Intra- and interspecies relatedness ofYersinia pestis by DNA hybridization and its relationship toYersinia pseudotuberculosis

Bercovier, Hervé; Mollaret, H.H.; Alonso, J.M.; Brault, J; Fanning, G R; Steigerwalt, Arnold G.; Brenner, Don J.

doi:10.1007/bf02605861

Cited by 147 publications

(77 citation statements)

References 20 publications

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“…Being strictly specific, the MAb-Fib could be useful for differentiation between Y. pestis and Y. pseudotuberculosis -whose high level of genome homology has been shown previously [34]. Furthermore, Y. pseudotuberculosis was reported to form a capsule and it is unclear whether both Yersinia spp.…”

Section: Discussionmentioning

confidence: 96%

Development, characterisation and diagnostic application of monoclonal antibodies against Yersinia pestis fibrinolysin and coagulase

Feodorova

Devdariani

2000

Journal of Medical Microbiology

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A library of monoclonal antibodies (MAbs) which recognised different epitopes of Yersinia pestis fibrinolysin (Fib) was developed. These MAbs were species-specific and demonstrated no cross-reaction in indirect immunofluorescence tests (IIFT) with other gram-negative bacteria possessing plasminogen activator activity. All the MAbs provided equally high levels of immunofluorescence with pPst þ Y. pestis strains cultivated at 378C and at 288C. In all cases, the MAbs inhibited both fibrinolytic and coagulase (Coag) activities of Y. pestis in Fib-activity inhibition and coagulase-activity inhibition reactions, and reacted with 35-and 37-kDa proteins of Y. pestis in immunoblotting, demonstrating bifunctional activity possibly similar to the properties of MAbs produced by hybrid hybridomas. On the basis of these and earlier studies, the immunochemical identity of Fib and Coag, two distinct subunits of a bifunctional fusion protein whose specific functional activity depends upon the temperature factor, was established. A new rapid, cheap, strictly specific and safe dot-ELISA based on the use of MAb against Y. pestis Fib (MAb-Fib) for reliable identification of Y. pestis strains was developed. This technique has great advantages over monoclonal diagnostic kits based on the use of MAb against Y. pestis fraction I (FI) because it allows detection of plague bacilli grown at 378C as well as at 288C. This dot-ELISA will be valuable as a clinical diagnostic tool and might be applicable to field studies and plague surveillance.

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Section: Discussionmentioning

confidence: 96%

Development, characterisation and diagnostic application of monoclonal antibodies against Yersinia pestis fibrinolysin and coagulase

Feodorova

Devdariani

2000

Journal of Medical Microbiology

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“…Given the radical differences in ecology and natural history between Y. pestis and Y. pseudotuberculosis, it is surprising that they are in fact very closely related subspecies (Bercovier et al 1980;Ibrahim et al 1993). Population genetics analyses indicate that fully virulent Y. pestis strains worldwide constitute a clonal group with eight genomic branches separated by minor sequence differences (Achtman et al 2004).…”

Section: Y Pestis a Recently Emerged Clone Of Y Pseudotuberculosismentioning

confidence: 99%

Yersinia pestis Biofilm in the Flea Vector and Its Role in the Transmission of Plague

Hinnebusch¹,

Erickson²

2008

Current Topics in Microbiology and Immunology

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Transmission by fleabite is a relatively recent evolutionary adaptation of Yersinia pestis, the bacterial agent of bubonic plague. To produce a transmissible infection, Y. pestis grows as an attached biofilm in the foregut of the flea vector. Biofilm formation both in the flea foregut and in vitro is dependent on an extracellular matrix (ECM) synthesized by the Yersinia hms gene products. The hms genes are similar to the pga and ica genes of Escherichia coli and Staphylococcus epidermidis, respectively, that act to synthesize a poly-β-1,6-N-acetyl-dglucosamine ECM required for biofilm formation. As with extracellular polysaccharide production in many other bacteria, synthesis of the Hms-dependent ECM is controlled by intracellular levels of cyclic-di-GMP. Yersinia pseudotuberculosis, the food-and water-borne enteric pathogen from which Y. pestis evolved recently, possesses identical hms genes and can form biofilm in vitro but not in the flea. The genetic changes in Y. pestis that resulted in adapting biofilm-forming capability to the flea gut environment, a critical step in the evolution of vector-borne transmission, have yet to be identified. During a flea bite, Y. pestis is regurgitated into the dermis in a unique biofilm phenotype, and this has implications for the initial interaction with the mammalian innate immune response.

