Intestinal Ralstonia pickettii augments glucose intolerance in obesity

Udayappan, Shanthadevi D.; Kovatcheva‐Datchary, Petia; Bakker, Guido J.; Havik, Stefan R.; Herrema, Hilde; Cani, Patrice D.; Bouter, Kristien E.; Belzer, Clara; Witjes, Julia J.; Vrieze, Anne; Sonnaville, Noor de; Chaplin, Alice; Raalte, Daniël H. van; Aalvink, Steven; Dallinga‐Thie, Geesje M.; Heilig, Hans G.H.J.; Bergström, Göran; Meij, Suzan van der; Wagensveld, Bart A. van; Hoekstra, J. B. L.; Holleman, F.; Stroes, Erik S.G.; Groen, Albert K.; Bäckhed, Fredrik; Vos, Willem M. de; Nieuwdorp, Max

doi:10.1371/journal.pone.0181693

Cited by 58 publications

(41 citation statements)

References 43 publications

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“…Culturing experiments to validate these findings remained negative. In contrast, bacterial DNA was reported in mesenteric adipose tissue of 12 subjects with obesity belonging mostly to Ralstonia a year later [212]. Bacterial DNA was moreover detected in epicardial adipose tissue of subjects with acute coronary syndrome and stable angina, whereas none could be found in subjects with isolated mitral insufficiency [213].…”

Section: Bacterial Presence In Remote Tissuesmentioning

confidence: 90%

Gut Microbiome, Intestinal Permeability, and Tissue Bacteria in Metabolic Disease: Perpetrators or Bystanders?

Chakaroun¹,

Massier²,

Kovacs³

2020

Nutrients

170

133

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The emerging evidence on the interconnectedness between the gut microbiome and host metabolism has led to a paradigm shift in the study of metabolic diseases such as obesity and type 2 diabetes with implications on both underlying pathophysiology and potential treatment. Mounting preclinical and clinical evidence of gut microbiota shifts, increased intestinal permeability in metabolic disease, and the critical positioning of the intestinal barrier at the interface between environment and internal milieu have led to the rekindling of the “leaky gut” concept. Although increased circulation of surrogate markers and directly measurable intestinal permeability have been linked to increased systemic inflammation in metabolic disease, mechanistic models behind this phenomenon are underdeveloped. Given repeated observations of microorganisms in several tissues with congruent phylogenetic findings, we review current evidence on these unanticipated niches, focusing specifically on the interaction between gut permeability and intestinal as well as extra-intestinal bacteria and their joint contributions to systemic inflammation and metabolism. We further address limitations of current studies and suggest strategies drawing on standard techniques for permeability measurement, recent advancements in microbial culture independent techniques and computational methodologies to robustly develop these concepts, which may be of considerable value for the development of prevention and treatment strategies.

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Section: Bacterial Presence In Remote Tissuesmentioning

confidence: 90%

Gut Microbiome, Intestinal Permeability, and Tissue Bacteria in Metabolic Disease: Perpetrators or Bystanders?

Chakaroun¹,

Massier²,

Kovacs³

2020

Nutrients

170

133

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“…may be a consequence of misidentifications of these bacteria, being suggested that members of this group may be more widespread, invasive and severe than previously thought (Coman et al 2017;Daxboeck et al 2005;Ryan et al 2006). A recent study reported an association between the presence of intestinal Ralstonia pickettii and an augmented glucose intolerance in obesity (Udayappan et al, 2017). Although members of R. pickettii and R. mannitolilytica lack major virulence factors, and rarely are reported as causing infection, members of these species have been considered the most pathogenic species of the genus (Vaneechoutte et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Association between gentamicin resistance and stress tolerance in water isolates of Ralstonia pickettii and R. mannitolilytica

2018

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Members of the species Ralstonia pickettii and R. mannitolilytica, although ubiquitous and lacking major virulence factors, have been associated with nosocomial outbreaks. Tolerance to metals, antibiotics, and disinfectants may represent an advantage for their ubiquity and opportunistic pathogenic potential. In this study, we compared five strains that differed on the origin (hospital effluent, tap water, mineral water) and in the susceptibility to aminoglycosides, regarding their tolerance to metals and disinfection. The growth kinetics and biofilm formation capacity were tested in four R. pickettii strains and one R. mannitolilytica at sub-inhibitory concentrations of aminoglycosides or arsenite. The survival to UV radiation, chlorine, or hydrogen peroxide was also compared in aminoglycoside resistant and susceptible strains. Aminoglycoside-resistant strains presented a higher tolerance to arsenite than the susceptible ones and either aminoglycosides or arsenite was observed to stimulate the biofilm formation. Sub-inhibitory concentrations of the aminoglycoside gentamicin or arsenite significantly decreased the growth rate and yield, but only arsenite caused a significant increase of the lag phase. Hydrogen peroxide presented higher disinfection effectiveness against aminoglycoside susceptible than against resistant strains, an effect that was not observed for UV or chlorine. Although this conclusion needs validation based on a larger number of isolates, including clinical, the results suggest that aminoglycoside resistance may be associated with traits that influence Ralstonia spp. fitness in the environment.

