Intra-annual variability of hepatic ethoxyresorufin-O-deethylase activity in freshwater bream <i>Abramis brama</i>

Yurchenko, Victoria; Morozov, Alexey A.

doi:10.1080/09291016.2018.1484871

Cited by 3 publications

(3 citation statements)

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“…Biometric indices BCF and HSI are reliable indicators of individual fitness and long-term changes of fish health and also, parameters of a high value that mirror the water quality [46]. Decreasing weight of somatic tissue and consequently relatively lower BCF occurs in spring, due to differentiation and growth of reproductive tissue requiring energy reserves during pre-spawning period [47]. BCF values from our study, therefore suggest good nutritional state with well-balanced metabolic expenditures of the fish.…”

Section: The Status and Condition Of A Brama Populationsmentioning

confidence: 53%

“…Moreover, seasonal variations of the enzyme activity in liver should also be considered, since it was shown that GST and CAT in different fish species are the highest after the spawning period, which reflects reorganizations in fish metabolism during the reproductive cycle [58]. Seasonal variability in hepatic CYP1A activity (measured as EROD activity), was also shown in A. brama by Yurchenko and Morozov [47], with lower values during the spring in comparison to other seasons. Lack of induction of mgst3a in our study might also result from low expression of nrf2a, since GSTs are among Nrf2 target genes [61].…”

Section: Adaptive Responses In a Brama Exposed To Chemical Stressmentioning

confidence: 99%

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Fish biomarkers from a different perspective: evidence of adaptive strategy of Abramis brama (L.) to chemical stress

et al. 2020

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Background: A prerequisite for long-term survival of populations under multi-stress conditions is their capacity to set up efficient adaptive strategies. However, changes in the activity of molecular biomarkers have been for decades considered as early signals of the deterioration of the fish health and evidence of stress-related adverse biological effects. The aim of this study was to show that such changes actually represent adaptive response of fish to chemical stress. Gene expression and enzyme activity level in liver and brain of specimens from two populations of Abramis brama from contrasted habitats (nature reserve and urban) were examined. Selected parameters included biomarkers of general stress, antioxidative defence, xenobiotic metabolism, endocrine disruption, glucose homeostasis, iron homeostasis, and neurotoxicity. Results: Exposure of A. brama population from urban area to chemical stress was confirmed by assessment of chronic toxic pressure at fish habitats using Toxic Unit approach. The most pronounced response to chemical stress is observed through the activation of antioxidative defence mechanisms in brain and liver at gene and enzyme activity level, high biotransformation capacity of liver, and activation of mechanisms that will meet energy demands and compensate for the metabolic costs of the response to toxicants (higher expression of genes related to glucose homeostasis in the exposed population). Higher hepatosomatic index in the exposed population implies liver hypertrophy due to increased functional load caused by pollution. Body condition factors indicate good overall condition of both fish populations and confirm high efficacy of mobilized adaptation mechanisms in the exposed population. Conclusions: The study provided the first data on basal expression of a number of genes in A. brama, potentially valuable for biomonitoring studies in absence of clear pollution gradient and/or reference sites (conditions). The study highlights importance of newly identified roles of various genes and proteins, typically considered as biomarkers of effects, and shows that changes in these parameters do not necessarily indicate the deterioration of the fish health. Such changes should be considered as adaptive response to chemical stress, rather than direct proof of ecological impact of pollution in situ.

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Section: The Status and Condition Of A Brama Populationsmentioning

confidence: 53%

Section: Adaptive Responses In a Brama Exposed To Chemical Stressmentioning

confidence: 99%

Fish biomarkers from a different perspective: evidence of adaptive strategy of Abramis brama (L.) to chemical stress

et al. 2020

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“…The indices of physical conditions are considered to positively correlate with the content of lipids in the organism of fish (Salam & Davies, 1994;McPherson et al, 2011;Pradhan et al, 2015;Ahmed & Sheikh, 2017). In bream, Fulton's indices of physiological condition may vary within 1.17-3.55 (Kangur, 1996;Tierney et al, 1999;Khristenko & Kotovska, 2017;Flerova et al, 2019aFlerova et al, , 2019bFlerova et al, , 2020, and Clark's indices -1.25-3.29 (Chuiko et al, 2007;Lapirova et al, 2017;Flerova et al, 2019aFlerova et al, , 2019bFlerova et al, , 2020Payuta et al, 2019;Yurchenko & Morozov, 2019).…”

Section: Discussionmentioning

confidence: 99%

Impact of habitation conditions on metabolism in the muscles, liver, and gonads of different sex and age groups of bream

Payuta

Флерова

2021

Regul. Mech. Biosyst.

