug Delivery

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