Intestinal Tropism of a Betacoronavirus (
            <i>Merbecovirus</i>
            ) in Nathusius’s Pipistrelle Bat (
            <i>Pipistrellus nathusii</i>
            ), Its Natural Host

Mols, Vera C.; Lamers, Mart M.; Leijten, Lonneke; Breugem, Tim I; Bildt, Marco Wg van de; Doel, Petra B. van den; Lina, Peter H. C.; Haagmans, Bart L.; Kuiken, Thijs; Begeman, Lineke

doi:10.1128/jvi.00099-23

Cited by 5 publications

(15 citation statements)

References 58 publications

(57 reference statements)

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“…Recently, two novel bat merbecoviruses with complete genome sequenced, MOW-15-22 and PN-βCoV (designated PnNL2018B in this study), were independently reported by research teams in Russia and Netherlands 35,36 (Fig. 1a).…”

Section: Phylogenetic and Structural Analyses Suggest Ace2 Usage By M...mentioning

confidence: 84%

“…Nonetheless, the receptors for many merbecoviruses, including hedgehog coronaviruses (EriCoVs) such as HKU31, as well as several other bat coronaviruses like HKU5, MOW-15-22, and PN-βCoV remain elusive 29,[34][35][36] . Previous studies have suggested that HKU5 and HKU31 may not use DPP4 or ACE2 as their receptors, leaving their entry receptor identities to be determined.…”

Section: Introductionmentioning

confidence: 99%

“…3 MOW-15-22 and PN-βCoV are two bat merbecoviruses recently identified in P. nathusii bats, a bat species commonly found in Europe 35,36 . To avoid confusion, we designated a specific virus of PN-βCoV (Betacoronavirus sp.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have suggested that HKU5 and HKU31 may not use DPP4 or ACE2 as their receptors, leaving their entry receptor identities to be determined. MOW-15-22 and PN-βCoV are two bat merbecoviruses recently identified in P. nathusii bats, a bat species commonly found in Europe 35,36 . To avoid confusion, we designated a specific virus of PN-βCoV (Betacoronavirus sp.…”

Section: Introductionmentioning

confidence: 99%

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Multiple independent acquisitions of ACE2 usage in MERS-related coronaviruses

Ma,

Liu,

Park

et al. 2023

Preprint

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The unknown identity of the entry receptors utilized by many coronaviruses has significantly impeded our comprehensive understanding of these important pathogens. We recently reported an unexpected usage of angiotensin-converting enzyme 2 (ACE2), instead of Dipeptidyl peptidase-4 (DPP4), for cellular entry by NeoCoV and PDF-2180, close relatives of MERS-CoV that infect African bats. However, the presence and distribution of other ACE2-using merbecoviruses remain enigmatic. In this study, through sequence and structural analyses, we predicted that two newly discovered merbecoviruses infecting European Pipistrellus bats (Pipistrellus nathusii), namely MOW-15-22 and PnNL2018B, may also utilize ACE2 as their receptors. Functional profiling of 103 ACE2 orthologues from a variety of mammals confirmed that several ACE2 from bats efficiently facilitate the entry of MOW-15-22 and PnNL2018B. Conversely, no binding or entry signals for both viruses were detected when assessing seven DPP4 orthologues from humans, hedgehogs, and bats. Characterization of Pteronotus davyi (P.dav) ACE2 mediated entry of MOW-15-22 reveals a significant exogenous protease dependence, which can be dose-dependently neutralized by soluble P.dav ACE2 recombinant protein and a broadly neutralizing S2-targeting antibody. Verification of the previously reported critical ACE2 determinants forNeoCoV recognition reveals thatMOW-15-22 and PnNL2018B displayed a glycan-independent binding mode with significantly altered interaction details. This study sheds light on two additional ACE2-using merbecoviruses circulating among European bats and underscores the potential zoonotic risk associated with these viruses.

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Section: Phylogenetic and Structural Analyses Suggest Ace2 Usage By M...mentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multiple independent acquisitions of ACE2 usage in MERS-related coronaviruses

Ma,

Liu,

Park

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…MERS-CoV infection has resulted in approximately 2700 fatalities and sustained low-level local transmission in Saudi Arabia ( WHO, 2012 ). Relatives of MERS-CoV have been found in several animal species, including bats, camels, hedgehogs and pangolins ( Chen et al., 2023 ; Chu et al., 2014 ; Hemida et al., 2014 ; Lau et al., 2019 ; Luo et al., 2018 ; Mols et al., 2023 ; Shishido & Letizia, 2015 ; Speranskaya et al., 2023 ). Dromedary camels have been well-documented as intermediate hosts of MERS-CoV, and subsequent research has uncovered many relatives of MERS-CoV in bats, supporting the hypothesis that the origins and evolution of MERS-CoV trace back to bats ( Anthony et al., 2017 ; Chu et al., 2014 ; Cui et al., 2019 ; Lau et al., 2013 ; Memish et al., 2013 ).…”

mentioning

confidence: 99%