Intra‐ and interlaboratory variability in acute toxicity tests with glochidia and juveniles of freshwater mussels (unionidae)

Wang, Ning; Augspurger, Tom; Barnhart, M. Chris; Bidwell, Joseph R.; Cope, W. Gregory; Dwyer, F. James; Geis, Steve; Greer, I. Eugene; Ingersoll, Chris G.; Kane, Cynthia M.; May, Thomas W.; Neves, Richard J.; Newton, Teresa J.; Roberts, Andy D.; Whites, David W.

doi:10.1897/06-520r.1

Cited by 43 publications

(72 citation statements)

References 16 publications

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“…A random subsample of 30 glochidia from each female was tested for viability with sodium chloride (Wang et al 2007) to confirm their infection potential. Glochidia from six gravid A. woodiana females (mean ± SD of shell length 184 ± 14 mm) with a glochidia viability of over 90% were pooled and used for inoculations.…”

Section: Experimental Test Of Host Specificitymentioning

confidence: 99%

The role of host specificity in explaining the invasion success of the freshwater mussel Anodonta woodiana in Europe

et al. 2011

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Several freshwater mussel species represent some of the most problematic invasive species and have considerably altered ecosystems worldwide. Their invasion potential has been partially attributed to their free-living larvae, which have a high dispersal capability. We investigated the invasion potential of Anodonta (Sinanodonta) woodiana, a species of East Asian unionid mussel established worldwide despite having an obligatory parasitic stage (glochidium), which must encyst on host fish. The invasion success of A. woodiana has been attributed to the success of worldwide introductions of its sympatric fish hosts. We experimentally found, however, that A. woodiana is a broad host generalist, which can complete its development on all eight fish species tested, both coinvasive and native. Subsequently, we used a data on the occurrence and relative abundance of potential hosts in river habitats in the Czech Republic to project scenarios of the effect of host availability on A. woodiana invasion. We found that host availability does not constitute a major limit for A. woodiana to colonise most aquatic habitats in Central Europe. In addition, we investigated seasonal dynamics of A. woodiana reproduction and did not detect any limitations of its reproduction by ambient water temperatures typical of a Central European lowland river. Consequently, we predict that A. woodiana may further increase the speed and range of its invasion and we discuss possible consequences to native habitats and communities, especially to the endangered species of unionid mussels.

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Section: Experimental Test Of Host Specificitymentioning

confidence: 99%

The role of host specificity in explaining the invasion success of the freshwater mussel Anodonta woodiana in Europe

et al. 2011

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“…Glochidia were obtained by flushing the marsupium with water using a syringe (methods in Dodd et al, 2005). A random subsample of 30 glochidia was tested for viability with sodium chloride according to Wang et al (2007a). Two gravid females (length 93.0, 86.0 mm) that had glochidial viability over 90% were used for inoculation.…”

Section: Toxicity Testingmentioning

confidence: 99%

Effects of nitrate nitrogen pollution on Central European unionid bivalves revealed by distributional data and acute toxicity testing

Douda

2009

Aquatic Conservation

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ABSTRACT1. Studies from Central Europe have shown a relationship between the impaired population status of threatened freshwater mussel species and elevated nitrate nitrogen (N-NO À 3 ) concentrations in running waters. 2. Causal mechanisms, however, remain unknown, and no experimental data or comprehensive studies involving more species are available, which causes uncertainty in prioritizing conservation actions.3. This study uses both descriptive and experimental approaches to identify the effects of nitrates on freshwater mussels and demonstrates the need for integrating different research methods for development of conservation strategies for threatened species.4. Spatial co-occurrence of five native freshwater mussel species (Anodonta anatina, Pseudanodonta complanata, Unio pictorum, Unio tumidus, Unio crassus) and N-NO À 3 concentrations were examined in a 7th-order river catchment (Luzˇnice River, Czech Republic) with anthropogenically-induced increasing N-NO À 3 levels and declining populations of these species during the 20th century.5. Acute toxicity of N-NO À 3 was then estimated for artificially reared juveniles of A. anatina and U. crassus using both lethal and sublethal test endpoints.6. Results showed that the probability of occurrence of all species was significantly reduced in reaches with elevated N-NO À 3 levels. 7. In contrast, the results of toxicity testing revealed that the juvenile stages of the two tested species were less sensitive to N-NO À 3 than most previously tested freshwater macroinvertebrates. The detected 96-h median lethal N-NO À 3 concentrations were two orders of magnitude higher than the limits derived from distributional data. 8. Despite the probable absence of a direct negative effect of N-NO À 3 on freshwater mussel populations, N-NO À 3 has potential to be used as an effective indicator of biotope conditions. Identification of causal mechanisms responsible for the observed relationship between unionids and N-NO À 3 will require further research.

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“…Juvenile mussels (fatmucket Lampsilis siliquoidea) tested had an age of one week (for test 1) or two months (for test 2) and were obtained from Missouri State University (Springfield, MO; see Wang et al [24] for a description of methods used to culture the mussels). The test organisms were acclimated to test water and temperature for at least 24 h before the start of the toxicity tests [25][26][27][28][29].…”

Section: Test Organismsmentioning

confidence: 99%

Toxicity of silicon carbide nanowires to sediment‐dwelling invertebrates in water or sediment exposures

Mwangi

Wang

Ritts

et al. 2011

Enviro Toxic and Chemistry

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Silicon carbide nanowires (SiCNW) are insoluble in water. When released into an aquatic environment, SiCNW would likely accumulate in sediment. The objective of this study was to assess the toxicity of SiCNW to four freshwater sediment-dwelling organisms: amphipods (Hyalella azteca), midges (Chironomus dilutus), oligochaetes (Lumbriculus variegatus), and mussels (Lampsilis siliquoidea). Amphipods were exposed to either sonicated or nonsonicated SiCNW in water (1.0 g/L) for 48 h. Midges, mussels, and oligochaetes were exposed only to sonicated SiCNW in water for 96 h. In addition, amphipods were exposed to sonicated SiCNW in whole sediment for 10 d (44% SiCNW on dry wt basis). Mean 48-h survival of amphipods exposed to nonsonicated SiCNW in water was not significantly different from the control, whereas mean survival of amphipods exposed to sonicated SiCNW in two 48-h exposures (0 or 15% survival) was significantly different from the control (90 or 98% survival). In contrast, no effect of sonicated SiCNW was observed on survival of midges, mussels, or oligochaetes. Survival of amphipods was not significantly reduced in 10-d exposures to sonicated SiCNW either mixed in the sediment or layered on the sediment surface. However, significant reduction in amphipod biomass was observed with the SiCNW either mixed in sediment or layered on the sediment surface, and the reduction was more pronounced for SiCNW layered on the sediment. These results indicated that, under the experimental conditions, nonsonicated SiCNW in water were not acutely toxic to amphipods, sonicated SiCNW in water were acutely toxic to the amphipods, but not to other organisms tested, and sonicated SiCNW in sediment affected the growth but not the survival of amphipods.

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Intra‐ and interlaboratory variability in acute toxicity tests with glochidia and juveniles of freshwater mussels (unionidae)

Cited by 43 publications

References 16 publications

The role of host specificity in explaining the invasion success of the freshwater mussel Anodonta woodiana in Europe

The role of host specificity in explaining the invasion success of the freshwater mussel Anodonta woodiana in Europe

Effects of nitrate nitrogen pollution on Central European unionid bivalves revealed by distributional data and acute toxicity testing

Toxicity of silicon carbide nanowires to sediment‐dwelling invertebrates in water or sediment exposures

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