Intra-arterial administration of cell-based biological agents for ischemic stroke therapy

Spiliοpoulos, Stavros; Festas, Georgios; Reppas, Lazaros; Brountzos, Elias

doi:10.1080/14712598.2019.1566454

Cited by 7 publications

(9 citation statements)

References 91 publications

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“…e main cause of death and disability after ischemic stroke is caused by decreased neuroprotection and neuroregeneration in the ischemic area [17]. Free radicals and Evidence-Based Complementary and Alternative Medicine calcium overload are widely studied mechanisms in the pathological processes of ischemic damage, especially at the stage of ischemia reperfusion [18][19][20].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway

Yang

Cen

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Context. About 15 million people worldwide suffer strokes each year and 5 million people are left with permanent disabilities which is due to the loss of local blood supply to the brain, resulting in a neurologic deficit. Panax notoginseng (Bruk.) F. H. Chen (Araliaceae) is a traditional Chinese herbal medicine widely used in the treatment of cardio-cerebrovascular diseases. Objective. This study investigated whether Panax notoginseng saponins (PNS) extracted from Panax notoginseng (Bruk.) F. H. Chen played a neuroprotective role by affecting the EGFR/PI3K/AKT pathway in oxygen-glucose deprived (OGD) SH-SY5Y cells. Materials and Methods. Different groups of OGD SH-SY5Y cells were treated with varying doses of PNS, PNS + AG1478 (a specific inhibitor of EGFR), or AG1478 for 16 hours. CCK8, Annexin V-FITC/PI apoptosis analysis, and LDH release analysis were used to determine cell viability, apoptosis rate, and amounts of LDH. Quantitative real-time PCR (q-RT-PCR) and western blotting were used to measure mRNA and proteins levels of p-EGFR/EGFR, p-PI3K/PI3K, and p-AKT/AKT in SH-SY5Y cells subjected to OGD. Results. PNS significantly enhanced cell viability, reduced apoptosis, and weakened cytotoxicity by inhibiting the release of LDH. The mRNA expression profiles of EGFR, PI3K, and AKT showed no difference between model and other groups. Additionally, ratios of p-EGFR, p-PI3K, and p-AKT to EGFR, PI3K, and AKT proteins expression, respectively, all increased significantly. Conclusions. These findings indicate that PNS enhanced neuroprotective effects by activating the EGFR/PI3K/AKT pathway and elevating phosphorylation levels in OGD SH-SY5Y cells.

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Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway

Yang

Cen

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…As we discussed above, IN route showed many advantages over current used administration route: the IV route has a poor lesion site transplantation rate 69 , 70 , 71 ; the topical injection is highly invasive and the frequency of dosage in topical injection was constrained 77 , 145 , 160 ; other administration routes like intra-arterial (IA) or intraperitoneal injection (IP) also fail to realize high CNS delivery efficiency, and both are relatively invasive 161 , 162 . On the contrary, IN route could provide a direct nose-to-brain delivery and a high brain graft rate for stem cells or EVs.…”

Section: Future Perspective For In Administration Of Stem Cells or Ev...mentioning

confidence: 99%

New idea to promote the clinical applications of stem cells or their extracellular vesicles in central nervous system disorders: Combining with intranasal delivery

Jiang

et al. 2022

Acta Pharmaceutica Sinica B

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“…Therefore, IV delivery needs a large number of cells to be injected into patients, which may increase potential side effects and the cost of the treatment ( 79 ). As the number of IA catheter-based interventions depending on stroke therapy characteristics (such as mechanical thrombectomy and catheter-directed thrombolysis for patients with penumbra) is constantly increasing, it seems that IA cell injection is ideally suited in the specific setting ( 80 , 81 ). The arterial route has a high transplantation rate, does not pass through other organs, reaches the cerebral cortex and peripheries of the lesion directly and quickly, expresses glial and neuronal markers, and induces faster improvement of neurological function in animals ( 56 ).…”

Section: The Role and Research Progress Of Msc Transplantation In The...mentioning

confidence: 99%

The role of mesenchymal stem cell transplantation for ischemic stroke and recent research developments

et al. 2022

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Ischemic stroke is a common cerebrovascular disease that seriously affects human health. However, most patients do not practice self-care and cannot rely on the current clinical treatment for guaranteed functional recovery. Stem cell transplantation is an emerging treatment studied in various central nervous system diseases. More importantly, animal studies show that transplantation of mesenchymal stem cells (MSCs) can alleviate neurological deficits and bring hope to patients suffering from ischemic stroke. This paper reviews the biological characteristics of MSCs and discusses the mechanism and progression of MSC transplantation to provide new therapeutic directions for ischemic stroke.

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Intra-arterial administration of cell-based biological agents for ischemic stroke therapy

Cited by 7 publications

References 91 publications

In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway

In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway

New idea to promote the clinical applications of stem cells or their extracellular vesicles in central nervous system disorders: Combining with intranasal delivery

The role of mesenchymal stem cell transplantation for ischemic stroke and recent research developments

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