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“…However, phenotypically distinct strains may be closely related by genotypic criteria and may belong to the same genospecies (Bercovier et al, 1980;Hartl & Dykhuizen, 1984). Also, phenotypically close strains (biogroups) may belong to different genospecies, like Klebsiella pneumoniae and Enterobacter aerogenes (Brenner, 1984), for example.…”

Section: Introductionmentioning

confidence: 99%

Phylogeny of the Enterobacteriaceae based on genes encoding elongation factor Tu and F-ATPase β-subunit

Paradis

Boissinot

Paquette³

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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The phylogeny of enterobacterial species commonly found in clinical samples was analysed by comparing partial sequences of their elongation factor Tu gene (tuf ) and of their F-ATPase b-subunit gene (atpD). An 884 bp fragment for tuf and an 884 or 871 bp fragment for atpD were sequenced for 96 strains representing 78 species from 31 enterobacterial genera. The atpD sequence analysis exhibited an indel specific to Pantoea and Tatumella species, showing, for the first time, a tight phylogenetic affiliation between these two genera. Comprehensive tuf and atpD phylogenetic trees were constructed and are in agreement with each other. Monophyletic genera are Cedecea, Edwardsiella, Proteus, Providencia, Salmonella, Serratia, Raoultella and Yersinia. Analogous trees based on 16S rRNA gene sequences available from databases were also reconstructed. The tuf and atpD phylogenies are in agreement with the 16S rRNA gene sequence analysis, and distance comparisons revealed that the tuf and atpD genes provide better discrimination for pairs of species belonging to the family Enterobacteriaceae. In conclusion, phylogeny based on tuf and atpD conserved genes allows discrimination between species of the Enterobacteriaceae. INTRODUCTIONMembers of the family Enterobacteriaceae are facultatively anaerobic, Gram-negative rods that are catalase-positive and oxidase-negative (Brenner, 1984). They are found in soil, water and plants, and also in animals ranging from insects to humans. Many enterobacteria are opportunistic pathogens. In fact, members of this family are responsible for about 50 % of nosocomial infections in the US (Brenner, 1984). Therefore, this family is of considerable clinical importance.The major classification studies on the family Enterobacteriaceae were based on phenotypic traits (Brenner et al., , 1999Dickey & Zumoff, 1988;Farmer et al., 1980Farmer et al., , 1985a such as biochemical reactions and physiological characteristics. However, phenotypically distinct strains may be closely related by genotypic criteria and may belong to the same genospecies (Bercovier et al., 1980;Hartl & Dykhuizen, 1984). Also, phenotypically close strains (biogroups) may belong to different genospecies, like Klebsiella pneumoniae and Enterobacter aerogenes (Brenner, 1984), for example. Consequently, identification and classification of certain species may be ambiguous with techniques based on phenotypic tests (Janda et al., 1999;Kitch et al., 1994;Sharma et al., 1990).More advances in the classification of members of the family Enterobacteriaceae have come from DNA-DNA hybridization studies (Brenner et al., , 1986(Brenner et al., , 1993Farmer et al., 1980Farmer et al., , 1985aIzard et al., 1981;Steigerwalt et al., 1976). The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, tuf and atpD gene sequences obtained in this study are listed in Furthermore, the phylogenetic significance of bacterial classification based on 16S rRNA gene sequences has been recognized by many workers Wayne et al., 1987). However, members of the family En...

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Intra- and interspecies relatedness ofYersinia pestis by DNA hybridization and its relationship toYersinia pseudotuberculosis

Cited by 147 publications

References 20 publications

Development, characterisation and diagnostic application of monoclonal antibodies against Yersinia pestis fibrinolysin and coagulase

Development, characterisation and diagnostic application of monoclonal antibodies against Yersinia pestis fibrinolysin and coagulase

Yersinia pestis Biofilm in the Flea Vector and Its Role in the Transmission of Plague

Phylogeny of the Enterobacteriaceae based on genes encoding elongation factor Tu and F-ATPase β-subunit

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