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“…11 Bacterial DNA is present in mesenteric adipose tissue, reflecting an impaired intestinal barrier, and in another study in T2DM patients, Ralstonia pickettii was one of the most prevalent bacterial strains. 12 Ralstonia treated diet induced obese mice exhibited a deteriorated insulin resis tance compared with control animals, suggest ing that this bacterium reflects one candidate being potentially of relevance in T2DM. Over all, the intestinal epithelium might play a key role in health and metabolic balance, and an ex citing recent study showed that glucose itself is able to impair intestinal mucosal integrity.…”

Section: Referencesmentioning

confidence: 98%

Microbiota and diabetes: an increasingly relevant association

Tilg¹,

Grander²

2018

Polish Archives of Internal Medicine

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333field was opened for further investigations and the term "altered microbiota signature in T2D" was established.In this issue of Polish Archives of Internal Medicine (Pol Arch Intern Med), Salamon et al 6 report on a small study where they investigated fecal microbiome in patients with T1DM, T2DM, and healthy controls. Using 16S ribosomal RNA next generation sequencing, the authors observed that patients with T2DM differed with respect to their microbiome from both T1DM subjects and healthy controls. Patients with T2DM were char acterized by a higher ratio of Firmicutes to Bac teroidetes and demonstrated reduced fecal con centrations of certain Ruminococcaceae, Lachno spiraceae, Clostridiales, Bacteroides, Anaerostipes, and Roseburia, whereas the concentrations of Ruminococcus, Enterobacteriaceae and Proteobacte ria, all rather proinflammatory bacterial strains, were increased. Proteobacteria are considered pro inflammatory and might be able to drive immune responses characterized by increased interferon γ expression. 7 Concentrations of Akkermansia muciniphila, a rather well characterized commensal with mainly antidiabetic and anti inflammatory properties, 8 were increased in T2DM patients in the study by Salomon et al, 6 and the authors pro posed that accompanying metformin treatment might have caused this effect. 9 The findings by Salamon et al 6 with A muciniphila are in contrast to several other studies which have demonstrated that obesity and related metabolic dysfunction are characterized by decreased fecal concentrations of this bacterium. 10 Interestingly, the microbiota composition in T1DM subjects did not differ sig nificantly from that in healthy controls. 6 Some at tention regarding the presented results, however, is needed as authors could not provide data on di etary details and stool consistency, both factors substantially affecting gut microbiota composi tion. Furthermore, the number of analyzed pa tients was rather low (T1DM, n = 22; T2DM, n = 23; controls, n = 23). Despite these shortcomings, The human intestinal tract contains an enor mous number of bacteria, archaea, and virus es. Recent estimates announced that a ratio of 1.3 bacterial cells might exist for every 1 hu man cell. 1 Early studies believed that genes of this microbial world mainly encode functions affecting pathways favoring digestion of com plex carbohydrates or the control of immunity, whereas recent evidence supports that the mi crobiota has major functions in the regulation of metabolic pathways in health and disease. 2 It has been shown in the past years that obese human subjects exhibit a gut microbiome sig nature. 3 Two major studies from the following years showed that such a microbiome signature might also exist in humans with type 2 diabe tes mellitus (T2DM). 4,5 In the first metagenome wide association study, researchers from Chi na demonstrated an intestinal dysbiosis char acterized by a decrease in butyrate producing Roseburia intestinalis and Faecalibacterium prausnitzii. 4 When analyzing potentially associated...

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Intestinal Ralstonia pickettii augments glucose intolerance in obesity

Cited by 58 publications

References 43 publications

Gut Microbiome, Intestinal Permeability, and Tissue Bacteria in Metabolic Disease: Perpetrators or Bystanders?

Gut Microbiome, Intestinal Permeability, and Tissue Bacteria in Metabolic Disease: Perpetrators or Bystanders?

Association between gentamicin resistance and stress tolerance in water isolates of Ralstonia pickettii and R. mannitolilytica

Microbiota and diabetes: an increasingly relevant association

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