Intestinal transport and absorption of bioactive phenolic compounds from a chemically characterized aqueous extract of Athrixia phylicoides

Bowles, Sandra; Ntamo, Yonela; Malherbe, Christiaan J.; Kappo, Abidemi Paul; Louw, Johan; Muller, Christo J. F.

doi:10.1016/j.jep.2017.02.019

Cited by 12 publications

(2 citation statements)

References 20 publications

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“…After gastrointestinal digestion, the intestinal absorption of phenolic compounds has also been reported to be highly influenced by the phenolic compounds chemical structure. Bowles et al [ 15 ] studied the intestinal transport across Caco-2 cell monolayer of nine phenolic acids found in an aqueous extract of Athrixia phylicoides , concluding that p -coumaric acid presented the highest transport. Besides, Wu et al [ 16 ] reported that the absorption of caffeic acid was higher than chlorogenic acid in the Caco-2 model as well as in rat jejunum.…”

Section: Introductionmentioning

confidence: 99%

Bioavailability Assessment of Yarrow Phenolic Compounds Using an In Vitro Digestion/Caco-2 Cell Model: Anti-Inflammatory Activity of Basolateral Fraction

et al. 2022

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In this study, a combined in vitro digestion/Caco-2 model was performed with the aim to determine the phenolic compounds bioavailability of two yarrow extracts. HPLC-PAD characterisation indicated that the main components in both extracts were 3,5-dicaffeoylquinic acid (DCQA) and luteolin-7-O-glucoside. Analyses after the simulated digestion process revealed that phenolic composition was not affected during the oral phase, whereas gastric and intestinal phases represented critical steps for some individual phenolics, especially intestinal step. The transition from gastric medium to intestinal environment caused an important degradation of 3,5-DCQA (63–67% loss), whereas 3,4-DCQA and 4,5-DCQA increased significantly, suggesting an isomeric transformation within these caffeic acid derivatives. However, an approx. 90% of luteolin-7-O-glucoside was recovered after intestinal step. At the end of Caco-2 absorption experiments, casticin, diosmetin and centaureidin represented the most abundant compounds in the basolateral fraction. Moreover, this fraction presented anti-inflammatory activity since was able to inhibit the secretion of IL-1β and IL-6 pro-inflammatory cytokines. Thus, the presence in the basolateral fraction of flavonoid-aglycones from yarrow, could be related with the observed anti-inflammatory activity from yarrow extract.

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Section: Introductionmentioning

confidence: 99%

Bioavailability Assessment of Yarrow Phenolic Compounds Using an In Vitro Digestion/Caco-2 Cell Model: Anti-Inflammatory Activity of Basolateral Fraction

et al. 2022

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“…These results possibly indicated their favorable intestinal permeability and drug-likeness. In fact, the in vitro permeability for all the 17 ingredients have ever been reported using Caco-2 permeability assay (Lu et al, 2008; Liu et al, 2009; Hu et al, 2014; Bowles et al, 2017). According to the previous results (Table 1), also only DSS, PCA, PCAL, CA, DHTS I, CTS, TS I, and TS IIA showed the apparent permeability of ≥10 -6 cm/s in Caco-2 cell monolayers and were considered to be favorable for their absorption in vivo .…”

Section: Resultsmentioning

confidence: 99%

Identification of Pharmacokinetic Markers for Guanxin Danshen Drop Pills in Rats by Combination of Pharmacokinetics, Systems Pharmacology, and Pharmacodynamic Assays

et al. 2018

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This paper reported a feasibility study strategy of identifying pharmacokinetic (PK) markers for a cardiovascular herbal medicine, Guanxin Danshen drop pill (GDDP). First, quantification analysis revealed the constituent composition in the preparation by high-performance liquid chromatography (HPLC). Subsequently, physiochemical property calculation predicted the solubility and intestinal permeability of the constituents in the preparation. Furthermore, HPLC–MS analysis ascertained the absorbable ingredients and their PK properties in rat plasma. The main effective substances from the ingredients absorbed into blood and their cardiovascular effects were also predicted by systems pharmacology study, and were further confirmed by in vivo protective effects on isoprenaline-induced myocardial injury in mice. Finally, the ingredients with high content, representative structure feature, favorable PK properties, high relevant degree to myocardial ischemia (MI) issues, and validated therapeutic effects were considered as the PK markers for the preparation. Ginsenosides Rg1, Rb1, and tanshinone (TS) IIA were identified originally as PK markers for representing PK properties of GDDP. In addition, integrated PK studies were carried out according to previous reports, viz. drug concentration sum method and the AUC weighting method, to understand the in vivo process of GDDP comprehensively. The present study maybe provide a reference approach to identify PK markers for cardiovascular herbal medicines.

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Analysis of herbal bioactives

Ares

Bernal

Nozal

2021

Aromatic Herbs in Food

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Intestinal transport and absorption of bioactive phenolic compounds from a chemically characterized aqueous extract of Athrixia phylicoides

Cited by 12 publications

References 20 publications

Bioavailability Assessment of Yarrow Phenolic Compounds Using an In Vitro Digestion/Caco-2 Cell Model: Anti-Inflammatory Activity of Basolateral Fraction

Bioavailability Assessment of Yarrow Phenolic Compounds Using an In Vitro Digestion/Caco-2 Cell Model: Anti-Inflammatory Activity of Basolateral Fraction

Identification of Pharmacokinetic Markers for Guanxin Danshen Drop Pills in Rats by Combination of Pharmacokinetics, Systems Pharmacology, and Pharmacodynamic Assays

Analysis of herbal bioactives

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