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Impact of the factors of the aquatic environment is an inevitable aspect of the life of fish as poikilothermic animals and provokes responses in their organisms. The study focused on determining peculiarities in the composition of the metabolic products in the tissues of different age and sex groups of common bream Abramis brama (L.) depending on the living conditions in the water reservoirs of the Upper Volga. The fish were captured in the fattening period in summer and autumn, measured, weighed, identifying sex, maturity stage of the gonads and age. In the muscles, liver and gonads of bream, we analyzed the contents of water, dry matter, lipids, protein, ash and carbohydrates using the standard techniques. The contents of biochemical components in the organism of bream were to a higher degree determined by the peculiarities of the living conditions rather than sex and age of the individuals. In the muscles and liver of bream living in the conditions of increased water temperature and low concentration of oxygen, the concentrations of protein and ash were lower. In the same tissues of bream from the water bodies with high concentrations of pollutants in the water and benthic deposits, we found increased fat content. The muscles of male bream contained less protein and more carbohydrates than females and juvenile individuals, and the muscles of juveniles had less fat. The environmental factors had greater influence on the content of the metabolic products in the liver of bream than sex, and only females had higher ash content than males. The testes had more fat and less protein than the ovaries. During the transition of the gonads from maturity stage II to III, the contents of dry matter and lipids in them decreased. We determined that the environmental factors have stronger impact on biochemical parameters in the tissues of bream than age, because we found no general age patterns in the dynamics of biochemical components, except the liver, in which the highest content of organic compounds was found in individuals aged 6 to 10 years. Water heated by the Hydro Power Plant positively influenced the young bream, as indicated by higher content of metabolic products in individuals aged 5+ than older groups and bream from the surveyed water bodies. Monitoring of the physiological condition of bream focusing on the contents of metabolic products in the tissues may provide necessary data for successful regulation of bream populations in the water reservoirs of the Upper Volga.

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Morphological and physiological traits of the mesonephros in a freshwater fish, grayling Thymallus thymallus

Флерова

Morozov

Bogdanova

et al. 2019

Regul. Mech. Biosyst.

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The study presents new data on the structural and functional organization of the mesonephros of the grayling Thymallus thymallus (Linnaeus, 1758). Adult grayling were sampled in the middle course of the Unya River, a tributary of the Pechora River (Komi Republic, Russia). The mesonephros of the grayling, as of other freshwater fishes, is composed by nephrons, blood vessels and hematopoietic tissue forming the renal interstice. In the interstice, сells with a radial vesicle array and chloride cells were discovered; the latter were mostly localized near the renal tubules. The degree of the interstice development in the mesonephros of the grayling was determined. New data on the ultrastructure of leukocytes, cells with a radial vesicle array, chloride cells, and nephron segments were obtained. A lack of mesangial cells, a small number of podocytes, and a thin basement membrane were observed on the sections of a renal corpuscle, being characteristic features of the ultrathin organization of the mesonephros in freshwater members of Salmoniformes and Esociformes. In the grayling’s nephrons, no neck segment was found, which was reported earlier for several species, including mammals. On the sections of proximal tubules, the ciliated cells were rare, and large amounts of the tubular-vesicular network in the zone of endocytosis of the type II epithelial cells were observed. On the sections of distal tubules, short blade-shaped cytoplasmic processes, with large numbers of invaginations of cytoplasmic membrane, were found. On the basis of the distinctive ultrastructure features mentioned above, the inference that grayling show the cytological markers of adaptation to euryhalinity was made. Thus, the results contribute to the knowledge of mesonephros development in fishes during their life history. From the species protection standpoint, our study provides baseline data on a WBC differential in the mesonephros as well as superoxide dismutase, catalase, glutathione S-transferase, and ethoxyresorufin-O-deethylase activities, which can be used in further studies addressing the health status of grayling populations.

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Intra-annual variability of hepatic ethoxyresorufin-O-deethylase activity in freshwater bream Abramis brama

Cited by 3 publications

References 27 publications

Fish biomarkers from a different perspective: evidence of adaptive strategy of Abramis brama (L.) to chemical stress

Fish biomarkers from a different perspective: evidence of adaptive strategy of Abramis brama (L.) to chemical stress

Impact of habitation conditions on metabolism in the muscles, liver, and gonads of different sex and age groups of bream

Morphological and physiological traits of the mesonephros in a freshwater fish, grayling Thymallus thymallus

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