Intimin from enteropathogenic Escherichia coli mediates remodelling of the eukaryotic cell surface

Phillips, Alan D.; Girón, Jorge A.; Hicks, Susan; Dougan, Gordon; Frankel, Gad

doi:10.1099/00221287-146-6-1333

Cited by 40 publications

(23 citation statements)

References 45 publications

(5 reference statements)

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“…Phillips and coworkers have established an IVOC from pediatric biopsy specimens of duodenum mounted in a modified MicroSnapwell system specifically adapted for use with 2-to 3-mmdiameter biopsy samples. This IVOC system has been used mainly to study the mechanisms of virulence of ETEC (839), EPEC (840)(841)(842)(843)(844)(845)(846)(847)(848)(849)(850)(851), EHEC (852)(853)(854)(855)(856)(857), EAEC (507,858), and Salmonella (392) and the action of Stx toxins (641). Other IVOCs have been used for studies on C. jejuni (859), EPEC (692,860,861), EAEC (645,(862)(863)(864), and Shigella (865) pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Knutton et al (464) observed the presence of concentrated cellular F actin at the sites of attachment in fully differentiated Caco-2 cells and reported that after effacement of the microvillus, tEPEC bacteria are seen localized on a pedestal-like structure. tEPEC BFP, adhesin intimin, and several translocated T3SS effectors have been identified as intimately adhering to fully differentiated Caco-2 cells (603,604). tEPEC T3SS effectors EspA, EspB, and EspD have been shown to be required for the formation of the A/E lesions in fully differentiated Caco-2 cells (605).…”

Section: Structural and Functional Injuriesmentioning

confidence: 99%

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Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Moal

Servin

2013

Microbiol Mol Biol Rev

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SUMMARY Hosts are protected from attack by potentially harmful enteric microorganisms, viruses, and parasites by the polarized fully differentiated epithelial cells that make up the epithelium, providing a physical and functional barrier. Enterovirulent bacteria interact with the epithelial polarized cells lining the intestinal barrier, and some invade the cells. A better understanding of the cross talk between enterovirulent bacteria and the polarized intestinal cells has resulted in the identification of essential enterovirulent bacterial structures and virulence gene products playing pivotal roles in pathogenesis. Cultured animal cell lines and cultured human nonintestinal, undifferentiated epithelial cells have been extensively used for understanding the mechanisms by which some human enterovirulent bacteria induce intestinal disorders. Human colon carcinoma cell lines which are able to express in culture the functional and structural characteristics of mature enterocytes and goblet cells have been established, mimicking structurally and functionally an intestinal epithelial barrier. Moreover, Caco-2-derived M-like cells have been established, mimicking the bacterial capture property of M cells of Peyer's patches. This review intends to analyze the cellular and molecular mechanisms of pathogenesis of human enterovirulent bacteria observed in infected cultured human colon carcinoma enterocyte-like HT-29 subpopulations, enterocyte-like Caco-2 and clone cells, the colonic T84 cell line, HT-29 mucus-secreting cell subpopulations, and Caco-2-derived M-like cells, including cell association, cell entry, intracellular lifestyle, structural lesions at the brush border, functional lesions in enterocytes and goblet cells, functional and structural lesions at the junctional domain, and host cellular defense responses.

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Section: Discussionmentioning

confidence: 99%

Section: Structural and Functional Injuriesmentioning

confidence: 99%

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Moal

Servin

2013

Microbiol Mol Biol Rev

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“…It is proposed that the formation of AE lesions results in a reduction in the absorptive capacity of the intestinal mucosa, which inevitably leads to disruption of the electrolyte balance and subsequently to diarrhea. AE lesions have been demonstrated both in vivo (in biopsy specimens taken from infants with diarrhea) (24,148,173) and in vitro with a range of cell lines and organ explants (56,106,134). AE lesion formation is dependent on a number of physiological and environmental conditions (146) and is optimal in early to midlogarithmic growth at 37°C (AE lesions are not induced at 28°C).…”

Section: Introductionmentioning

confidence: 99%

Virulence of EnteropathogenicEscherichia coli, a Global Pathogen

et al. 2003

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Enteropathogenic Escherichia coli (EPEC) remains an important cause of diarrheal disease worldwide. Research into EPEC is intense and provides a good virulence model of other E. coli infections as well as other pathogenic bacteria. Although the virulence mechanisms are now better understood, they are extremely complex and much remains to be learnt. The pathogenesis of EPEC depends on the formation of an ultrastructural lesion in which the bacteria make intimate contact with the host apical enterocyte membrane. The formation of this lesion is a consequence of the ability of EPEC to adhere in a localized manner to the host cell, aided by bundle-forming pili. Tyrosine phosphorylation and signal transduction events occur within the host cell at the lesion site, leading to a disruption of the host cell mechanisms and, consequently, to diarrhea. These result from the action of highly regulated EPEC secreted proteins which are released via a type III secretion system, many genes of which are located within a pathogenicity island known as the locus of enterocyte effacement. Over the last few years, dramatic increases in our knowledge of EPEC virulence have taken place. This review therefore aims to provide a broad overview of and update to the virulence aspects of EPEC

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“…In vivo studies using different animal models have demonstrated that in comparison to the wild type, intimin-deficient EHEC O157:H7 is defective in intestinal colonization and, in some models, in the induction of enteritis (9,10,15,25,26,36,42,47,50). Intimin exists in at least six phylogenetically distinct subtypes which exhibit marked se-quence divergence in their carboxyl-terminal cell-binding domains (1,18,19,39,40,47). The carboxyl-terminal domain of intimin mediates the interaction with Tir; however, interactions between intimin and cellular coreceptors, including ␤1-integrins and surface-localized nucleolin, have been described previously (20,43).…”

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confidence: 99%

Role of Intimin-Tir Interactions and the Tir-Cytoskeleton Coupling Protein in the Colonization of Calves and Lambs by Escherichia coli O157:H7

Vlisidou

Dziva

Ragione³

et al. 2006

Infect Immun

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Intimin facilitates intestinal colonization by enterohemorrhagic Escherichia coli O157:H7; however, the importance of intimin binding to its translocated receptor (Tir) as opposed to cellular coreceptors is unknown. The intimin-Tir interaction is needed for optimal actin assembly under adherent bacteria in vitro, a process which requires the Tir-cytoskeleton coupling protein (TccP/EspF U ) in E. coli O157:H7. Here we report that E. coli O157:H7 tir mutants are at least as attenuated as isogenic eae mutants in calves and lambs, implying that the role of intimin in the colonization of reservoir hosts can be explained largely by its binding to Tir. Mutation of tccP uncoupled actin assembly from the intimin-Tir-mediated adherence of E. coli O157:H7 in vitro but did not impair intestinal colonization in calves and lambs, implying that pedestal formation may not be necessary for persistence. However, an E. coli O157:H7 tccP mutant induced typical attaching and effacing lesions in a bovine ligated ileal loop model of infection, suggesting that TccP-independent mechanisms of actin assembly may operate in vivo.

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Intimin from enteropathogenic Escherichia coli mediates remodelling of the eukaryotic cell surface

Abstract: Adhesion to cultured epithelial cells by enteropathogenic Escherichia coli

Cited by 40 publications

References 45 publications

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Pathogenesis of Human Enterovirulent Bacteria: Lessons from Cultured, Fully Differentiated Human Colon Cancer Cell Lines

Virulence of EnteropathogenicEscherichia coli, a Global Pathogen

Role of Intimin-Tir Interactions and the Tir-Cytoskeleton Coupling Protein in the Colonization of Calves and Lambs by Escherichia coli O157:H7

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